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Lychee flower extract inhibits proliferation and viral replication of HSV-1-infected corneal epithelial cells
PURPOSE: Herpes simplex virus type I (HSV-1) is capable of causing a wide array of human ocular diseases. Herpes simplex virus keratitis (HSK)-induced cytopathogenicity together with the chronic immune-inflammatory reaction can trigger stromal scarring, thinning, and neovascularization which may lea...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Vision
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757453/ https://www.ncbi.nlm.nih.gov/pubmed/26937165 |
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author | Hsu, Chang-Min Chiang, Samuel Tung-Hsing Chang, Yuan-Yen Chen, Yi-Chen Yang, Deng-Jye Chen, Ya-Yu Lin, Hui-Wen Tseng, Jung-Kai |
author_facet | Hsu, Chang-Min Chiang, Samuel Tung-Hsing Chang, Yuan-Yen Chen, Yi-Chen Yang, Deng-Jye Chen, Ya-Yu Lin, Hui-Wen Tseng, Jung-Kai |
author_sort | Hsu, Chang-Min |
collection | PubMed |
description | PURPOSE: Herpes simplex virus type I (HSV-1) is capable of causing a wide array of human ocular diseases. Herpes simplex virus keratitis (HSK)-induced cytopathogenicity together with the chronic immune-inflammatory reaction can trigger stromal scarring, thinning, and neovascularization which may lead to permanent vision impairment. Lychee flower extract (LFE) is known for its antioxidant and anti-inflammatory effects. Therefore, in this study, we investigated the mechanism of the Statens Seruminstitut rabbit corneal (SIRC) epithelial cells infected by HSV-1 and examined the antiviral capabilities of LFE. METHODS: SIRC cells were pretreated with different concentrations of LFE (0.2, 0.1, and 0.05 μg/ml) and then infected with 1 MOI of HSV-1 for 24 h. The cell viability or morphology was evaluated in this study. In addition, the supernatants and cell extracts were collected for Cell Counting Kit-8 (CCK), plaque assay, and western blotting. RESULTS: We found that HSV-1-induced cell proliferation is regulated through inhibition of the mammalian target of rapamycin (mTOR) and p70s6k phosphorylation in response to the LFE. In addition, the LFE enhanced the autophagy protein expression (Beclin-1 and light chain 3, LC3) and decreased the viral titers. CONCLUSIONS: These results showed the antiviral capabilities and the protective effects of LFE. Taken together, our data indicate that LFE has potential as an anti-HSK (herpes simplex keratitis) for HSV-1 infection. |
format | Online Article Text |
id | pubmed-4757453 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Molecular Vision |
record_format | MEDLINE/PubMed |
spelling | pubmed-47574532016-03-02 Lychee flower extract inhibits proliferation and viral replication of HSV-1-infected corneal epithelial cells Hsu, Chang-Min Chiang, Samuel Tung-Hsing Chang, Yuan-Yen Chen, Yi-Chen Yang, Deng-Jye Chen, Ya-Yu Lin, Hui-Wen Tseng, Jung-Kai Mol Vis Research Article PURPOSE: Herpes simplex virus type I (HSV-1) is capable of causing a wide array of human ocular diseases. Herpes simplex virus keratitis (HSK)-induced cytopathogenicity together with the chronic immune-inflammatory reaction can trigger stromal scarring, thinning, and neovascularization which may lead to permanent vision impairment. Lychee flower extract (LFE) is known for its antioxidant and anti-inflammatory effects. Therefore, in this study, we investigated the mechanism of the Statens Seruminstitut rabbit corneal (SIRC) epithelial cells infected by HSV-1 and examined the antiviral capabilities of LFE. METHODS: SIRC cells were pretreated with different concentrations of LFE (0.2, 0.1, and 0.05 μg/ml) and then infected with 1 MOI of HSV-1 for 24 h. The cell viability or morphology was evaluated in this study. In addition, the supernatants and cell extracts were collected for Cell Counting Kit-8 (CCK), plaque assay, and western blotting. RESULTS: We found that HSV-1-induced cell proliferation is regulated through inhibition of the mammalian target of rapamycin (mTOR) and p70s6k phosphorylation in response to the LFE. In addition, the LFE enhanced the autophagy protein expression (Beclin-1 and light chain 3, LC3) and decreased the viral titers. CONCLUSIONS: These results showed the antiviral capabilities and the protective effects of LFE. Taken together, our data indicate that LFE has potential as an anti-HSK (herpes simplex keratitis) for HSV-1 infection. Molecular Vision 2016-02-10 /pmc/articles/PMC4757453/ /pubmed/26937165 Text en Copyright © 2016 Molecular Vision. http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited, used for non-commercial purposes, and is not altered or transformed. |
spellingShingle | Research Article Hsu, Chang-Min Chiang, Samuel Tung-Hsing Chang, Yuan-Yen Chen, Yi-Chen Yang, Deng-Jye Chen, Ya-Yu Lin, Hui-Wen Tseng, Jung-Kai Lychee flower extract inhibits proliferation and viral replication of HSV-1-infected corneal epithelial cells |
title | Lychee flower extract inhibits proliferation and viral replication of HSV-1-infected corneal epithelial cells |
title_full | Lychee flower extract inhibits proliferation and viral replication of HSV-1-infected corneal epithelial cells |
title_fullStr | Lychee flower extract inhibits proliferation and viral replication of HSV-1-infected corneal epithelial cells |
title_full_unstemmed | Lychee flower extract inhibits proliferation and viral replication of HSV-1-infected corneal epithelial cells |
title_short | Lychee flower extract inhibits proliferation and viral replication of HSV-1-infected corneal epithelial cells |
title_sort | lychee flower extract inhibits proliferation and viral replication of hsv-1-infected corneal epithelial cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757453/ https://www.ncbi.nlm.nih.gov/pubmed/26937165 |
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