Cargando…

A genome-wide association study of a global rice panel reveals resistance in Oryza sativa to root-knot nematodes

The root-knot nematode Meloidogyne graminicola is one of the most serious nematode pests worldwide and represents a major constraint on rice production. While variation in the susceptibility of Asian rice (Oryza sativa) exists, so far no strong and reliable resistance has been reported. Quantitative...

Descripción completa

Detalles Bibliográficos
Autores principales: Dimkpa, Stanley O. N., Lahari, Zobaida, Shrestha, Roshi, Douglas, Alex, Gheysen, Godelieve, Price, Adam H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753847/
https://www.ncbi.nlm.nih.gov/pubmed/26552884
http://dx.doi.org/10.1093/jxb/erv470
_version_ 1782415923298172928
author Dimkpa, Stanley O. N.
Lahari, Zobaida
Shrestha, Roshi
Douglas, Alex
Gheysen, Godelieve
Price, Adam H.
author_facet Dimkpa, Stanley O. N.
Lahari, Zobaida
Shrestha, Roshi
Douglas, Alex
Gheysen, Godelieve
Price, Adam H.
author_sort Dimkpa, Stanley O. N.
collection PubMed
description The root-knot nematode Meloidogyne graminicola is one of the most serious nematode pests worldwide and represents a major constraint on rice production. While variation in the susceptibility of Asian rice (Oryza sativa) exists, so far no strong and reliable resistance has been reported. Quantitative trait loci for partial resistance have been reported but no underlying genes have been tagged or cloned. Here, 332 accessions of the Rice Diversity Panel 1 were assessed for gall formation, revealing large variation across all subpopulations of rice and higher susceptibility in temperate japonica accessions. Accessions Khao Pahk Maw and LD 24 appeared to be resistant, which was confirmed in large pot experiments where no galls were observed. Detailed observations on these two accessions revealed no nematodes inside the roots 2 days after inoculation and very few females after 17 days (5 in Khao Pahk Maw and <1 in LD 24, in comparison with >100 in the susceptible controls). These two cultivars appear ideal donors for breeding root-knot nematode resistance. A genome-wide association study revealed 11 quantitative trait loci, two of which are close to epistatic loci detected in the Bala x Azucena population. The discussion highlights a small number of candidate genes worth exploring further, in particular many genes with lectin domains and genes on chromosome 11 with homology to the Hordeum Mla locus.
format Online
Article
Text
id pubmed-4753847
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-47538472016-02-16 A genome-wide association study of a global rice panel reveals resistance in Oryza sativa to root-knot nematodes Dimkpa, Stanley O. N. Lahari, Zobaida Shrestha, Roshi Douglas, Alex Gheysen, Godelieve Price, Adam H. J Exp Bot Research Paper The root-knot nematode Meloidogyne graminicola is one of the most serious nematode pests worldwide and represents a major constraint on rice production. While variation in the susceptibility of Asian rice (Oryza sativa) exists, so far no strong and reliable resistance has been reported. Quantitative trait loci for partial resistance have been reported but no underlying genes have been tagged or cloned. Here, 332 accessions of the Rice Diversity Panel 1 were assessed for gall formation, revealing large variation across all subpopulations of rice and higher susceptibility in temperate japonica accessions. Accessions Khao Pahk Maw and LD 24 appeared to be resistant, which was confirmed in large pot experiments where no galls were observed. Detailed observations on these two accessions revealed no nematodes inside the roots 2 days after inoculation and very few females after 17 days (5 in Khao Pahk Maw and <1 in LD 24, in comparison with >100 in the susceptible controls). These two cultivars appear ideal donors for breeding root-knot nematode resistance. A genome-wide association study revealed 11 quantitative trait loci, two of which are close to epistatic loci detected in the Bala x Azucena population. The discussion highlights a small number of candidate genes worth exploring further, in particular many genes with lectin domains and genes on chromosome 11 with homology to the Hordeum Mla locus. Oxford University Press 2016-02 2015-11-09 /pmc/articles/PMC4753847/ /pubmed/26552884 http://dx.doi.org/10.1093/jxb/erv470 Text en © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Dimkpa, Stanley O. N.
Lahari, Zobaida
Shrestha, Roshi
Douglas, Alex
Gheysen, Godelieve
Price, Adam H.
A genome-wide association study of a global rice panel reveals resistance in Oryza sativa to root-knot nematodes
title A genome-wide association study of a global rice panel reveals resistance in Oryza sativa to root-knot nematodes
title_full A genome-wide association study of a global rice panel reveals resistance in Oryza sativa to root-knot nematodes
title_fullStr A genome-wide association study of a global rice panel reveals resistance in Oryza sativa to root-knot nematodes
title_full_unstemmed A genome-wide association study of a global rice panel reveals resistance in Oryza sativa to root-knot nematodes
title_short A genome-wide association study of a global rice panel reveals resistance in Oryza sativa to root-knot nematodes
title_sort genome-wide association study of a global rice panel reveals resistance in oryza sativa to root-knot nematodes
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753847/
https://www.ncbi.nlm.nih.gov/pubmed/26552884
http://dx.doi.org/10.1093/jxb/erv470
work_keys_str_mv AT dimkpastanleyon agenomewideassociationstudyofaglobalricepanelrevealsresistanceinoryzasativatorootknotnematodes
AT laharizobaida agenomewideassociationstudyofaglobalricepanelrevealsresistanceinoryzasativatorootknotnematodes
AT shrestharoshi agenomewideassociationstudyofaglobalricepanelrevealsresistanceinoryzasativatorootknotnematodes
AT douglasalex agenomewideassociationstudyofaglobalricepanelrevealsresistanceinoryzasativatorootknotnematodes
AT gheysengodelieve agenomewideassociationstudyofaglobalricepanelrevealsresistanceinoryzasativatorootknotnematodes
AT priceadamh agenomewideassociationstudyofaglobalricepanelrevealsresistanceinoryzasativatorootknotnematodes
AT dimkpastanleyon genomewideassociationstudyofaglobalricepanelrevealsresistanceinoryzasativatorootknotnematodes
AT laharizobaida genomewideassociationstudyofaglobalricepanelrevealsresistanceinoryzasativatorootknotnematodes
AT shrestharoshi genomewideassociationstudyofaglobalricepanelrevealsresistanceinoryzasativatorootknotnematodes
AT douglasalex genomewideassociationstudyofaglobalricepanelrevealsresistanceinoryzasativatorootknotnematodes
AT gheysengodelieve genomewideassociationstudyofaglobalricepanelrevealsresistanceinoryzasativatorootknotnematodes
AT priceadamh genomewideassociationstudyofaglobalricepanelrevealsresistanceinoryzasativatorootknotnematodes