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Development of a rapid, low-cost protoplast transfection system for switchgrass (Panicum virgatum L.)

KEY MESSAGE: A switchgrass protoplast system was developed, achieving a cost reduction of ~1000-fold, a threefold increase in transformation efficiency, and a fourfold reduction in required DNA quantity compared to previous methods. ABSTRACT: In recent years, there has been a resurgence in the use o...

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Detalles Bibliográficos
Autores principales: Burris, Kellie P., Dlugosz, Elizabeth M., Collins, A. Grace, Stewart, C. Neal, Lenaghan, Scott C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757626/
https://www.ncbi.nlm.nih.gov/pubmed/26685665
http://dx.doi.org/10.1007/s00299-015-1913-7