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Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus

In mammals, lipid droplets (LDs) are ubiquitous organelles that modulate immune and inflammatory responses through the production of lipid mediators. In insects, it is unknown whether LDs play any role during the development of immune responses. We show that Aedes aegypti Aag2 cells – an immune resp...

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Autores principales: Barletta, Ana Beatriz Ferreira, Alves, Liliane Rosa, Nascimento Silva, Maria Clara L., Sim, Shuzhen, Dimopoulos, George, Liechocki, Sally, Maya-Monteiro, Clarissa M., Sorgine, Marcos H. Ferreira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757862/
https://www.ncbi.nlm.nih.gov/pubmed/26887863
http://dx.doi.org/10.1038/srep19928
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author Barletta, Ana Beatriz Ferreira
Alves, Liliane Rosa
Nascimento Silva, Maria Clara L.
Sim, Shuzhen
Dimopoulos, George
Liechocki, Sally
Maya-Monteiro, Clarissa M.
Sorgine, Marcos H. Ferreira
author_facet Barletta, Ana Beatriz Ferreira
Alves, Liliane Rosa
Nascimento Silva, Maria Clara L.
Sim, Shuzhen
Dimopoulos, George
Liechocki, Sally
Maya-Monteiro, Clarissa M.
Sorgine, Marcos H. Ferreira
author_sort Barletta, Ana Beatriz Ferreira
collection PubMed
description In mammals, lipid droplets (LDs) are ubiquitous organelles that modulate immune and inflammatory responses through the production of lipid mediators. In insects, it is unknown whether LDs play any role during the development of immune responses. We show that Aedes aegypti Aag2 cells – an immune responsive cell lineage – accumulates LDs when challenged with Enterobacter cloacae, Sindbis, and Dengue viruses. Microarray analysis of Aag2 challenged with E.cloacae or infected with Dengue virus revealed high transcripts levels of genes associated with lipid storage and LDs biogenesis, correlating with the increased LDs numbers in those conditions. Similarly, in mosquitoes, LDs accumulate in midgut cells in response to Serratia marcescens and Sindbis virus or when the native microbiota proliferates, following a blood meal. Also, constitutive activation of Toll and IMD pathways by knocking-down their respective negative modulators (Cactus and Caspar) increases LDs numbers in the midgut. Our results show for the first time an infection-induced LDs accumulation in response to both bacterial and viral infections in Ae. Aegypti, and we propose a role for LDs in mosquito immunity. These findings open new venues for further studies in insect immune responses associated with lipid metabolism.
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spelling pubmed-47578622016-02-26 Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus Barletta, Ana Beatriz Ferreira Alves, Liliane Rosa Nascimento Silva, Maria Clara L. Sim, Shuzhen Dimopoulos, George Liechocki, Sally Maya-Monteiro, Clarissa M. Sorgine, Marcos H. Ferreira Sci Rep Article In mammals, lipid droplets (LDs) are ubiquitous organelles that modulate immune and inflammatory responses through the production of lipid mediators. In insects, it is unknown whether LDs play any role during the development of immune responses. We show that Aedes aegypti Aag2 cells – an immune responsive cell lineage – accumulates LDs when challenged with Enterobacter cloacae, Sindbis, and Dengue viruses. Microarray analysis of Aag2 challenged with E.cloacae or infected with Dengue virus revealed high transcripts levels of genes associated with lipid storage and LDs biogenesis, correlating with the increased LDs numbers in those conditions. Similarly, in mosquitoes, LDs accumulate in midgut cells in response to Serratia marcescens and Sindbis virus or when the native microbiota proliferates, following a blood meal. Also, constitutive activation of Toll and IMD pathways by knocking-down their respective negative modulators (Cactus and Caspar) increases LDs numbers in the midgut. Our results show for the first time an infection-induced LDs accumulation in response to both bacterial and viral infections in Ae. Aegypti, and we propose a role for LDs in mosquito immunity. These findings open new venues for further studies in insect immune responses associated with lipid metabolism. Nature Publishing Group 2016-02-18 /pmc/articles/PMC4757862/ /pubmed/26887863 http://dx.doi.org/10.1038/srep19928 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Barletta, Ana Beatriz Ferreira
Alves, Liliane Rosa
Nascimento Silva, Maria Clara L.
Sim, Shuzhen
Dimopoulos, George
Liechocki, Sally
Maya-Monteiro, Clarissa M.
Sorgine, Marcos H. Ferreira
Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus
title Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus
title_full Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus
title_fullStr Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus
title_full_unstemmed Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus
title_short Emerging role of lipid droplets in Aedes aegypti immune response against bacteria and Dengue virus
title_sort emerging role of lipid droplets in aedes aegypti immune response against bacteria and dengue virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757862/
https://www.ncbi.nlm.nih.gov/pubmed/26887863
http://dx.doi.org/10.1038/srep19928
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