Cargando…

Expression of Innate Immunity Genes in Epithelial Cells of Hypertrophic Adenoids with and without Pediatric Chronic Rhinosinusitis: A Preliminary Report

BACKGROUND: Adenoid hypertrophy (AH) is associated with pediatric chronic rhinosinusitis (pCRS), but its role in the inflammatory process of pCRS is unclear. It is thought that innate immunity gene expression is disrupted in the epithelium of patients with chronic rhinosinusitis (CRS), including ant...

Descripción completa

Detalles Bibliográficos
Autores principales: Qu, Xiao-Peng, Huang, Zhen-Xiao, Sun, Yan, Ye, Ting, Cui, Shun-Jiu, Huang, Qian, Ma, Li-Jing, Yang, Qing-Wen, Wang, Hong, Fan, Er-Zhong, Li, Ying, Zhang, Liang, Zhou, Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756897/
https://www.ncbi.nlm.nih.gov/pubmed/26521790
http://dx.doi.org/10.4103/0366-6999.168056
_version_ 1782416417991163904
author Qu, Xiao-Peng
Huang, Zhen-Xiao
Sun, Yan
Ye, Ting
Cui, Shun-Jiu
Huang, Qian
Ma, Li-Jing
Yang, Qing-Wen
Wang, Hong
Fan, Er-Zhong
Li, Ying
Zhang, Liang
Zhou, Bing
author_facet Qu, Xiao-Peng
Huang, Zhen-Xiao
Sun, Yan
Ye, Ting
Cui, Shun-Jiu
Huang, Qian
Ma, Li-Jing
Yang, Qing-Wen
Wang, Hong
Fan, Er-Zhong
Li, Ying
Zhang, Liang
Zhou, Bing
author_sort Qu, Xiao-Peng
collection PubMed
description BACKGROUND: Adenoid hypertrophy (AH) is associated with pediatric chronic rhinosinusitis (pCRS), but its role in the inflammatory process of pCRS is unclear. It is thought that innate immunity gene expression is disrupted in the epithelium of patients with chronic rhinosinusitis (CRS), including antimicrobial peptides and pattern recognition receptors (PRRs). The aim of this preliminary study was to detect the expression of innate immunity genes in epithelial cells of hypertrophic adenoids with and without pCRS to better understand their role in pCRS. METHODS: Nine pCRS patients and nine simple AH patients undergoing adenoidectomy were recruited for the study. Adenoidal epithelium was isolated, and real-time quantitative polymerase chain reaction (RT-qPCR) was employed to measure relative expression levels of the following messenger RNAs in hypertrophic adenoid epithelial cells of pediatric patients with and without CRS: Human β-defensin (HBD) 2 and 3, surfactant protein (SP)-A and D, toll-like receptors 1–10, nucleotide-binding oligomerization domain (NOD)-like receptors NOD 1, NOD 2, and NACHT, LRR and PYD domains-containing protein 3, retinoic acid-induced gene 1, melanoma differentiation-associated gene 5, and nuclear factor-κB (NF-κB). RT-qPCR data from two groups were analyzed by independent sample t-tests and Mann-Whitney U-tests. RESULTS: The relative expression of SP-D in adenoidal epithelium of pCRS group was significantly lower than that in AH group (pCRS 0.73 ± 0.10 vs. AH 1.21 ± 0.15; P = 0.0173, t = 2.654). The relative expression levels of all tested PRRs and NF-κB, as well as HBD-2, HBD-3, and SP-A, showed no statistically significant differences in isolated adenoidal epithelium between pCRS group and AH group. CONCLUSIONS: Down-regulated SP-D levels in adenoidal epithelium may contribute to the development of pCRS. PRRs, however, are unlikely to play a significant role in the inflammatory process of pCRS.
format Online
Article
Text
id pubmed-4756897
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Medknow Publications & Media Pvt Ltd
record_format MEDLINE/PubMed
spelling pubmed-47568972016-04-04 Expression of Innate Immunity Genes in Epithelial Cells of Hypertrophic Adenoids with and without Pediatric Chronic Rhinosinusitis: A Preliminary Report Qu, Xiao-Peng Huang, Zhen-Xiao Sun, Yan Ye, Ting Cui, Shun-Jiu Huang, Qian Ma, Li-Jing Yang, Qing-Wen Wang, Hong Fan, Er-Zhong Li, Ying Zhang, Liang Zhou, Bing Chin Med J (Engl) Original Article BACKGROUND: Adenoid hypertrophy (AH) is associated with pediatric chronic rhinosinusitis (pCRS), but its role in the inflammatory process of pCRS is unclear. It is thought that innate immunity gene expression is disrupted in the epithelium of patients with chronic rhinosinusitis (CRS), including antimicrobial peptides and pattern recognition receptors (PRRs). The aim of this preliminary study was to detect the expression of innate immunity genes in epithelial cells of hypertrophic adenoids with and without pCRS to better understand their role in pCRS. METHODS: Nine pCRS patients and nine simple AH patients undergoing adenoidectomy were recruited for the study. Adenoidal epithelium was isolated, and real-time quantitative polymerase chain reaction (RT-qPCR) was employed to measure relative expression levels of the following messenger RNAs in hypertrophic adenoid epithelial cells