Mathematical Model of the Firefly Luciferase Complementation Assay Reveals a Non-Linear Relationship between the Detected Luminescence and the Affinity of the Protein Pair Being Analyzed

The firefly luciferase complementation assay is widely used as a bioluminescent reporter technology to detect protein-protein interactions in vitro, in cellulo, and in vivo. Upon the interaction of a protein pair, complemented firefly luciferase emits light through the adenylation and oxidation of i...

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Detalles Bibliográficos
Autores principales: Dale, Renee, Ohmuro-Matsuyama, Yuki, Ueda, Hiroshi, Kato, Naohiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757408/
https://www.ncbi.nlm.nih.gov/pubmed/26886551
http://dx.doi.org/10.1371/journal.pone.0148256

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757408/
https://www.ncbi.nlm.nih.gov/pubmed/26886551
http://dx.doi.org/10.1371/journal.pone.0148256

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