Enrichment of G2/M cell cycle phase in human pluripotent stem cells enhances HDR-mediated gene repair with customizable endonucleases

Efficient gene editing is essential to fully utilize human pluripotent stem cells (hPSCs) in regenerative medicine. Custom endonuclease-based gene targeting involves two mechanisms of DNA repair: homology directed repair (HDR) and non-homologous end joining (NHEJ). HDR is the preferred mechanism for...

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Detalles Bibliográficos
Autores principales: Yang, Diane, Scavuzzo, Marissa A, Chmielowiec, Jolanta, Sharp, Robert, Bajic, Aleksandar, Borowiak, Malgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4757933/
https://www.ncbi.nlm.nih.gov/pubmed/26887909
http://dx.doi.org/10.1038/srep21264

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