Cargando…

High Frequency of Class 1 Integrons in Escherichia coli Isolated From Patients With Urinary Tract Infections in Yasuj, Iran

BACKGROUND: Most urinary tract infections (UTI) are caused by Escherichia coli. Integrons have an important role in distributing antibiotic resistance genes among bacteria. OBJECTIVES: The aim of this study was to investigate the presence of class 1, 2 and 3 integrons and their association with anti...

Descripción completa

Detalles Bibliográficos
Autores principales: Khoramrooz, Seyed Sajjad, Sharifi, Asghar, Yazdanpanah, Mahboubeh, Malek Hosseini, Seyed Ali Asghar, Emaneini, Mohammad, Gharibpour, Farzaneh, Parhizgari, Najmeh, Mirzaii, Mehdi, Zoladl, Mohammad, Khosravani, Seyed Abdolmajid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752967/
https://www.ncbi.nlm.nih.gov/pubmed/26889395
http://dx.doi.org/10.5812/ircmj.26399
_version_ 1782415808080642048
author Khoramrooz, Seyed Sajjad
Sharifi, Asghar
Yazdanpanah, Mahboubeh
Malek Hosseini, Seyed Ali Asghar
Emaneini, Mohammad
Gharibpour, Farzaneh
Parhizgari, Najmeh
Mirzaii, Mehdi
Zoladl, Mohammad
Khosravani, Seyed Abdolmajid
author_facet Khoramrooz, Seyed Sajjad
Sharifi, Asghar
Yazdanpanah, Mahboubeh
Malek Hosseini, Seyed Ali Asghar
Emaneini, Mohammad
Gharibpour, Farzaneh
Parhizgari, Najmeh
Mirzaii, Mehdi
Zoladl, Mohammad
Khosravani, Seyed Abdolmajid
author_sort Khoramrooz, Seyed Sajjad
collection PubMed
description BACKGROUND: Most urinary tract infections (UTI) are caused by Escherichia coli. Integrons have an important role in distributing antibiotic resistance genes among bacteria. OBJECTIVES: The aim of this study was to investigate the presence of class 1, 2 and 3 integrons and their association with antibiotic resistance in E. coli isolated from patient with UTI in Yasuj, Iran. PATIENTS AND METHODS: In this cross-sectional study a total of 200 E. coli were collected from 1820 patients diagnosed with UTI that had been referred to two clinical laboratories between February 2013 and November 2014 in Yasuj city, southwest of Iran. Susceptibility of isolates to 11 different antibiotics was determined by the disk agar diffusion method. multiplex-polymerase chain reaction (PCR) was used for detection of class 1, 2 and 3 integrons. The data were analyzed using the SPSS software (version 16) and the chi-square test. A P value of < 0.05 was considered statistically significant. RESULTS: The highest rate of resistance was observed toward cephalothin (99%) and amoxicillin (76%) while only two (1%) isolates showed resistance to imipenem. Overall, 79% of isolates were multi drug resistant (MDR). Class 1 and 2 integrons were detected in 104 (52%) and 5 (2.5%) isolates respectively, while none of the isolates were positive for class 3 integrons. A significant association was observed between the presence of integrons and resistance to co-trimoxazole, nalidixic acid, ciprofloxacin, amoxicillin, ceftazidime and tetracycline (P < 0.05). CONCLUSIONS: High MDR isolates of E. coli were observed in this study. The significant association between class 1 integrons and resistance to ciprofloxacin, nalidixic acid, co-trimoxazole, amoxicillin, ceftazidime and tetracycline showed that class 1 integrons have an important role in resistance to these antibiotics in this region.
format Online
Article
Text
id pubmed-4752967
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Kowsar
record_format MEDLINE/PubMed
spelling pubmed-47529672016-02-17 High Frequency of Class 1 Integrons in Escherichia coli Isolated From Patients With Urinary Tract Infections in Yasuj, Iran Khoramrooz, Seyed Sajjad Sharifi, Asghar Yazdanpanah, Mahboubeh Malek Hosseini, Seyed Ali Asghar Emaneini, Mohammad Gharibpour, Farzaneh Parhizgari, Najmeh Mirzaii, Mehdi Zoladl, Mohammad Khosravani, Seyed Abdolmajid Iran Red Crescent Med J Research Article BACKGROUND: Most urinary tract infections (UTI) are caused by Escherichia coli. Integrons have an important role in distributing antibiotic resistance genes among bacteria. OBJECTIVES: The aim of this study was to investigate the presence of class 1, 2 and 3 integrons and their association with antibiotic resistance in E. coli isolated from patient with UTI in Yasuj, Iran. PATIENTS AND METHODS: In this cross-sectional