Generation of KCL031 clinical grade human embryonic stem cell line

The KCL031 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free env...

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Autores principales: Jacquet, Laureen, Wood, Victoria, Kadeva, Neli, Cornwell, Glenda, Codognotto, Stefano, Hobbs, Carl, Stephenson, Emma, Ilic, Dusko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Lab resource: Stem cell line
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757722/
https://www.ncbi.nlm.nih.gov/pubmed/27345813
http://dx.doi.org/10.1016/j.scr.2015.12.033
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author Jacquet, Laureen
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Hobbs, Carl
Stephenson, Emma
Ilic, Dusko
author_facet Jacquet, Laureen
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Hobbs, Carl
Stephenson, Emma
Ilic, Dusko
author_sort Jacquet, Laureen
collection PubMed
description The KCL031 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays.
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spelling pubmed-47577222016-03-02 Generation of KCL031 clinical grade human embryonic stem cell line Jacquet, Laureen Wood, Victoria Kadeva, Neli Cornwell, Glenda Codognotto, Stefano Hobbs, Carl Stephenson, Emma Ilic, Dusko Stem Cell Res Lab resource: Stem cell line The KCL031 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays. Elsevier 2016-01 /pmc/articles/PMC4757722/ /pubmed/27345813 http://dx.doi.org/10.1016/j.scr.2015.12.033 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Lab resource: Stem cell line
Jacquet, Laureen
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Hobbs, Carl
Stephenson, Emma
Ilic, Dusko
Generation of KCL031 clinical grade human embryonic stem cell line
title Generation of KCL031 clinical grade human embryonic stem cell line
title_full Generation of KCL031 clinical grade human embryonic stem cell line
title_fullStr Generation of KCL031 clinical grade human embryonic stem cell line
title_full_unstemmed Generation of KCL031 clinical grade human embryonic stem cell line
title_short Generation of KCL031 clinical grade human embryonic stem cell line
title_sort generation of kcl031 clinical grade human embryonic stem cell line
topic Lab resource: Stem cell line
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757722/
https://www.ncbi.nlm.nih.gov/pubmed/27345813
http://dx.doi.org/10.1016/j.scr.2015.12.033
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