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Forkhead box C1 induces epithelial-mesenchymal transition and is a potential therapeutic target in nasopharyngeal carcinoma

Nasopharyngeal carcinoma (NPC) is a highly invasive malignancy with cervical lymphopathy as the initial presentation. Epithelial-mesenchymal transition (EMT), a process by which epithelial cells lose cell-cell adhesion and gain migratory and invasive properties, has a pivotal role in metastasis. For...

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Autores principales: OU-YANG, LEI, XIAO, SHENG-JUN, LIU, PENG, YI, SHI-JANG, ZHANG, XIAO-LING, OU-YANG, SHI, TAN, SHENG-KUI, LEI, XUN
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4758279/
https://www.ncbi.nlm.nih.gov/pubmed/26461269
http://dx.doi.org/10.3892/mmr.2015.4427
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author OU-YANG, LEI
XIAO, SHENG-JUN
LIU, PENG
YI, SHI-JANG
ZHANG, XIAO-LING
OU-YANG, SHI
TAN, SHENG-KUI
LEI, XUN
author_facet OU-YANG, LEI
XIAO, SHENG-JUN
LIU, PENG
YI, SHI-JANG
ZHANG, XIAO-LING
OU-YANG, SHI
TAN, SHENG-KUI
LEI, XUN
author_sort OU-YANG, LEI
collection PubMed
description Nasopharyngeal carcinoma (NPC) is a highly invasive malignancy with cervical lymphopathy as the initial presentation. Epithelial-mesenchymal transition (EMT), a process by which epithelial cells lose cell-cell adhesion and gain migratory and invasive properties, has a pivotal role in metastasis. Forkhead box C1 (FoxC1), a member of the forkhead family of transcription factors, induces EMT and has a critical role in metastasis of multiple human cancers. However, the role of FoxC1 in the progression of NPC has remained elusive. The present study revealed that the expression of FoxC1 was markedly elevated in NPC tissues compared with that in chronically inflamed nasopharyngeal tissues and was closely correlated with vimentin, fibronectin and N-cadherin expression as indicated by immunohisto-chemical assays. In addition, high FoxC1 expression was positively associated with lymph node metastasis, distant metastasis and an advanced clinical stage in patients with NPC. Furthermore, FoxC1 expression was high in NPC cell lines while being low in an immortalized normal nasopharyngeal epithelial cell line. In vitro, knockdown of FoxC1 in the CNE2 human NPC cell line by small interfering RNA downregulated vimentin, fibronectin and N-cadherin expression and reduced the migratory and invasive capacity of CNE2 cells. In conclusion, the present study indicated that FoxC1 has a pivotal role in EMT through the upregulation of vimentin, fibronectin and N-cadherin expression. Thus, FoxC1 may be a potential therapeutic target in NPC.
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spelling pubmed-47582792016-03-04 Forkhead box C1 induces epithelial-mesenchymal transition and is a potential therapeutic target in nasopharyngeal carcinoma OU-YANG, LEI XIAO, SHENG-JUN LIU, PENG YI, SHI-JANG ZHANG, XIAO-LING OU-YANG, SHI TAN, SHENG-KUI LEI, XUN Mol Med Rep Articles Nasopharyngeal carcinoma (NPC) is a highly invasive malignancy with cervical lymphopathy as the initial presentation. Epithelial-mesenchymal transition (EMT), a process by which epithelial cells lose cell-cell adhesion and gain migratory and invasive properties, has a pivotal role in metastasis. Forkhead box C1 (FoxC1), a member of the forkhead family of transcription factors, induces EMT and has a critical role in metastasis of multiple human cancers. However, the role of FoxC1 in the progression of NPC has remained elusive. The present study revealed that the expression of FoxC1 was markedly elevated in NPC tissues compared with that in chronically inflamed nasopharyngeal tissues and was closely correlated with vimentin, fibronectin and N-cadherin expression as indicated by immunohisto-chemical assays. In addition, high FoxC1 expression was positively associated with lymph node metastasis, distant metastasis and an advanced clinical stage in patients with NPC. Furthermore, FoxC1 expression was high in NPC cell lines while being low in an immortalized normal nasopharyngeal epithelial cell line. In vitro, knockdown of FoxC1 in the CNE2 human NPC cell line by small interfering RNA downregulated vimentin, fibronectin and N-cadherin expression and reduced the migratory and invasive capacity of CNE2 cells. In conclusion, the present study indicated that FoxC1 has a pivotal role in EMT through the upregulation of vimentin, fibronectin and N-cadherin expression. Thus, FoxC1 may be a potential therapeutic target in NPC. D.A. Spandidos 2015-12 2015-10-12 /pmc/articles/PMC4758279/ /pubmed/26461269 http://dx.doi.org/10.3892/mmr.2015.4427 Text en Copyright: © Ou-Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
OU-YANG, LEI
XIAO, SHENG-JUN
LIU, PENG
YI, SHI-JANG
ZHANG, XIAO-LING
OU-YANG, SHI
TAN, SHENG-KUI
LEI, XUN
Forkhead box C1 induces epithelial-mesenchymal transition and is a potential therapeutic target in nasopharyngeal carcinoma
title Forkhead box C1 induces epithelial-mesenchymal transition and is a potential therapeutic target in nasopharyngeal carcinoma
title_full Forkhead box C1 induces epithelial-mesenchymal transition and is a potential therapeutic target in nasopharyngeal carcinoma
title_fullStr Forkhead box C1 induces epithelial-mesenchymal transition and is a potential therapeutic target in nasopharyngeal carcinoma
title_full_unstemmed Forkhead box C1 induces epithelial-mesenchymal transition and is a potential therapeutic target in nasopharyngeal carcinoma
title_short Forkhead box C1 induces epithelial-mesenchymal transition and is a potential therapeutic target in nasopharyngeal carcinoma
title_sort forkhead box c1 induces epithelial-mesenchymal transition and is a potential therapeutic target in nasopharyngeal carcinoma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4758279/
https://www.ncbi.nlm.nih.gov/pubmed/26461269
http://dx.doi.org/10.3892/mmr.2015.4427
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