study a total of 200 E. coli were collected from 1820 patients diagnosed with UTI that had been referred to two clinical laboratories between February 2013 and November 2014 in Yasuj city, southwest of Iran. Susceptibility of isolates to 11 different antibiotics was determined by the disk agar diffusion method. multiplex-polymerase chain reaction (PCR) was used for detection of class 1, 2 and 3 integrons. The data were analyzed using the SPSS software (version 16) and the chi-square test. A P value of < 0.05 was considered statistically significant. RESULTS: The highest rate of resistance was observed toward cephalothin (99%) and amoxicillin (76%) while only two (1%) isolates showed resistance to imipenem. Overall, 79% of isolates were multi drug resistant (MDR). Class 1 and 2 integrons were detected in 104 (52%) and 5 (2.5%) isolates respectively, while none of the isolates were positive for class 3 integrons. A significant association was observed between the presence of integrons and resistance to co-trimoxazole, nalidixic acid, ciprofloxacin, amoxicillin, ceftazidime and tetracycline (P < 0.05). CONCLUSIONS: High MDR isolates of E. coli were observed in this study. The significant association between class 1 integrons and resistance to ciprofloxacin, nalidixic acid, co-trimoxazole, amoxicillin, ceftazidime and tetracycline showed that class 1 integrons have an important role in resistance to these antibiotics in this region. Kowsar 2016-01-13 /pmc/articles/PMC4752967/ /pubmed/26889395 http://dx.doi.org/10.5812/ircmj.26399 Text en Copyright © 2016, Iranian Red Crescent Medical Journal. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Research Article
Khoramrooz, Seyed Sajjad
Sharifi, Asghar
Yazdanpanah, Mahboubeh
Malek Hosseini, Seyed Ali Asghar
Emaneini, Mohammad
Gharibpour, Farzaneh
Parhizgari, Najmeh
Mirzaii, Mehdi
Zoladl, Mohammad
Khosravani, Seyed Abdolmajid
High Frequency of Class 1 Integrons in Escherichia coli Isolated From Patients With Urinary Tract Infections in Yasuj, Iran
title High Frequency of Class 1 Integrons in Escherichia coli Isolated From Patients With Urinary Tract Infections in Yasuj, Iran
title_full High Frequency of Class 1 Integrons in Escherichia coli Isolated From Patients With Urinary Tract Infections in Yasuj, Iran
title_fullStr High Frequency of Class 1 Integrons in Escherichia coli Isolated From Patients With Urinary Tract Infections in Yasuj, Iran
title_full_unstemmed High Frequency of Class 1 Integrons in Escherichia coli Isolated From Patients With Urinary Tract Infections in Yasuj, Iran
title_short High Frequency of Class 1 Integrons in Escherichia coli Isolated From Patients With Urinary Tract Infections in Yasuj, Iran
title_sort high frequency of class 1 integrons in escherichia coli isolated from patients with urinary tract infections in yasuj, iran
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752967/
https://www.ncbi.nlm.nih.gov/pubmed/26889395
http://dx.doi.org/10.5812/ircmj.26399
work_keys_str_mv AT khoramroozseyedsajjad highfrequencyofclass1integronsinescherichiacoliisolatedfrompatientswithurinarytractinfectionsinyasujiran
AT sharifiasghar highfrequencyofclass1integronsinescherichiacoliisolatedfrompatientswithurinarytractinfectionsinyasujiran
AT yazdanpanahmahboubeh highfrequencyofclass1integronsinescherichiacoliisolatedfrompatientswithurinarytractinfectionsinyasujiran
AT malekhosseiniseyedaliasghar highfrequencyofclass1integronsinescherichiacoliisolatedfrompatientswithurinarytractinfectionsinyasujiran
AT emaneinimohammad highfrequencyofclass1integronsinescherichiacoliisolatedfrompatientswithurinarytractinfectionsinyasujiran
AT gharibpourfarzaneh highfrequencyofclass1integronsinescherichiacoliisolatedfrompatientswithurinarytractinfectionsinyasujiran
AT parhizgarinajmeh highfrequencyofclass1integronsinescherichiacoliisolatedfrompatientswithurinarytractinfectionsinyasujiran
AT mirzaiimehdi highfrequencyofclass1integronsinescherichiacoliisolatedfrompatientswithurinarytractinfectionsinyasujiran
AT zoladlmohammad highfrequencyofclass1integronsinescherichiacoliisolatedfrompatientswithurinarytractinfectionsinyasujiran
AT khosravaniseyedabdolmajid highfrequencyofclass1integronsinescherichiacoliisolatedfrompatientswithurinarytractinfectionsinyasujiran