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Upregulation of miRNA-130a Represents Good Prognosis in Patients With HBV-Related Acute-on-Chronic Liver Failure: A Prospective Study

Prompt and accurate prediction of the outcome is the key to make correct medical decision and to reduce the mortality in patients with HBV-related acute-on-chronic liver failure (ACLF). Increasing evidence have certified that small, noncoding microRNAs (miRNAs) play critically regulatory roles in th...

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Autores principales: Zheng, Qing-Fen, Zhang, Jing-Yun, Wu, Ju-Shan, Zhang, Ying, Liu, Mei, Bai, Li, Zhang, Jin-Yan, Zhao, Jing, Chen, Yu, Duan, Zhong-Ping, Zheng, Su-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer Health 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753881/
https://www.ncbi.nlm.nih.gov/pubmed/26871786
http://dx.doi.org/10.1097/MD.0000000000002639
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author Zheng, Qing-Fen
Zhang, Jing-Yun
Wu, Ju-Shan
Zhang, Ying
Liu, Mei
Bai, Li
Zhang, Jin-Yan
Zhao, Jing
Chen, Yu
Duan, Zhong-Ping
Zheng, Su-Jun
author_facet Zheng, Qing-Fen
Zhang, Jing-Yun
Wu, Ju-Shan
Zhang, Ying
Liu, Mei
Bai, Li
Zhang, Jin-Yan
Zhao, Jing
Chen, Yu
Duan, Zhong-Ping
Zheng, Su-Jun
author_sort Zheng, Qing-Fen
collection PubMed
description Prompt and accurate prediction of the outcome is the key to make correct medical decision and to reduce the mortality in patients with HBV-related acute-on-chronic liver failure (ACLF). Increasing evidence have certified that small, noncoding microRNAs (miRNAs) play critically regulatory roles in the pathogenesis of liver diseases. However, it remains unclear whether and how miRNAs involve in the prognosis of ACLF. Microarray analysis was performed to characterize the miRNA expression profiles in liver tissues from 1 HBV-related ACLF patient and 1 matched healthy control. Nine miRNAs with at least 5 folds difference between these 2 persons were picked out. The present prospective study involving 39 HBV-related ACLF patients including 20 recovered and 19 nonrecovered patients, which include death (n = 9) and liver transplantation (n = 10). The serum expression of these miRNAs detected by quantitative real-time Polymerase Chain Reaction (qRT-RCR) was then compared between the 2 groups. Moreover, the correlation between the serum miRNAs and the prognostic indexes for ACLF was analyzed. The result of microarray analysis showed 9 miRNAs had different expression in liver tissues of ACLF patient compared with healthy control (upregulated: miRNA-130a, −21, −143, and −200a; downregulated: miRNA-486–5p, −192, −148a, −122, and −194). Unlike the expression profiles in liver tissue, 8 serum miRNAs except miRNA-194 were markedly upregulated in ACLF patients (P < 0.05). Remarkably, the serum expression of miRNA-130a and miRNA-486–5p was higher in recovered than nonrecovered ACLF patients (P < 0.05). Especially, the serum miRNA-130a was negatively correlated with international normalized ratio, prothrombin time, Model for End-Stage Liver Disease score, and positively correlated with prothrombin time activity. The AUC for recovered versus nonrecovered patients of miRNA-130a was 0.741 (P = 0.02). miRNA-130a might be a useful prognosis biomarker in patients with HBV-related ACLF.
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spelling pubmed-47538812016-02-26 Upregulation of miRNA-130a Represents Good Prognosis in Patients With HBV-Related Acute-on-Chronic Liver Failure: A Prospective Study Zheng, Qing-Fen Zhang, Jing-Yun Wu, Ju-Shan Zhang, Ying Liu, Mei Bai, Li Zhang, Jin-Yan Zhao, Jing Chen, Yu Duan, Zhong-Ping Zheng, Su-Jun Medicine (Baltimore) 4500 Prompt and accurate prediction of the outcome is the key to make correct medical decision and to reduce the mortality in patients with HBV-related acute-on-chronic liver failure (ACLF). Increasing evidence have certified that small, noncoding microRNAs (miRNAs) play critically regulatory roles in the pathogenesis of liver diseases. However, it remains unclear whether and how miRNAs involve in the prognosis of ACLF. Microarray analysis was performed to characterize the miRNA expression profiles in liver tissues from 1 HBV-related ACLF patient and 1 matched healthy control. Nine miRNAs with at least 5 folds difference between these 2 persons were picked out. The present prospective study involving 39 HBV-related ACLF patients including 20 recovered and 19 nonrecovered patients, which include death (n = 9) and liver transplantation (n = 10). The serum expression of these miRNAs detected by quantitative real-time Polymerase Chain Reaction (qRT-RCR) was then compared between the 2 groups. Moreover, the correlation between the serum miRNAs and the prognostic indexes for ACLF was analyzed. The result of microarray analysis showed 9 miRNAs had different expression in liver tissues of ACLF patient compared with healthy control (upregulated: miRNA-130a, −21, −143, and −200a; downregulated: miRNA-486–5p, −192, −148a, −122, and −194). Unlike the expression profiles in liver tissue, 8 serum miRNAs except miRNA-194 were markedly upregulated in ACLF patients (P < 0.05). Remarkably, the serum expression of miRNA-130a and miRNA-486–5p was higher in recovered than nonrecovered ACLF patients (P < 0.05). Especially, the serum miRNA-130a was negatively correlated with international normalized ratio, prothrombin time, Model for End-Stage Liver Disease score, and positively correlated with prothrombin time activity. The AUC for recovered versus nonrecovered patients of miRNA-130a was 0.741 (P = 0.02). miRNA-130a might be a useful prognosis biomarker in patients with HBV-related ACLF. Wolters Kluwer Health 2016-02-12 /pmc/articles/PMC4753881/ /pubmed/26871786 http://dx.doi.org/10.1097/MD.0000000000002639 Text en Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved. http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0
spellingShingle 4500
Zheng, Qing-Fen
Zhang, Jing-Yun
Wu, Ju-Shan
Zhang, Ying
Liu, Mei
Bai, Li
Zhang, Jin-Yan
Zhao, Jing
Chen, Yu
Duan, Zhong-Ping
Zheng, Su-Jun
Upregulation of miRNA-130a Represents Good Prognosis in Patients With HBV-Related Acute-on-Chronic Liver Failure: A Prospective Study
title Upregulation of miRNA-130a Represents Good Prognosis in Patients With HBV-Related Acute-on-Chronic Liver Failure: A Prospective Study
title_full Upregulation of miRNA-130a Represents Good Prognosis in Patients With HBV-Related Acute-on-Chronic Liver Failure: A Prospective Study
title_fullStr Upregulation of miRNA-130a Represents Good Prognosis in Patients With HBV-Related Acute-on-Chronic Liver Failure: A Prospective Study
title_full_unstemmed Upregulation of miRNA-130a Represents Good Prognosis in Patients With HBV-Related Acute-on-Chronic Liver Failure: A Prospective Study
title_short Upregulation of miRNA-130a Represents Good Prognosis in Patients With HBV-Related Acute-on-Chronic Liver Failure: A Prospective Study
title_sort upregulation of mirna-130a represents good prognosis in patients with hbv-related acute-on-chronic liver failure: a prospective study
topic 4500
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4753881/
https://www.ncbi.nlm.nih.gov/pubmed/26871786
http://dx.doi.org/10.1097/MD.0000000000002639
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