Cargando…

Downregulation of Mitofusin 2 in Placenta Is Related to Preeclampsia

Background. Mitofusin 2 (Mfn2) is a novel mitochondrial protein that is implicated in cellular proliferation and metabolism; however, the role of Mfn2 in preeclampsia (PE) remains unknown. This study aimed to explore the relationship between Mfn2 and PE. Method. Preeclamptic and normal pregnancies w...

Descripción completa

Detalles Bibliográficos
Autores principales: Yu, Jun, Guo, Xijiao, Chen, Ruibao, Feng, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752976/
https://www.ncbi.nlm.nih.gov/pubmed/26942197
http://dx.doi.org/10.1155/2016/6323086
_version_ 1782415810100199424
author Yu, Jun
Guo, Xijiao
Chen, Ruibao
Feng, Ling
author_facet Yu, Jun
Guo, Xijiao
Chen, Ruibao
Feng, Ling
author_sort Yu, Jun
collection PubMed
description Background. Mitofusin 2 (Mfn2) is a novel mitochondrial protein that is implicated in cellular proliferation and metabolism; however, the role of Mfn2 in preeclampsia (PE) remains unknown. This study aimed to explore the relationship between Mfn2 and PE. Method. Preeclamptic and normal pregnancies were enrolled in a comparative study. The expression of Mfn2 in placenta was detected by qRT-PCR. And the mitochondrial function was detected by ATP assay. Then TEV-1 cells were cultured in hypoxic conditions. mRNA and protein expressions of Mfn2 were detected by qRT-PCR and western blot separately. Cells' viability was detected by MTT. And the mitochondrial function was detected by ATP and mitochondrial membrane potential (MMP) assay. We further knocked down the Mfn2 gene in TEV-1 cells and evaluated the cells' viability. Results. Mfn2 and ATP expressions were significantly decreased in preeclamptic placentae compared to normal placentae. Mfn2 expression level and the viability of TEV-1 cells were reduced during hypoxic conditions. TEV-1 cells' viability, ATP, and MMP levels were also significantly decreased after knockdown of the Mfn2 gene. Conclusions. These results suggest that defects in Mfn2 could cause mitochondrial dysfunction and decrease trophoblastic cells' viability. Therefore, Mfn2 may be functionally involved in the pathogenesis of PE.
format Online
Article
Text
id pubmed-4752976
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-47529762016-03-03 Downregulation of Mitofusin 2 in Placenta Is Related to Preeclampsia Yu, Jun Guo, Xijiao Chen, Ruibao Feng, Ling Biomed Res Int Research Article Background. Mitofusin 2 (Mfn2) is a novel mitochondrial protein that is implicated in cellular proliferation and metabolism; however, the role of Mfn2 in preeclampsia (PE) remains unknown. This study aimed to explore the relationship between Mfn2 and PE. Method. Preeclamptic and normal pregnancies were enrolled in a comparative study. The expression of Mfn2 in placenta was detected by qRT-PCR. And the mitochondrial function was detected by ATP assay. Then TEV-1 cells were cultured in hypoxic conditions. mRNA and protein expressions of Mfn2 were detected by qRT-PCR and western blot separately. Cells' viability was detected by MTT. And the mitochondrial function was detected by ATP and mitochondrial membrane potential (MMP) assay. We further knocked down the Mfn2 gene in TEV-1 cells and evaluated the cells' viability. Results. Mfn2 and ATP expressions were significantly decreased in preeclamptic placentae compared to normal placentae. Mfn2 expression level and the viability of TEV-1 cells were reduced during hypoxic conditions. TEV-1 cells' viability, ATP, and MMP levels were also significantly decreased after knockdown of the Mfn2 gene. Conclusions. These results suggest that defects in Mfn2 could cause mitochondrial dysfunction and decrease trophoblastic cells' viability. Therefore, Mfn2 may be functionally involved in the pathogenesis of PE. Hindawi Publishing Corporation 2016 2016-01-31 /pmc/articles/PMC4752976/ /pubmed/26942197 http://dx.doi.org/10.1155/2016/6323086 Text en Copyright © 2016 Jun Yu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yu, Jun
Guo, Xijiao
Chen, Ruibao
Feng, Ling
Downregulation of Mitofusin 2 in Placenta Is Related to Preeclampsia
title Downregulation of Mitofusin 2 in Placenta Is Related to Preeclampsia
title_full Downregulation of Mitofusin 2 in Placenta Is Related to Preeclampsia
title_fullStr Downregulation of Mitofusin 2 in Placenta Is Related to Preeclampsia
title_full_unstemmed Downregulation of Mitofusin 2 in Placenta Is Related to Preeclampsia
title_short Downregulation of Mitofusin 2 in Placenta Is Related to Preeclampsia
title_sort downregulation of mitofusin 2 in placenta is related to preeclampsia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4752976/
https://www.ncbi.nlm.nih.gov/pubmed/26942197
http://dx.doi.org/10.1155/2016/6323086
work_keys_str_mv AT yujun downregulationofmitofusin2inplacentaisrelatedtopreeclampsia
AT guoxijiao downregulationofmitofusin2inplacentaisrelatedtopreeclampsia
AT chenruibao downregulationofmitofusin2inplacentaisrelatedtopreeclampsia
AT fengling downregulationofmitofusin2inplacentaisrelatedtopreeclampsia