Lattice filter for processing image data of three-dimensional protein nanocrystals

When 300 kV cryo-EM images at Scherzer focus are acquired from ∼100 nm thick three-dimensional protein nanocrystals using a Falcon 2 direct electron detector, Fourier transformation can reveal the crystalline lattice to surprisingly high resolutions, even though the images themselves seem to be devo...

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Detalles Bibliográficos
Autores principales: van Genderen, E., Li, Y.-W., Nederlof, I., Abrahams, J. P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2016
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756612/
https://www.ncbi.nlm.nih.gov/pubmed/26894532
http://dx.doi.org/10.1107/S205979831502149X
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author van Genderen, E.
Li, Y.-W.
Nederlof, I.
Abrahams, J. P.
author_facet van Genderen, E.
Li, Y.-W.
Nederlof, I.
Abrahams, J. P.
author_sort van Genderen, E.
collection PubMed
description When 300 kV cryo-EM images at Scherzer focus are acquired from ∼100 nm thick three-dimensional protein nanocrystals using a Falcon 2 direct electron detector, Fourier transformation can reveal the crystalline lattice to surprisingly high resolutions, even though the images themselves seem to be devoid of any contrast. Here, it is reported how this lattice information can be enhanced by means of a wave finder in combination with Wiener-type maximum-likelihood filtering. This procedure paves the way towards full three-dimensional structure determination at high resolution for protein crystals.
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spelling pubmed-47566122016-02-26 Lattice filter for processing image data of three-dimensional protein nanocrystals van Genderen, E. Li, Y.-W. Nederlof, I. Abrahams, J. P. Acta Crystallogr D Struct Biol Research Papers When 300 kV cryo-EM images at Scherzer focus are acquired from ∼100 nm thick three-dimensional protein nanocrystals using a Falcon 2 direct electron detector, Fourier transformation can reveal the crystalline lattice to surprisingly high resolutions, even though the images themselves seem to be devoid of any contrast. Here, it is reported how this lattice information can be enhanced by means of a wave finder in combination with Wiener-type maximum-likelihood filtering. This procedure paves the way towards full three-dimensional structure determination at high resolution for protein crystals. International Union of Crystallography 2016-01-01 /pmc/articles/PMC4756612/ /pubmed/26894532 http://dx.doi.org/10.1107/S205979831502149X Text en © van Genderen et al. 2016 http://creativecommons.org/licenses/by/2.0/uk/ This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
van Genderen, E.
Li, Y.-W.
Nederlof, I.
Abrahams, J. P.
Lattice filter for processing image data of three-dimensional protein nanocrystals
title Lattice filter for processing image data of three-dimensional protein nanocrystals
title_full Lattice filter for processing image data of three-dimensional protein nanocrystals
title_fullStr Lattice filter for processing image data of three-dimensional protein nanocrystals
title_full_unstemmed Lattice filter for processing image data of three-dimensional protein nanocrystals
title_short Lattice filter for processing image data of three-dimensional protein nanocrystals
title_sort lattice filter for processing image data of three-dimensional protein nanocrystals
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756612/
https://www.ncbi.nlm.nih.gov/pubmed/26894532
http://dx.doi.org/10.1107/S205979831502149X
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AT abrahamsjp latticefilterforprocessingimagedataofthreedimensionalproteinnanocrystals

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