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Design of a titering assay for lentiviral vectors utilizing direct extraction of DNA from transduced cells in microtiter plates

Using lentiviral vector products in clinical applications requires an accurate method for measuring transduction titer. For vectors lacking a marker gene, quantitative polymerase chain reaction is used to evaluate the number of vector DNA copies in transduced target cells, from which a transduction...

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Detalles Bibliográficos
Autores principales:	Murphy, Michele E, Vin, Chintan D, Slough, Megan M, Gombotz, Wayne R, Kelley-Clarke, Brenna
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756768/ https://www.ncbi.nlm.nih.gov/pubmed/26942209 http://dx.doi.org/10.1038/mtm.2016.5

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