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Elevated urinary urea by high-protein diet could be one of the inducements of bladder disorders
BACKGROUND: Previous work found that urea accumulation in urothelial cells caused by urea transporter B knockout led to DNA damage and apoptosis that contributed to the carcinogenesis. The purpose of this study is to explore the potential connection between high urinary urea concentration and the bl...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4755000/ https://www.ncbi.nlm.nih.gov/pubmed/26879937 http://dx.doi.org/10.1186/s12967-016-0809-9 |
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author | Liu, Ming Li, Min Liu, Jiangfeng Wang, Hongkai Zhong, Dandan Zhou, Hong Yang, Baoxue |
author_facet | Liu, Ming Li, Min Liu, Jiangfeng Wang, Hongkai Zhong, Dandan Zhou, Hong Yang, Baoxue |
author_sort | Liu, Ming |
collection | PubMed |
description | BACKGROUND: Previous work found that urea accumulation in urothelial cells caused by urea transporter B knockout led to DNA damage and apoptosis that contributed to the carcinogenesis. The purpose of this study is to explore the potential connection between high urinary urea concentration and the bladder disorders. METHODS: A high protein diet rat model was conducted by feeding with 40 % protein diet. In-silico modeling and algorithm, based on the results of microarray and proteomics from the bladder urothelium, were used for the reconstruction of accurate cellular networks and the identification of novel master regulators in the high-protein diet rat model. Pathway and biological process enrichment analysis were used to characterize predicted targets of candidate mRNAs/proteins. The expression pattern of the most significant master regulators was evaluated by qPCR and immunohistochemistry. RESULTS: Based on the analysis of different expressed mRNAs/proteins, 15 significant ones (CRP, MCPT2, MCPT9, EPXH2, SERPING1, SRGN, CDKN1C, CDK6, CCNB1, PCNA, BAX, MAGEB16, SERPINE1, HSPA2, FOS) were highly identified and verified by qPCR and immunohistochemistry. They were involved in immune and inflammatory response, cell cycle arrest, apoptosis and pathways in cancer. These abnormally activated processes caused the bladder interstitial congestion and inflammatory infiltrates under the thinner urothelium, cell desquamation, cytoplasm vacuolization, nucleus swelling and malformation in the high-protein diet group. CONCLUSIONS: We provided evidences that high urinary urea concentration caused by high-protein diet might be a potential carcinogenic factor in bladder. |
format | Online Article Text |
id | pubmed-4755000 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-47550002016-02-17 Elevated urinary urea by high-protein diet could be one of the inducements of bladder disorders Liu, Ming Li, Min Liu, Jiangfeng Wang, Hongkai Zhong, Dandan Zhou, Hong Yang, Baoxue J Transl Med Research BACKGROUND: Previous work found that urea accumulation in urothelial cells caused by urea transporter B knockout led to DNA damage and apoptosis that contributed to the carcinogenesis. The purpose of this study is to explore the potential connection between high urinary urea concentration and the bladder disorders. METHODS: A high protein diet rat model was conducted by feeding with 40 % protein diet. In-silico modeling and algorithm, based on the results of microarray and proteomics from the bladder urothelium, were used for the reconstruction of accurate cellular networks and the identification of novel master regulators in the high-protein diet rat model. Pathway and biological process enrichment analysis were used to characterize predicted targets of candidate mRNAs/proteins. The expression pattern of the most significant master regulators was evaluated by qPCR and immunohistochemistry. RESULTS: Based on the analysis of different expressed mRNAs/proteins, 15 significant ones (CRP, MCPT2, MCPT9, EPXH2, SERPING1, SRGN, CDKN1C, CDK6, CCNB1, PCNA, BAX, MAGEB16, SERPINE1, HSPA2, FOS) were highly identified and verified by qPCR and immunohistochemistry. They were involved in immune and inflammatory response, cell cycle arrest, apoptosis and pathways in cancer. These abnormally activated processes caused the bladder interstitial congestion and inflammatory infiltrates under the thinner urothelium, cell desquamation, cytoplasm vacuolization, nucleus swelling and malformation in the high-protein diet group. CONCLUSIONS: We provided evidences that high urinary urea concentration caused by high-protein diet might be a potential carcinogenic factor in bladder. BioMed Central 2016-02-16 /pmc/articles/PMC4755000/ /pubmed/26879937 http://dx.doi.org/10.1186/s12967-016-0809-9 Text en © Liu et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Liu, Ming Li, Min Liu, Jiangfeng Wang, Hongkai Zhong, Dandan Zhou, Hong Yang, Baoxue Elevated urinary urea by high-protein diet could be one of the inducements of bladder disorders |
title | Elevated urinary urea by high-protein diet could be one of the inducements of bladder disorders |
title_full | Elevated urinary urea by high-protein diet could be one of the inducements of bladder disorders |
title_fullStr | Elevated urinary urea by high-protein diet could be one of the inducements of bladder disorders |
title_full_unstemmed | Elevated urinary urea by high-protein diet could be one of the inducements of bladder disorders |
title_short | Elevated urinary urea by high-protein diet could be one of the inducements of bladder disorders |
title_sort | elevated urinary urea by high-protein diet could be one of the inducements of bladder disorders |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4755000/ https://www.ncbi.nlm.nih.gov/pubmed/26879937 http://dx.doi.org/10.1186/s12967-016-0809-9 |
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