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DNA Subtraction of In Vivo Selected Phage Repertoires for Efficient Peptide Pathology Biomarker Identification in Neuroinflammation Multiple Sclerosis Model

To streamline in vivo biomarker discovery, we developed a suppression subtractive DNA hybridization technique adapted for phage-displayed combinatorial libraries of 12 amino acid peptides (PhiSSH). Physical DNA subtraction is performed in a one-tube-all-reactions format by sequential addition of rea...

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Detalles Bibliográficos
Autores principales: Vargas-Sanchez, Karina, Vekris, Antonios, Petry, Klaus G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Libertas Academica 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756861/
https://www.ncbi.nlm.nih.gov/pubmed/26917946
http://dx.doi.org/10.4137/BMI.S32188