Generation of KCL038 clinical grade human embryonic stem cell line

The KCL038 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free env...

Descripción completa

Detalles Bibliográficos
Autores principales: Miere, Cristian, Wood, Victoria, Kadeva, Neli, Cornwell, Glenda, Codognotto, Stefano, Stephenson, Emma, Ilic, Dusko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Lab Resource: Stem Cell Line
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757727/
https://www.ncbi.nlm.nih.gov/pubmed/27345799
http://dx.doi.org/10.1016/j.scr.2015.12.024
_version_ 1782416499550453760
author Miere, Cristian
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
author_facet Miere, Cristian
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
author_sort Miere, Cristian
collection PubMed
description The KCL038 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays.
format Online
Article
Text
id pubmed-4757727
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-47577272016-03-02 Generation of KCL038 clinical grade human embryonic stem cell line Miere, Cristian Wood, Victoria Kadeva, Neli Cornwell, Glenda Codognotto, Stefano Stephenson, Emma Ilic, Dusko Stem Cell Res Lab Resource: Stem Cell Line The KCL038 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. Elsevier 2016-01 /pmc/articles/PMC4757727/ /pubmed/27345799 http://dx.doi.org/10.1016/j.scr.2015.12.024 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Lab Resource: Stem Cell Line
Miere, Cristian
Wood, Victoria
Kadeva, Neli
Cornwell, Glenda
Codognotto, Stefano
Stephenson, Emma
Ilic, Dusko
Generation of KCL038 clinical grade human embryonic stem cell line
title Generation of KCL038 clinical grade human embryonic stem cell line
title_full Generation of KCL038 clinical grade human embryonic stem cell line
title_fullStr Generation of KCL038 clinical grade human embryonic stem cell line
title_full_unstemmed Generation of KCL038 clinical grade human embryonic stem cell line
title_short Generation of KCL038 clinical grade human embryonic stem cell line
title_sort generation of kcl038 clinical grade human embryonic stem cell line
topic Lab Resource: Stem Cell Line
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757727/
https://www.ncbi.nlm.nih.gov/pubmed/27345799
http://dx.doi.org/10.1016/j.scr.2015.12.024
work_keys_str_mv AT mierecristian generationofkcl038clinicalgradehumanembryonicstemcellline
AT woodvictoria generationofkcl038clinicalgradehumanembryonicstemcellline
AT kadevaneli generationofkcl038clinicalgradehumanembryonicstemcellline
AT cornwellglenda generationofkcl038clinicalgradehumanembryonicstemcellline
AT codognottostefano generationofkcl038clinicalgradehumanembryonicstemcellline
AT stephensonemma generationofkcl038clinicalgradehumanembryonicstemcellline
AT ilicdusko generationofkcl038clinicalgradehumanembryonicstemcellline

Ejemplares similares