of pediatric patients with and without CRS: Human β-defensin (HBD) 2 and 3, surfactant protein (SP)-A and D, toll-like receptors 1–10, nucleotide-binding oligomerization domain (NOD)-like receptors NOD 1, NOD 2, and NACHT, LRR and PYD domains-containing protein 3, retinoic acid-induced gene 1, melanoma differentiation-associated gene 5, and nuclear factor-κB (NF-κB). RT-qPCR data from two groups were analyzed by independent sample t-tests and Mann-Whitney U-tests. RESULTS: The relative expression of SP-D in adenoidal epithelium of pCRS group was significantly lower than that in AH group (pCRS 0.73 ± 0.10 vs. AH 1.21 ± 0.15; P = 0.0173, t = 2.654). The relative expression levels of all tested PRRs and NF-κB, as well as HBD-2, HBD-3, and SP-A, showed no statistically significant differences in isolated adenoidal epithelium between pCRS group and AH group. CONCLUSIONS: Down-regulated SP-D levels in adenoidal epithelium may contribute to the development of pCRS. PRRs, however, are unlikely to play a significant role in the inflammatory process of pCRS. Medknow Publications & Media Pvt Ltd 2015-11-05 /pmc/articles/PMC4756897/ /pubmed/26521790 http://dx.doi.org/10.4103/0366-6999.168056 Text en Copyright: © 2015 Chinese Medical Journal http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Qu, Xiao-Peng
Huang, Zhen-Xiao
Sun, Yan
Ye, Ting
Cui, Shun-Jiu
Huang, Qian
Ma, Li-Jing
Yang, Qing-Wen
Wang, Hong
Fan, Er-Zhong
Li, Ying
Zhang, Liang
Zhou, Bing
Expression of Innate Immunity Genes in Epithelial Cells of Hypertrophic Adenoids with and without Pediatric Chronic Rhinosinusitis: A Preliminary Report
title Expression of Innate Immunity Genes in Epithelial Cells of Hypertrophic Adenoids with and without Pediatric Chronic Rhinosinusitis: A Preliminary Report
title_full Expression of Innate Immunity Genes in Epithelial Cells of Hypertrophic Adenoids with and without Pediatric Chronic Rhinosinusitis: A Preliminary Report
title_fullStr Expression of Innate Immunity Genes in Epithelial Cells of Hypertrophic Adenoids with and without Pediatric Chronic Rhinosinusitis: A Preliminary Report
title_full_unstemmed Expression of Innate Immunity Genes in Epithelial Cells of Hypertrophic Adenoids with and without Pediatric Chronic Rhinosinusitis: A Preliminary Report
title_short Expression of Innate Immunity Genes in Epithelial Cells of Hypertrophic Adenoids with and without Pediatric Chronic Rhinosinusitis: A Preliminary Report
title_sort expression of innate immunity genes in epithelial cells of hypertrophic adenoids with and without pediatric chronic rhinosinusitis: a preliminary report
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756897/
https://www.ncbi.nlm.nih.gov/pubmed/26521790
http://dx.doi.org/10.4103/0366-6999.168056
work_keys_str_mv AT quxiaopeng expressionofinnateimmunitygenesinepithelialcellsofhypertrophicadenoidswithandwithoutpediatricchronicrhinosinusitisapreliminaryreport
AT huangzhenxiao expressionofinnateimmunitygenesinepithelialcellsofhypertrophicadenoidswithandwithoutpediatricchronicrhinosinusitisapreliminaryreport
AT sunyan expressionofinnateimmunitygenesinepithelialcellsofhypertrophicadenoidswithandwithoutpediatricchronicrhinosinusitisapreliminaryreport
AT yeting expressionofinnateimmunitygenesinepithelialcellsofhypertrophicadenoidswithandwithoutpediatricchronicrhinosinusitisapreliminaryreport
AT cuishunjiu expressionofinnateimmunitygenesinepithelialcellsofhypertrophicadenoidswithandwithoutpediatricchronicrhinosinusitisapreliminaryreport
AT huangqian expressionofinnateimmunitygenesinepithelialcellsofhypertrophicadenoidswithandwithoutpediatricchronicrhinosinusitisapreliminaryreport
AT malijing expressionofinnateimmunitygenesinepithelialcellsofhypertrophicadenoidswithandwithoutpediatricchronicrhinosinusitisapreliminaryreport
AT yangqingwen expressionofinnateimmunitygenesinepithelialcellsofhypertrophicadenoidswithandwithoutpediatricchronicrhinosinusitisapreliminaryreport
AT wanghong expressionofinnateimmunitygenesinepithelialcellsofhypertrophicadenoidswithandwithoutpediatricchronicrhinosinusitisapreliminaryreport
AT fanerzhong expressionofinnateimmunitygenesinepithelialcellsofhypertrophicadenoidswithandwithoutpediatricchronicrhinosinusitisapreliminaryreport
AT liying expressionofinnateimmunitygenesinepithelialcellsofhypertrophicadenoidswithandwithoutpediatricchronicrhinosinusitisapreliminaryreport
AT zhangliang expressionofinnateimmunitygenesinepithelialcellsofhypertrophicadenoidswithandwithoutpediatricchronicrhinosinusitisapreliminaryreport
AT zhoubing expressionofinnateimmunitygenesinepithelialcellsofhypertrophicadenoidswithandwithoutpediatricchronicrhinosinusitisapreliminaryreport