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EFFECT OF OMEGA-3 FATTY ACID IN THE HEALING PROCESS OF COLONIC ANASTOMOSIS IN RATS

BACKGROUND : The use of long-chain polyunsaturated fatty acids has been studied in the context of healing and tissue regeneration mainly due to its anti-inflammatory, immunoregulatory and antioncogenic properties. Previous studies have demonstrated beneficial effects with the use of enteral immunonu...

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Autores principales: de CASTILHO, Tiago Jacometo Coelho, CAMPOS, Antônio Carlos Ligocki, MELLO, Eneri Vieira de Souza Leite
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Colégio Brasileiro de Cirurgia Digestiva 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4755178/
https://www.ncbi.nlm.nih.gov/pubmed/26734796
http://dx.doi.org/10.1590/S0102-6720201500040010
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author de CASTILHO, Tiago Jacometo Coelho
CAMPOS, Antônio Carlos Ligocki
MELLO, Eneri Vieira de Souza Leite
author_facet de CASTILHO, Tiago Jacometo Coelho
CAMPOS, Antônio Carlos Ligocki
MELLO, Eneri Vieira de Souza Leite
author_sort de CASTILHO, Tiago Jacometo Coelho
collection PubMed
description BACKGROUND : The use of long-chain polyunsaturated fatty acids has been studied in the context of healing and tissue regeneration mainly due to its anti-inflammatory, immunoregulatory and antioncogenic properties. Previous studies have demonstrated beneficial effects with the use of enteral immunonutrition containing various farmaconutrients such as L-arginine, omega-3, trace elements, but the individual action of each component in the healing of colonic anastomosis remains unclear. AIM : To evaluate the influence of preoperative supplementation with omega-3 fatty acids on the healing of colonic anastomoses of well-nourished rats. METHODS : Forty Wistar adult male rats, weighing 234.4±22.3 g were used. The animals were divided into two groups: the control group received for seven days olive oil rich in omega-9 oil through an orogastric tube, while the study group received isocaloric and isovolumetric omega-3 emulsion at a dose of 100 mg/kg/day, also for seven days. Both groups were submitted to two colotomies followed by anastomosis, in the right and left colon, respectively. Parameters evaluated included changes in body weight, anastomotic complications and mortality, as well as maximum tensile strength by using a tensiometer and collagen densitometry at the anastomotic site. RESULTS : There were no differences in body weight or mortality and morbidity between groups. The value of the maximum tensile strength of the control group was 1.9±0.3 N and the study group 1.7±0.2, p=0.357. There was, however, a larger amount of type I collagen deposition in the study group (p=0.0126). The collagen maturation índex was 1.74±0.71 in the control group and 1.67±0.5 in the study group; p=0,719). CONCLUSIONS : Preoperative supplementation of omega-3 fatty acid in rats is associated with increased collagen deposition of type I fibers in colonic anastomoses on the 5(th) postoperative day. No differences were observed in the tensile strength or collagen maturation index.
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spelling pubmed-47551782016-02-24 EFFECT OF OMEGA-3 FATTY ACID IN THE HEALING PROCESS OF COLONIC ANASTOMOSIS IN RATS de CASTILHO, Tiago Jacometo Coelho CAMPOS, Antônio Carlos Ligocki MELLO, Eneri Vieira de Souza Leite Arq Bras Cir Dig Original Article BACKGROUND : The use of long-chain polyunsaturated fatty acids has been studied in the context of healing and tissue regeneration mainly due to its anti-inflammatory, immunoregulatory and antioncogenic properties. Previous studies have demonstrated beneficial effects with the use of enteral immunonutrition containing various farmaconutrients such as L-arginine, omega-3, trace elements, but the individual action of each component in the healing of colonic anastomosis remains unclear. AIM : To evaluate the influence of preoperative supplementation with omega-3 fatty acids on the healing of colonic anastomoses of well-nourished rats. METHODS : Forty Wistar adult male rats, weighing 234.4±22.3 g were used. The animals were divided into two groups: the control group received for seven days olive oil rich in omega-9 oil through an orogastric tube, while the study group received isocaloric and isovolumetric omega-3 emulsion at a dose of 100 mg/kg/day, also for seven days. Both groups were submitted to two colotomies followed by anastomosis, in the right and left colon, respectively. Parameters evaluated included changes in body weight, anastomotic complications and mortality, as well as maximum tensile strength by using a tensiometer and collagen densitometry at the anastomotic site. RESULTS : There were no differences in body weight or mortality and morbidity between groups. The value of the maximum tensile strength of the control group was 1.9±0.3 N and the study group 1.7±0.2, p=0.357. There was, however, a larger amount of type I collagen deposition in the study group (p=0.0126). The collagen maturation índex was 1.74±0.71 in the control group and 1.67±0.5 in the study group; p=0,719). CONCLUSIONS : Preoperative supplementation of omega-3 fatty acid in rats is associated with increased collagen deposition of type I fibers in colonic anastomoses on the 5(th) postoperative day. No differences were observed in the tensile strength or collagen maturation index. Colégio Brasileiro de Cirurgia Digestiva 2015 /pmc/articles/PMC4755178/ /pubmed/26734796 http://dx.doi.org/10.1590/S0102-6720201500040010 Text en http://creativecommons.org/licenses/by/4.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Original Article
de CASTILHO, Tiago Jacometo Coelho
CAMPOS, Antônio Carlos Ligocki
MELLO, Eneri Vieira de Souza Leite
EFFECT OF OMEGA-3 FATTY ACID IN THE HEALING PROCESS OF COLONIC ANASTOMOSIS IN RATS
title EFFECT OF OMEGA-3 FATTY ACID IN THE HEALING PROCESS OF COLONIC ANASTOMOSIS IN RATS
title_full EFFECT OF OMEGA-3 FATTY ACID IN THE HEALING PROCESS OF COLONIC ANASTOMOSIS IN RATS
title_fullStr EFFECT OF OMEGA-3 FATTY ACID IN THE HEALING PROCESS OF COLONIC ANASTOMOSIS IN RATS
title_full_unstemmed EFFECT OF OMEGA-3 FATTY ACID IN THE HEALING PROCESS OF COLONIC ANASTOMOSIS IN RATS
title_short EFFECT OF OMEGA-3 FATTY ACID IN THE HEALING PROCESS OF COLONIC ANASTOMOSIS IN RATS
title_sort effect of omega-3 fatty acid in the healing process of colonic anastomosis in rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4755178/
https://www.ncbi.nlm.nih.gov/pubmed/26734796
http://dx.doi.org/10.1590/S0102-6720201500040010
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