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Chromatin Ring Formation at Plant Centromeres

We observed the formation of chromatin ring structures at centromeres of somatic rye and Arabidopsis chromosomes. To test whether this behavior is present also in other plant species and tissues we analyzed Arabidopsis, rye, wheat, Aegilops and barley centromeres during cell divisions and in interph...

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Detalles Bibliográficos
Autores principales: Schubert, Veit, Ruban, Alevtina, Houben, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753331/
https://www.ncbi.nlm.nih.gov/pubmed/26913037
http://dx.doi.org/10.3389/fpls.2016.00028
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author Schubert, Veit
Ruban, Alevtina
Houben, Andreas
author_facet Schubert, Veit
Ruban, Alevtina
Houben, Andreas
author_sort Schubert, Veit
collection PubMed
description We observed the formation of chromatin ring structures at centromeres of somatic rye and Arabidopsis chromosomes. To test whether this behavior is present also in other plant species and tissues we analyzed Arabidopsis, rye, wheat, Aegilops and barley centromeres during cell divisions and in interphase nuclei by immunostaining and FISH. Furthermore, structured illumination microscopy (super-resolution) was applied to investigate the ultrastructure of centromere chromatin beyond the classical refraction limit of light. It became obvious, that a ring formation at centromeres may appear during mitosis, meiosis and in interphase nuclei in all species analyzed. However, varying centromere structures, as ring formations or globular organized chromatin fibers, were identified in different tissues of one and the same species. In addition, we found that a chromatin ring formation may also be caused by subtelomeric repeats in barley. Thus, we conclude that the formation of chromatin rings may appear in different plant species and tissues, but that it is not specific for centromere function. Based on our findings we established a model describing the ultrastructure of plant centromeres and discuss it in comparison to previous models proposed for animals and plants.
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spelling pubmed-47533312016-02-24 Chromatin Ring Formation at Plant Centromeres Schubert, Veit Ruban, Alevtina Houben, Andreas Front Plant Sci Plant Science We observed the formation of chromatin ring structures at centromeres of somatic rye and Arabidopsis chromosomes. To test whether this behavior is present also in other plant species and tissues we analyzed Arabidopsis, rye, wheat, Aegilops and barley centromeres during cell divisions and in interphase nuclei by immunostaining and FISH. Furthermore, structured illumination microscopy (super-resolution) was applied to investigate the ultrastructure of centromere chromatin beyond the classical refraction limit of light. It became obvious, that a ring formation at centromeres may appear during mitosis, meiosis and in interphase nuclei in all species analyzed. However, varying centromere structures, as ring formations or globular organized chromatin fibers, were identified in different tissues of one and the same species. In addition, we found that a chromatin ring formation may also be caused by subtelomeric repeats in barley. Thus, we conclude that the formation of chromatin rings may appear in different plant species and tissues, but that it is not specific for centromere function. Based on our findings we established a model describing the ultrastructure of plant centromeres and discuss it in comparison to previous models proposed for animals and plants. Frontiers Media S.A. 2016-02-15 /pmc/articles/PMC4753331/ /pubmed/26913037 http://dx.doi.org/10.3389/fpls.2016.00028 Text en Copyright © 2016 Schubert, Ruban and Houben. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Schubert, Veit
Ruban, Alevtina
Houben, Andreas
Chromatin Ring Formation at Plant Centromeres
title Chromatin Ring Formation at Plant Centromeres
title_full Chromatin Ring Formation at Plant Centromeres
title_fullStr Chromatin Ring Formation at Plant Centromeres
title_full_unstemmed Chromatin Ring Formation at Plant Centromeres
title_short Chromatin Ring Formation at Plant Centromeres
title_sort chromatin ring formation at plant centromeres
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753331/
https://www.ncbi.nlm.nih.gov/pubmed/26913037
http://dx.doi.org/10.3389/fpls.2016.00028
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