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Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus
In mammals, lipid droplets (LDs) are ubiquitous organelles that modulate immune and inflammatory responses through the production of lipid mediators. In insects, it is unknown whether LDs play any role during the development of immune responses. We show that Aedes aegypti Aag2 cells – an immune resp...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757862/ https://www.ncbi.nlm.nih.gov/pubmed/26887863 http://dx.doi.org/10.1038/srep19928 |
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author | Barletta, Ana Beatriz Ferreira Alves, Liliane Rosa Nascimento Silva, Maria Clara L. Sim, Shuzhen Dimopoulos, George Liechocki, Sally Maya-Monteiro, Clarissa M. Sorgine, Marcos H. Ferreira |
author_facet | Barletta, Ana Beatriz Ferreira Alves, Liliane Rosa Nascimento Silva, Maria Clara L. Sim, Shuzhen Dimopoulos, George Liechocki, Sally Maya-Monteiro, Clarissa M. Sorgine, Marcos H. Ferreira |
author_sort | Barletta, Ana Beatriz Ferreira |
collection | PubMed |
description | In mammals, lipid droplets (LDs) are ubiquitous organelles that modulate immune and inflammatory responses through the production of lipid mediators. In insects, it is unknown whether LDs play any role during the development of immune responses. We show that Aedes aegypti Aag2 cells – an immune responsive cell lineage – accumulates LDs when challenged with Enterobacter cloacae, Sindbis, and Dengue viruses. Microarray analysis of Aag2 challenged with E.cloacae or infected with Dengue virus revealed high transcripts levels of genes associated with lipid storage and LDs biogenesis, correlating with the increased LDs numbers in those conditions. Similarly, in mosquitoes, LDs accumulate in midgut cells in response to Serratia marcescens and Sindbis virus or when the native microbiota proliferates, following a blood meal. Also, constitutive activation of Toll and IMD pathways by knocking-down their respective negative modulators (Cactus and Caspar) increases LDs numbers in the midgut. Our results show for the first time an infection-induced LDs accumulation in response to both bacterial and viral infections in Ae. Aegypti, and we propose a role for LDs in mosquito immunity. These findings open new venues for further studies in insect immune responses associated with lipid metabolism. |
format | Online Article Text |
id | pubmed-4757862 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47578622016-02-26 Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus Barletta, Ana Beatriz Ferreira Alves, Liliane Rosa Nascimento Silva, Maria Clara L. Sim, Shuzhen Dimopoulos, George Liechocki, Sally Maya-Monteiro, Clarissa M. Sorgine, Marcos H. Ferreira Sci Rep Article In mammals, lipid droplets (LDs) are ubiquitous organelles that modulate immune and inflammatory responses through the production of lipid mediators. In insects, it is unknown whether LDs play any role during the development of immune responses. We show that Aedes aegypti Aag2 cells – an immune responsive cell lineage – accumulates LDs when challenged with Enterobacter cloacae, Sindbis, and Dengue viruses. Microarray analysis of Aag2 challenged with E.cloacae or infected with Dengue virus revealed high transcripts levels of genes associated with lipid storage and LDs biogenesis, correlating with the increased LDs numbers in those conditions. Similarly, in mosquitoes, LDs accumulate in midgut cells in response to Serratia marcescens and Sindbis virus or when the native microbiota proliferates, following a blood meal. Also, constitutive activation of Toll and IMD pathways by knocking-down their respective negative modulators (Cactus and Caspar) increases LDs numbers in the midgut. Our results show for the first time an infection-induced LDs accumulation in response to both bacterial and viral infections in Ae. Aegypti, and we propose a role for LDs in mosquito immunity. These findings open new venues for further studies in insect immune responses associated with lipid metabolism. Nature Publishing Group 2016-02-18 /pmc/articles/PMC4757862/ /pubmed/26887863 http://dx.doi.org/10.1038/srep19928 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Barletta, Ana Beatriz Ferreira Alves, Liliane Rosa Nascimento Silva, Maria Clara L. Sim, Shuzhen Dimopoulos, George Liechocki, Sally Maya-Monteiro, Clarissa M. Sorgine, Marcos H. Ferreira Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus |
title | Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus |
title_full | Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus |
title_fullStr | Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus |
title_full_unstemmed | Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus |
title_short | Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus |
title_sort | emerging role of lipid droplets in aedes aegypti immune response against bacteria and dengue virus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757862/ https://www.ncbi.nlm.nih.gov/pubmed/26887863 http://dx.doi.org/10.1038/srep19928 |
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