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In vitro studies reveal antiurolithic effect of Terminalia arjuna using quantitative morphological information from computerized microscopy
PURPOSE: For most cases, urolithiasis is a condition where excessive oxalate is present in the urine. Many reports have documented free radical generation followed by hyperoxaluria as a consequence of which calcium oxalate (CaOx) deposition occurs in the kidney tissue. The present study is aimed to...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Urologia
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756970/ https://www.ncbi.nlm.nih.gov/pubmed/26689519 http://dx.doi.org/10.1590/S1677-5538.IBJU.2014.0547 |
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author | Mittal, A. Tandon, S. Singla, S.K. Tandon, C. |
author_facet | Mittal, A. Tandon, S. Singla, S.K. Tandon, C. |
author_sort | Mittal, A. |
collection | PubMed |
description | PURPOSE: For most cases, urolithiasis is a condition where excessive oxalate is present in the urine. Many reports have documented free radical generation followed by hyperoxaluria as a consequence of which calcium oxalate (CaOx) deposition occurs in the kidney tissue. The present study is aimed to exam the antilithiatic potency of the aqueous extract (AE) of Terminalia arjuna (T. arjuna). MATERIALS AND METHODS: The antilithiatic activity of Terminalia arjuna was investigated in vitro nucleation, aggregation and growth of the CaOx crystals as well as the morphology of CaOx crystals using the inbuilt software ‘Image-Pro Plus 7.0’ of Olympus upright microscope (BX53). Antioxidant activity of AE of Terminalia arjuna bark was also determined in vitro. RESULTS: Terminalia arjuna extract exhibited a concentration dependent inhibition of nucleation and aggregation of CaOx crystals. The AE of Terminalia arjuna bark also inhibited the growth of CaOx crystals. At the same time, the AE also modified the morphology of CaOx crystals from hexagonal to spherical shape with increasing concentrations of AE and reduced the dimensions such as area, perimeter, length and width of CaOx crystals in a dose dependent manner. Also, the Terminalia arjuna AE scavenged the DPPH (2, 2-diphenyl-1-picrylhydrazyl) radicals with an IC(50) at 13.1µg/mL. CONCLUSIONS: The study suggests that Terminalia arjuna bark has the potential to scavenge DPPH radicals and inhibit CaOx crystallization in vitro. In the light of these studies, Terminalia arjuna can be regarded as a promising candidate from natural plant sources of antilithiatic and antioxidant activity with high value. |
format | Online Article Text |
id | pubmed-4756970 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Sociedade Brasileira de Urologia |
record_format | MEDLINE/PubMed |
spelling | pubmed-47569702016-05-09 In vitro studies reveal antiurolithic effect of Terminalia arjuna using quantitative morphological information from computerized microscopy Mittal, A. Tandon, S. Singla, S.K. Tandon, C. Int Braz J Urol Original Article PURPOSE: For most cases, urolithiasis is a condition where excessive oxalate is present in the urine. Many reports have documented free radical generation followed by hyperoxaluria as a consequence of which calcium oxalate (CaOx) deposition occurs in the kidney tissue. The present study is aimed to exam the antilithiatic potency of the aqueous extract (AE) of Terminalia arjuna (T. arjuna). MATERIALS AND METHODS: The antilithiatic activity of Terminalia arjuna was investigated in vitro nucleation, aggregation and growth of the CaOx crystals as well as the morphology of CaOx crystals using the inbuilt software ‘Image-Pro Plus 7.0’ of Olympus upright microscope (BX53). Antioxidant activity of AE of Terminalia arjuna bark was also determined in vitro. RESULTS: Terminalia arjuna extract exhibited a concentration dependent inhibition of nucleation and aggregation of CaOx crystals. The AE of Terminalia arjuna bark also inhibited the growth of CaOx crystals. At the same time, the AE also modified the morphology of CaOx crystals from hexagonal to spherical shape with increasing concentrations of AE and reduced the dimensions such as area, perimeter, length and width of CaOx crystals in a dose dependent manner. Also, the Terminalia arjuna AE scavenged the DPPH (2, 2-diphenyl-1-picrylhydrazyl) radicals with an IC(50) at 13.1µg/mL. CONCLUSIONS: The study suggests that Terminalia arjuna bark has the potential to scavenge DPPH radicals and inhibit CaOx crystallization in vitro. In the light of these studies, Terminalia arjuna can be regarded as a promising candidate from natural plant sources of antilithiatic and antioxidant activity with high value. Sociedade Brasileira de Urologia 2015 /pmc/articles/PMC4756970/ /pubmed/26689519 http://dx.doi.org/10.1590/S1677-5538.IBJU.2014.0547 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Mittal, A. Tandon, S. Singla, S.K. Tandon, C. In vitro studies reveal antiurolithic effect of Terminalia arjuna using quantitative morphological information from computerized microscopy |
title |
In vitro studies reveal antiurolithic effect of Terminalia arjuna using quantitative morphological information from computerized microscopy |
title_full |
In vitro studies reveal antiurolithic effect of Terminalia arjuna using quantitative morphological information from computerized microscopy |
title_fullStr |
In vitro studies reveal antiurolithic effect of Terminalia arjuna using quantitative morphological information from computerized microscopy |
title_full_unstemmed |
In vitro studies reveal antiurolithic effect of Terminalia arjuna using quantitative morphological information from computerized microscopy |
title_short |
In vitro studies reveal antiurolithic effect of Terminalia arjuna using quantitative morphological information from computerized microscopy |
title_sort | in vitro studies reveal antiurolithic effect of terminalia arjuna using quantitative morphological information from computerized microscopy |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4756970/ https://www.ncbi.nlm.nih.gov/pubmed/26689519 http://dx.doi.org/10.1590/S1677-5538.IBJU.2014.0547 |
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