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Isolate Dependency of Brassica rapa Resistance QTLs to Botrytis cinerea
Generalist necrotrophic pathogens including Botrytis cinerea cause significant yield and financial losses on Brassica crops. However, there is little knowledge about the mechanisms underlying the complex interactions encoded by both host and pathogen genomes in this interaction. This potentially inc...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756292/ https://www.ncbi.nlm.nih.gov/pubmed/26925079 http://dx.doi.org/10.3389/fpls.2016.00161 |
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author | Zhang, Wei Kwon, Soon-Tae Chen, Fang Kliebenstein, Daniel J. |
author_facet | Zhang, Wei Kwon, Soon-Tae Chen, Fang Kliebenstein, Daniel J. |
author_sort | Zhang, Wei |
collection | PubMed |
description | Generalist necrotrophic pathogens including Botrytis cinerea cause significant yield and financial losses on Brassica crops. However, there is little knowledge about the mechanisms underlying the complex interactions encoded by both host and pathogen genomes in this interaction. This potentially includes multiple layers of plant defense and pathogen virulence mechanisms that could complicate in breeding broad spectrum resistance within Brassica species. Glucosinolates (GSLs) are a diverse group of defense metabolites that play a key role in interaction between Brassica and biotic attackers. In this study, we utilized a collection of diverse B. cinerea isolates to investigate resistance within the Brassica rapa R500 × IMB211 recombinant inbred line population. We tested variation on lesion development and glucosinolate accumulation in parental lines and all population lines. We then mapped quantitative trait loci (QTL) for both resistances to B. cinerea and defense metabolites in this population. Phenotypic analysis and QTL mapping demonstrate that the genetic basis of resistance to B. cinerea in B. rapa is isolate specific and polygenic with transgressive segregation that both parents contribute resistance alleles. QTLs controlling defensive GSLs are highly dependent on pathogen infection. An overlap of two QTLs identified between resistance to B. cinerea and defense metabolites also showed isolate specific effects. This work suggests that directly searching for resistance loci may not be the best approach at improving resistance in B. rapa to necrotrophic pathogen. |
format | Online Article Text |
id | pubmed-4756292 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47562922016-02-26 Isolate Dependency of Brassica rapa Resistance QTLs to Botrytis cinerea Zhang, Wei Kwon, Soon-Tae Chen, Fang Kliebenstein, Daniel J. Front Plant Sci Plant Science Generalist necrotrophic pathogens including Botrytis cinerea cause significant yield and financial losses on Brassica crops. However, there is little knowledge about the mechanisms underlying the complex interactions encoded by both host and pathogen genomes in this interaction. This potentially includes multiple layers of plant defense and pathogen virulence mechanisms that could complicate in breeding broad spectrum resistance within Brassica species. Glucosinolates (GSLs) are a diverse group of defense metabolites that play a key role in interaction between Brassica and biotic attackers. In this study, we utilized a collection of diverse B. cinerea isolates to investigate resistance within the Brassica rapa R500 × IMB211 recombinant inbred line population. We tested variation on lesion development and glucosinolate accumulation in parental lines and all population lines. We then mapped quantitative trait loci (QTL) for both resistances to B. cinerea and defense metabolites in this population. Phenotypic analysis and QTL mapping demonstrate that the genetic basis of resistance to B. cinerea in B. rapa is isolate specific and polygenic with transgressive segregation that both parents contribute resistance alleles. QTLs controlling defensive GSLs are highly dependent on pathogen infection. An overlap of two QTLs identified between resistance to B. cinerea and defense metabolites also showed isolate specific effects. This work suggests that directly searching for resistance loci may not be the best approach at improving resistance in B. rapa to necrotrophic pathogen. Frontiers Media S.A. 2016-02-17 /pmc/articles/PMC4756292/ /pubmed/26925079 http://dx.doi.org/10.3389/fpls.2016.00161 Text en Copyright © 2016 Zhang, Kwon, Chen and Kliebenstein. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Zhang, Wei Kwon, Soon-Tae Chen, Fang Kliebenstein, Daniel J. Isolate Dependency of Brassica rapa Resistance QTLs to Botrytis cinerea |
title | Isolate Dependency of Brassica rapa Resistance QTLs to Botrytis cinerea |
title_full | Isolate Dependency of Brassica rapa Resistance QTLs to Botrytis cinerea |
title_fullStr | Isolate Dependency of Brassica rapa Resistance QTLs to Botrytis cinerea |
title_full_unstemmed | Isolate Dependency of Brassica rapa Resistance QTLs to Botrytis cinerea |
title_short | Isolate Dependency of Brassica rapa Resistance QTLs to Botrytis cinerea |
title_sort | isolate dependency of brassica rapa resistance qtls to botrytis cinerea |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756292/ https://www.ncbi.nlm.nih.gov/pubmed/26925079 http://dx.doi.org/10.3389/fpls.2016.00161 |
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