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Preservation of protein fluorescence in embedded human dendritic cells for targeted 3D light and electron microscopy

In this study, we present a correlative microscopy workflow to combine detailed 3D fluorescence light microscopy data with ultrastructural information gained by 3D focused ion beam assisted scanning electron microscopy. The workflow is based on an optimized high pressure freezing/freeze substitution...

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Detalles Bibliográficos
Autores principales:	HÖHN, K., FUCHS, J., FRÖBER, A., KIRMSE, R., GLASS, B., ANDERS‐ÖSSWEIN, M., WALTHER, P., KRÄUSSLICH, H.‐G., DIETRICH, C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2015
Materias:	Themed Issue Papers
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757415/ https://www.ncbi.nlm.nih.gov/pubmed/25786567 http://dx.doi.org/10.1111/jmi.12230

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