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Development of three-dimensional tissue engineered bone-oral mucosal composite models
Tissue engineering of bone and oral mucosa have been extensively studied independently. The aim of this study was to develop and investigate a novel combination of bone and oral mucosa in a single 3D in vitro composite tissue mimicking the natural structure of alveolar bone with an overlying oral mu...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756037/ https://www.ncbi.nlm.nih.gov/pubmed/26883949 http://dx.doi.org/10.1007/s10856-016-5676-7 |
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author | Almela, Thafar Brook, Ian M. Moharamzadeh, Keyvan |
author_facet | Almela, Thafar Brook, Ian M. Moharamzadeh, Keyvan |
author_sort | Almela, Thafar |
collection | PubMed |
description | Tissue engineering of bone and oral mucosa have been extensively studied independently. The aim of this study was to develop and investigate a novel combination of bone and oral mucosa in a single 3D in vitro composite tissue mimicking the natural structure of alveolar bone with an overlying oral mucosa. Rat osteosarcoma (ROS) cells were seeded into a hydroxyapatite/tri-calcium phosphate scaffold and bone constructs were cultured in a spinner bioreactor for 3 months. An engineered oral mucosa was fabricated by air/liquid interface culture of immortalized OKF6/TERET-2 oral keratinocytes on collagen gel-embedded fibroblasts. EOM was incorporated into the engineered bone using a tissue adhesive and further cultured prior to qualitative and quantitative assessments. Presto Blue assay revealed that ROS cells remained vital throughout the experiment. The histological and scanning electron microscope examinations showed that the cells proliferated and densely populated the scaffold construct. Micro computed tomography (micro-CT) scanning revealed an increase in closed porosity and a decrease in open and total porosity at the end of the culture period. Histological examination of bone-oral mucosa model showed a relatively differentiated parakeratinized epithelium, evenly distributed fibroblasts in the connective tissue layer and widely spread ROS cells within the bone scaffold. The feasibility of fabricating a novel bone-oral mucosa model using cell lines is demonstrated. Generating human ‘normal’ cell-based models with further characterization is required to optimize the model for in vitro and in vivo applications. |
format | Online Article Text |
id | pubmed-4756037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-47560372016-02-26 Development of three-dimensional tissue engineered bone-oral mucosal composite models Almela, Thafar Brook, Ian M. Moharamzadeh, Keyvan J Mater Sci Mater Med Tissue Engineering Constructs and Cell Substrates Tissue engineering of bone and oral mucosa have been extensively studied independently. The aim of this study was to develop and investigate a novel combination of bone and oral mucosa in a single 3D in vitro composite tissue mimicking the natural structure of alveolar bone with an overlying oral mucosa. Rat osteosarcoma (ROS) cells were seeded into a hydroxyapatite/tri-calcium phosphate scaffold and bone constructs were cultured in a spinner bioreactor for 3 months. An engineered oral mucosa was fabricated by air/liquid interface culture of immortalized OKF6/TERET-2 oral keratinocytes on collagen gel-embedded fibroblasts. EOM was incorporated into the engineered bone using a tissue adhesive and further cultured prior to qualitative and quantitative assessments. Presto Blue assay revealed that ROS cells remained vital throughout the experiment. The histological and scanning electron microscope examinations showed that the cells proliferated and densely populated the scaffold construct. Micro computed tomography (micro-CT) scanning revealed an increase in closed porosity and a decrease in open and total porosity at the end of the culture period. Histological examination of bone-oral mucosa model showed a relatively differentiated parakeratinized epithelium, evenly distributed fibroblasts in the connective tissue layer and widely spread ROS cells within the bone scaffold. The feasibility of fabricating a novel bone-oral mucosa model using cell lines is demonstrated. Generating human ‘normal’ cell-based models with further characterization is required to optimize the model for in vitro and in vivo applications. Springer US 2016-02-16 2016 /pmc/articles/PMC4756037/ /pubmed/26883949 http://dx.doi.org/10.1007/s10856-016-5676-7 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Tissue Engineering Constructs and Cell Substrates Almela, Thafar Brook, Ian M. Moharamzadeh, Keyvan Development of three-dimensional tissue engineered bone-oral mucosal composite models |
title | Development of three-dimensional tissue engineered bone-oral mucosal composite models |
title_full | Development of three-dimensional tissue engineered bone-oral mucosal composite models |
title_fullStr | Development of three-dimensional tissue engineered bone-oral mucosal composite models |
title_full_unstemmed | Development of three-dimensional tissue engineered bone-oral mucosal composite models |
title_short | Development of three-dimensional tissue engineered bone-oral mucosal composite models |
title_sort | development of three-dimensional tissue engineered bone-oral mucosal composite models |
topic | Tissue Engineering Constructs and Cell Substrates |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756037/ https://www.ncbi.nlm.nih.gov/pubmed/26883949 http://dx.doi.org/10.1007/s10856-016-5676-7 |
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