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LOOP IIId of the HCV IRES is essential for the structural rearrangement of the 40S-HCV IRES complex
As obligatory intracellular parasites, viruses rely on cellular machines to complete their life cycle, and most importantly they recruit the host ribosomes to translate their mRNA. The Hepatitis C viral mRNA initiates translation by directly binding the 40S ribosomal subunit in such a way that the i...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756818/ https://www.ncbi.nlm.nih.gov/pubmed/26626152 http://dx.doi.org/10.1093/nar/gkv1325 |
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author | Angulo, Jenniffer Ulryck, Nathalie Deforges, Jules Chamond, Nathalie Lopez-Lastra, Marcelo Masquida, Benoît Sargueil, Bruno |
author_facet | Angulo, Jenniffer Ulryck, Nathalie Deforges, Jules Chamond, Nathalie Lopez-Lastra, Marcelo Masquida, Benoît Sargueil, Bruno |
author_sort | Angulo, Jenniffer |
collection | PubMed |
description | As obligatory intracellular parasites, viruses rely on cellular machines to complete their life cycle, and most importantly they recruit the host ribosomes to translate their mRNA. The Hepatitis C viral mRNA initiates translation by directly binding the 40S ribosomal subunit in such a way that the initiation codon is correctly positioned in the P site of the ribosome. Such a property is likely to be central for many viruses, therefore the description of host-pathogen interaction at the molecular level is instrumental to provide new therapeutic targets. In this study, we monitored the 40S ribosomal subunit and the viral RNA structural rearrangement induced upon the formation of the binary complex. We further took advantage of an IRES viral mutant mRNA deficient for translation to identify the interactions necessary to promote translation. Using a combination of structure probing in solution and molecular modeling we establish a whole atom model which appears to be very similar to the one obtained recently by cryoEM. Our model brings new information on the complex, and most importantly reveals some structural rearrangement within the ribosome. This study suggests that the formation of a ‘kissing complex’ between the viral RNA and the 18S ribosomal RNA locks the 40S ribosomal subunit in a conformation proficient for translation. |
format | Online Article Text |
id | pubmed-4756818 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-47568182016-02-18 LOOP IIId of the HCV IRES is essential for the structural rearrangement of the 40S-HCV IRES complex Angulo, Jenniffer Ulryck, Nathalie Deforges, Jules Chamond, Nathalie Lopez-Lastra, Marcelo Masquida, Benoît Sargueil, Bruno Nucleic Acids Res RNA As obligatory intracellular parasites, viruses rely on cellular machines to complete their life cycle, and most importantly they recruit the host ribosomes to translate their mRNA. The Hepatitis C viral mRNA initiates translation by directly binding the 40S ribosomal subunit in such a way that the initiation codon is correctly positioned in the P site of the ribosome. Such a property is likely to be central for many viruses, therefore the description of host-pathogen interaction at the molecular level is instrumental to provide new therapeutic targets. In this study, we monitored the 40S ribosomal subunit and the viral RNA structural rearrangement induced upon the formation of the binary complex. We further took advantage of an IRES viral mutant mRNA deficient for translation to identify the interactions necessary to promote translation. Using a combination of structure probing in solution and molecular modeling we establish a whole atom model which appears to be very similar to the one obtained recently by cryoEM. Our model brings new information on the complex, and most importantly reveals some structural rearrangement within the ribosome. This study suggests that the formation of a ‘kissing complex’ between the viral RNA and the 18S ribosomal RNA locks the 40S ribosomal subunit in a conformation proficient for translation. Oxford University Press 2016-02-18 2015-11-30 /pmc/articles/PMC4756818/ /pubmed/26626152 http://dx.doi.org/10.1093/nar/gkv1325 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | RNA Angulo, Jenniffer Ulryck, Nathalie Deforges, Jules Chamond, Nathalie Lopez-Lastra, Marcelo Masquida, Benoît Sargueil, Bruno LOOP IIId of the HCV IRES is essential for the structural rearrangement of the 40S-HCV IRES complex |
title | LOOP IIId of the HCV IRES is essential for the structural rearrangement of the 40S-HCV IRES complex |
title_full | LOOP IIId of the HCV IRES is essential for the structural rearrangement of the 40S-HCV IRES complex |
title_fullStr | LOOP IIId of the HCV IRES is essential for the structural rearrangement of the 40S-HCV IRES complex |
title_full_unstemmed | LOOP IIId of the HCV IRES is essential for the structural rearrangement of the 40S-HCV IRES complex |
title_short | LOOP IIId of the HCV IRES is essential for the structural rearrangement of the 40S-HCV IRES complex |
title_sort | loop iiid of the hcv ires is essential for the structural rearrangement of the 40s-hcv ires complex |
topic | RNA |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756818/ https://www.ncbi.nlm.nih.gov/pubmed/26626152 http://dx.doi.org/10.1093/nar/gkv1325 |
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