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Feminization and masculinization of western mosquitofish (Gambusia affinis) observed in rivers impacted by municipal wastewaters
Municipal wastewaters have been known to contain various estrogens and androgens. Little is known about the joint action of these chemicals from wastewaters on fishes in the aquatic environment. The objectives of this study were to investigate the estrogenic and/or androgenic effects in wild mosquit...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753483/ https://www.ncbi.nlm.nih.gov/pubmed/26876344 http://dx.doi.org/10.1038/srep20884 |
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author | Huang, Guo-Yong Liu, You-Sheng Chen, Xiao-Wen Liang, Yan-Qiu Liu, Shuang-Shuang Yang, Yuan-Yuan Hu, Li-Xin Shi, Wen-Jun Tian, Fei Zhao, Jian-Liang Chen, Jun Ying, Guang-Guo |
author_facet | Huang, Guo-Yong Liu, You-Sheng Chen, Xiao-Wen Liang, Yan-Qiu Liu, Shuang-Shuang Yang, Yuan-Yuan Hu, Li-Xin Shi, Wen-Jun Tian, Fei Zhao, Jian-Liang Chen, Jun Ying, Guang-Guo |
author_sort | Huang, Guo-Yong |
collection | PubMed |
description | Municipal wastewaters have been known to contain various estrogens and androgens. Little is known about the joint action of these chemicals from wastewaters on fishes in the aquatic environment. The objectives of this study were to investigate the estrogenic and/or androgenic effects in wild mosquitofish (Gambusia affinis) of two effluent-impacted rivers in South China by determining morphological changes and hepatic mRNA expression levels of relevant genes such as vitellogenin (Vtg), estrogen receptor (ERα) and androgen receptors (ARα and ARβ), and to assess the linkages of those morphological changes and hepatic mRNA expression levels to the chemical concentrations measured by in vitro bioassays and chemical analysis. The results showed a significant induction of Vtg and ERα mRNA in the livers of the males and a gonopodium-like anal fin in the females collected at the majority of sites. Redundancy analysis and Pearson correlation analysis showed that the chemical concentrations obtained by in vitro bioassays and chemical analysis had significant correlations with some of the endpoints for the estrogenic and/or androgenic effects in mosquitofish. The findings from this study indicate that the estrogens and androgens present in the two rivers could cause the observed estrogenic and androgenic effects in mosquitofish. |
format | Online Article Text |
id | pubmed-4753483 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47534832016-02-23 Feminization and masculinization of western mosquitofish (Gambusia affinis) observed in rivers impacted by municipal wastewaters Huang, Guo-Yong Liu, You-Sheng Chen, Xiao-Wen Liang, Yan-Qiu Liu, Shuang-Shuang Yang, Yuan-Yuan Hu, Li-Xin Shi, Wen-Jun Tian, Fei Zhao, Jian-Liang Chen, Jun Ying, Guang-Guo Sci Rep Article Municipal wastewaters have been known to contain various estrogens and androgens. Little is known about the joint action of these chemicals from wastewaters on fishes in the aquatic environment. The objectives of this study were to investigate the estrogenic and/or androgenic effects in wild mosquitofish (Gambusia affinis) of two effluent-impacted rivers in South China by determining morphological changes and hepatic mRNA expression levels of relevant genes such as vitellogenin (Vtg), estrogen receptor (ERα) and androgen receptors (ARα and ARβ), and to assess the linkages of those morphological changes and hepatic mRNA expression levels to the chemical concentrations measured by in vitro bioassays and chemical analysis. The results showed a significant induction of Vtg and ERα mRNA in the livers of the males and a gonopodium-like anal fin in the females collected at the majority of sites. Redundancy analysis and Pearson correlation analysis showed that the chemical concentrations obtained by in vitro bioassays and chemical analysis had significant correlations with some of the endpoints for the estrogenic and/or androgenic effects in mosquitofish. The findings from this study indicate that the estrogens and androgens present in the two rivers could cause the observed estrogenic and androgenic effects in mosquitofish. Nature Publishing Group 2016-02-15 /pmc/articles/PMC4753483/ /pubmed/26876344 http://dx.doi.org/10.1038/srep20884 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Huang, Guo-Yong Liu, You-Sheng Chen, Xiao-Wen Liang, Yan-Qiu Liu, Shuang-Shuang Yang, Yuan-Yuan Hu, Li-Xin Shi, Wen-Jun Tian, Fei Zhao, Jian-Liang Chen, Jun Ying, Guang-Guo Feminization and masculinization of western mosquitofish (Gambusia affinis) observed in rivers impacted by municipal wastewaters |
title | Feminization and masculinization of western mosquitofish (Gambusia affinis) observed in rivers impacted by municipal wastewaters |
title_full | Feminization and masculinization of western mosquitofish (Gambusia affinis) observed in rivers impacted by municipal wastewaters |
title_fullStr | Feminization and masculinization of western mosquitofish (Gambusia affinis) observed in rivers impacted by municipal wastewaters |
title_full_unstemmed | Feminization and masculinization of western mosquitofish (Gambusia affinis) observed in rivers impacted by municipal wastewaters |
title_short | Feminization and masculinization of western mosquitofish (Gambusia affinis) observed in rivers impacted by municipal wastewaters |
title_sort | feminization and masculinization of western mosquitofish (gambusia affinis) observed in rivers impacted by municipal wastewaters |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753483/ https://www.ncbi.nlm.nih.gov/pubmed/26876344 http://dx.doi.org/10.1038/srep20884 |
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