Generation of KCL032 clinical grade human embryonic stem cell line

The KCL032 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free env...

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Autores principales: Miere, Cristian, Wood, Victoria, Kadeva, Neli, Cornwell, Glenda, Codognotto, Stefano, Stephenson, Emma, Ilic, Dusko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Lab resource: Stem cell line
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757725/
https://www.ncbi.nlm.nih.gov/pubmed/27345778
http://dx.doi.org/10.1016/j.scr.2015.11.007
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author Miere, Cristian
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
author_facet Miere, Cristian
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
author_sort Miere, Cristian
collection PubMed
description The KCL032 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays.
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spelling pubmed-47577252016-03-02 Generation of KCL032 clinical grade human embryonic stem cell line Miere, Cristian Wood, Victoria Kadeva, Neli Cornwell, Glenda Codognotto, Stefano Stephenson, Emma Ilic, Dusko Stem Cell Res Lab resource: Stem cell line The KCL032 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. Elsevier 2016-01 /pmc/articles/PMC4757725/ /pubmed/27345778 http://dx.doi.org/10.1016/j.scr.2015.11.007 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Lab resource: Stem cell line
Miere, Cristian
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
Generation of KCL032 clinical grade human embryonic stem cell line
title Generation of KCL032 clinical grade human embryonic stem cell line
title_full Generation of KCL032 clinical grade human embryonic stem cell line
title_fullStr Generation of KCL032 clinical grade human embryonic stem cell line
title_full_unstemmed Generation of KCL032 clinical grade human embryonic stem cell line
title_short Generation of KCL032 clinical grade human embryonic stem cell line
title_sort generation of kcl032 clinical grade human embryonic stem cell line
topic Lab resource: Stem cell line
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757725/
https://www.ncbi.nlm.nih.gov/pubmed/27345778
http://dx.doi.org/10.1016/j.scr.2015.11.007
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