Generation of KCL021 research grade human embryonic stem cell line carrying a ΔF508 mutation in the CFTR gene

The KCL021 human embryonic stem cell line was derived from an embryo donated for research that carried a ΔF508 mutation affecting the CFTR gene encoding the cystic fibrosis transmembrane conductance regulator. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fi...

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Autores principales: Miere, Cristian, Hewitson, Heema, Wood, Victoria, Kadeva, Neli, Cornwell, Glenda, Codognotto, Stefano, Stephenson, Emma, Ilic, Dusko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Lab resource: Stem cell line
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757720/
https://www.ncbi.nlm.nih.gov/pubmed/27345808
http://dx.doi.org/10.1016/j.scr.2015.12.042
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author Miere, Cristian
Hewitson, Heema
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
author_facet Miere, Cristian
Hewitson, Heema
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
author_sort Miere, Cristian
collection PubMed
description The KCL021 human embryonic stem cell line was derived from an embryo donated for research that carried a ΔF508 mutation affecting the CFTR gene encoding the cystic fibrosis transmembrane conductance regulator. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays.
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spelling pubmed-47577202016-03-02 Generation of KCL021 research grade human embryonic stem cell line carrying a ΔF508 mutation in the CFTR gene Miere, Cristian Hewitson, Heema Wood, Victoria Kadeva, Neli Cornwell, Glenda Codognotto, Stefano Stephenson, Emma Ilic, Dusko Stem Cell Res Lab resource: Stem cell line The KCL021 human embryonic stem cell line was derived from an embryo donated for research that carried a ΔF508 mutation affecting the CFTR gene encoding the cystic fibrosis transmembrane conductance regulator. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro assays. Elsevier 2016-01 /pmc/articles/PMC4757720/ /pubmed/27345808 http://dx.doi.org/10.1016/j.scr.2015.12.042 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Lab resource: Stem cell line
Miere, Cristian
Hewitson, Heema
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
Generation of KCL021 research grade human embryonic stem cell line carrying a ΔF508 mutation in the CFTR gene
title Generation of KCL021 research grade human embryonic stem cell line carrying a ΔF508 mutation in the CFTR gene
title_full Generation of KCL021 research grade human embryonic stem cell line carrying a ΔF508 mutation in the CFTR gene
title_fullStr Generation of KCL021 research grade human embryonic stem cell line carrying a ΔF508 mutation in the CFTR gene
title_full_unstemmed Generation of KCL021 research grade human embryonic stem cell line carrying a ΔF508 mutation in the CFTR gene
title_short Generation of KCL021 research grade human embryonic stem cell line carrying a ΔF508 mutation in the CFTR gene
title_sort generation of kcl021 research grade human embryonic stem cell line carrying a δf508 mutation in the cftr gene
topic Lab resource: Stem cell line
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757720/
https://www.ncbi.nlm.nih.gov/pubmed/27345808
http://dx.doi.org/10.1016/j.scr.2015.12.042
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