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Lychee flower extract inhibits proliferation and viral replication of HSV-1-infected corneal epithelial cells

PURPOSE: Herpes simplex virus type I (HSV-1) is capable of causing a wide array of human ocular diseases. Herpes simplex virus keratitis (HSK)-induced cytopathogenicity together with the chronic immune-inflammatory reaction can trigger stromal scarring, thinning, and neovascularization which may lea...

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Autores principales: Hsu, Chang-Min, Chiang, Samuel Tung-Hsing, Chang, Yuan-Yen, Chen, Yi-Chen, Yang, Deng-Jye, Chen, Ya-Yu, Lin, Hui-Wen, Tseng, Jung-Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Vision 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757453/
https://www.ncbi.nlm.nih.gov/pubmed/26937165
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author Hsu, Chang-Min
Chiang, Samuel Tung-Hsing
Chang, Yuan-Yen
Chen, Yi-Chen
Yang, Deng-Jye
Chen, Ya-Yu
Lin, Hui-Wen
Tseng, Jung-Kai
author_facet Hsu, Chang-Min
Chiang, Samuel Tung-Hsing
Chang, Yuan-Yen
Chen, Yi-Chen
Yang, Deng-Jye
Chen, Ya-Yu
Lin, Hui-Wen
Tseng, Jung-Kai
author_sort Hsu, Chang-Min
collection PubMed
description PURPOSE: Herpes simplex virus type I (HSV-1) is capable of causing a wide array of human ocular diseases. Herpes simplex virus keratitis (HSK)-induced cytopathogenicity together with the chronic immune-inflammatory reaction can trigger stromal scarring, thinning, and neovascularization which may lead to permanent vision impairment. Lychee flower extract (LFE) is known for its antioxidant and anti-inflammatory effects. Therefore, in this study, we investigated the mechanism of the Statens Seruminstitut rabbit corneal (SIRC) epithelial cells infected by HSV-1 and examined the antiviral capabilities of LFE. METHODS: SIRC cells were pretreated with different concentrations of LFE (0.2, 0.1, and 0.05 μg/ml) and then infected with 1 MOI of HSV-1 for 24 h. The cell viability or morphology was evaluated in this study. In addition, the supernatants and cell extracts were collected for Cell Counting Kit-8 (CCK), plaque assay, and western blotting. RESULTS: We found that HSV-1-induced cell proliferation is regulated through inhibition of the mammalian target of rapamycin (mTOR) and p70s6k phosphorylation in response to the LFE. In addition, the LFE enhanced the autophagy protein expression (Beclin-1 and light chain 3, LC3) and decreased the viral titers. CONCLUSIONS: These results showed the antiviral capabilities and the protective effects of LFE. Taken together, our data indicate that LFE has potential as an anti-HSK (herpes simplex keratitis) for HSV-1 infection.
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spelling pubmed-47574532016-03-02 Lychee flower extract inhibits proliferation and viral replication of HSV-1-infected corneal epithelial cells Hsu, Chang-Min Chiang, Samuel Tung-Hsing Chang, Yuan-Yen Chen, Yi-Chen Yang, Deng-Jye Chen, Ya-Yu Lin, Hui-Wen Tseng, Jung-Kai Mol Vis Research Article PURPOSE: Herpes simplex virus type I (HSV-1) is capable of causing a wide array of human ocular diseases. Herpes simplex virus keratitis (HSK)-induced cytopathogenicity together with the chronic immune-inflammatory reaction can trigger stromal scarring, thinning, and neovascularization which may lead to permanent vision impairment. Lychee flower extract (LFE) is known for its antioxidant and anti-inflammatory effects. Therefore, in this study, we investigated the mechanism of the Statens Seruminstitut rabbit corneal (SIRC) epithelial cells infected by HSV-1 and examined the antiviral capabilities of LFE. METHODS: SIRC cells were pretreated with different concentrations of LFE (0.2, 0.1, and 0.05 μg/ml) and then infected with 1 MOI of HSV-1 for 24 h. The cell viability or morphology was evaluated in this study. In addition, the supernatants and cell extracts were collected for Cell Counting Kit-8 (CCK), plaque assay, and western blotting. RESULTS: We found that HSV-1-induced cell proliferation is regulated through inhibition of the mammalian target of rapamycin (mTOR) and p70s6k phosphorylation in response to the LFE. In addition, the LFE enhanced the autophagy protein expression (Beclin-1 and light chain 3, LC3) and decreased the viral titers. CONCLUSIONS: These results showed the antiviral capabilities and the protective effects of LFE. Taken together, our data indicate that LFE has potential as an anti-HSK (herpes simplex keratitis) for HSV-1 infection. Molecular Vision 2016-02-10 /pmc/articles/PMC4757453/ /pubmed/26937165 Text en Copyright © 2016 Molecular Vision. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed.
spellingShingle Research Article
Hsu, Chang-Min
Chiang, Samuel Tung-Hsing
Chang, Yuan-Yen
Chen, Yi-Chen
Yang, Deng-Jye
Chen, Ya-Yu
Lin, Hui-Wen
Tseng, Jung-Kai
Lychee flower extract inhibits proliferation and viral replication of HSV-1-infected corneal epithelial cells
title Lychee flower extract inhibits proliferation and viral replication of HSV-1-infected corneal epithelial cells
title_full Lychee flower extract inhibits proliferation and viral replication of HSV-1-infected corneal epithelial cells
title_fullStr Lychee flower extract inhibits proliferation and viral replication of HSV-1-infected corneal epithelial cells
title_full_unstemmed Lychee flower extract inhibits proliferation and viral replication of HSV-1-infected corneal epithelial cells
title_short Lychee flower extract inhibits proliferation and viral replication of HSV-1-infected corneal epithelial cells
title_sort lychee flower extract inhibits proliferation and viral replication of hsv-1-infected corneal epithelial cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757453/
https://www.ncbi.nlm.nih.gov/pubmed/26937165
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