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Liver mitochondrial function in ZDF rats during the early stages of diabetes disease

The aim of this study was to characterize the early alterations of the liver mitochondrial function in ZDF (fa/fa) rats that develop diabetes compared to that of their lean counterparts ZDF (fa/+). Liver mitochondrial function was examined in 11‐ and 14‐week‐old ZDF (fa/fa) and ZDF lean (fa/+) rats....

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Autores principales: Vial, Guillaume, Le Guen, Marie, Lamarche, Frédéric, Detaille, Dominique, Cottet‐Rousselle, Cécile, Demaison, Luc, Hininger‐Favier, Isabelle, Theurey, Pierre, Crouzier, David, Debouzy, Jean‐Claude, Dubouchaud, Hervé, Fontaine, Éric
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4758924/
https://www.ncbi.nlm.nih.gov/pubmed/26847727
http://dx.doi.org/10.14814/phy2.12686
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author Vial, Guillaume
Le Guen, Marie
Lamarche, Frédéric
Detaille, Dominique
Cottet‐Rousselle, Cécile
Demaison, Luc
Hininger‐Favier, Isabelle
Theurey, Pierre
Crouzier, David
Debouzy, Jean‐Claude
Dubouchaud, Hervé
Fontaine, Éric
author_facet Vial, Guillaume
Le Guen, Marie
Lamarche, Frédéric
Detaille, Dominique
Cottet‐Rousselle, Cécile
Demaison, Luc
Hininger‐Favier, Isabelle
Theurey, Pierre
Crouzier, David
Debouzy, Jean‐Claude
Dubouchaud, Hervé
Fontaine, Éric
author_sort Vial, Guillaume
collection PubMed
description The aim of this study was to characterize the early alterations of the liver mitochondrial function in ZDF (fa/fa) rats that develop diabetes compared to that of their lean counterparts ZDF (fa/+). Liver mitochondrial function was examined in 11‐ and 14‐week‐old ZDF (fa/fa) and ZDF lean (fa/+) rats. Oxygen consumption, H(2)O(2) release, calcium retention capacity (CRC), membrane potential, membrane fluidity, and fatty acid composition were analyzed. State 3 oxygen consumption with palmitoyl‐carnitine increases between 11 and 14 weeks of age in lean but not in diabetic animals. This response was not seen with other substrates, suggesting that the use of fatty acids is impaired in diabetic rats. H(2)O(2) release was lower in 14‐week‐old ZDF (fa/fa) rats as compared to ZDF lean (fa/+). These changes were not associated with differences in enzymatic activities of the respiratory complexes, suggesting regulatory mechanisms independent of their expression levels. Membrane fluidity and composition analyses show only slight effects linked to diabetes progression. The most salient feature was a reduction in CRC in the presence of CsA, an effect reflecting PTP dysregulation. Our data suggest few changes of mitochondrial function in ZDF fa/fa rats. At the age of 11 weeks, liver mitochondria have mainly a reduced effect of CsA on CRC.
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spelling pubmed-47589242016-02-29 Liver mitochondrial function in ZDF rats during the early stages of diabetes disease Vial, Guillaume Le Guen, Marie Lamarche, Frédéric Detaille, Dominique Cottet‐Rousselle, Cécile Demaison, Luc Hininger‐Favier, Isabelle Theurey, Pierre Crouzier, David Debouzy, Jean‐Claude Dubouchaud, Hervé Fontaine, Éric Physiol Rep Original Research The aim of this study was to characterize the early alterations of the liver mitochondrial function in ZDF (fa/fa) rats that develop diabetes compared to that of their lean counterparts ZDF (fa/+). Liver mitochondrial function was examined in 11‐ and 14‐week‐old ZDF (fa/fa) and ZDF lean (fa/+) rats. Oxygen consumption, H(2)O(2) release, calcium retention capacity (CRC), membrane potential, membrane fluidity, and fatty acid composition were analyzed. State 3 oxygen consumption with palmitoyl‐carnitine increases between 11 and 14 weeks of age in lean but not in diabetic animals. This response was not seen with other substrates, suggesting that the use of fatty acids is impaired in diabetic rats. H(2)O(2) release was lower in 14‐week‐old ZDF (fa/fa) rats as compared to ZDF lean (fa/+). These changes were not associated with differences in enzymatic activities of the respiratory complexes, suggesting regulatory mechanisms independent of their expression levels. Membrane fluidity and composition analyses show only slight effects linked to diabetes progression. The most salient feature was a reduction in CRC in the presence of CsA, an effect reflecting PTP dysregulation. Our data suggest few changes of mitochondrial function in ZDF fa/fa rats. At the age of 11 weeks, liver mitochondria have mainly a reduced effect of CsA on CRC. John Wiley and Sons Inc. 2016-02-04 /pmc/articles/PMC4758924/ /pubmed/26847727 http://dx.doi.org/10.14814/phy2.12686 Text en © 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Vial, Guillaume
Le Guen, Marie
Lamarche, Frédéric
Detaille, Dominique
Cottet‐Rousselle, Cécile
Demaison, Luc
Hininger‐Favier, Isabelle
Theurey, Pierre
Crouzier, David
Debouzy, Jean‐Claude
Dubouchaud, Hervé
Fontaine, Éric
Liver mitochondrial function in ZDF rats during the early stages of diabetes disease
title Liver mitochondrial function in ZDF rats during the early stages of diabetes disease
title_full Liver mitochondrial function in ZDF rats during the early stages of diabetes disease
title_fullStr Liver mitochondrial function in ZDF rats during the early stages of diabetes disease
title_full_unstemmed Liver mitochondrial function in ZDF rats during the early stages of diabetes disease
title_short Liver mitochondrial function in ZDF rats during the early stages of diabetes disease
title_sort liver mitochondrial function in zdf rats during the early stages of diabetes disease
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4758924/
https://www.ncbi.nlm.nih.gov/pubmed/26847727
http://dx.doi.org/10.14814/phy2.12686
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