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Liver mitochondrial function in ZDF rats during the early stages of diabetes disease
The aim of this study was to characterize the early alterations of the liver mitochondrial function in ZDF (fa/fa) rats that develop diabetes compared to that of their lean counterparts ZDF (fa/+). Liver mitochondrial function was examined in 11‐ and 14‐week‐old ZDF (fa/fa) and ZDF lean (fa/+) rats....
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4758924/ https://www.ncbi.nlm.nih.gov/pubmed/26847727 http://dx.doi.org/10.14814/phy2.12686 |
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author | Vial, Guillaume Le Guen, Marie Lamarche, Frédéric Detaille, Dominique Cottet‐Rousselle, Cécile Demaison, Luc Hininger‐Favier, Isabelle Theurey, Pierre Crouzier, David Debouzy, Jean‐Claude Dubouchaud, Hervé Fontaine, Éric |
author_facet | Vial, Guillaume Le Guen, Marie Lamarche, Frédéric Detaille, Dominique Cottet‐Rousselle, Cécile Demaison, Luc Hininger‐Favier, Isabelle Theurey, Pierre Crouzier, David Debouzy, Jean‐Claude Dubouchaud, Hervé Fontaine, Éric |
author_sort | Vial, Guillaume |
collection | PubMed |
description | The aim of this study was to characterize the early alterations of the liver mitochondrial function in ZDF (fa/fa) rats that develop diabetes compared to that of their lean counterparts ZDF (fa/+). Liver mitochondrial function was examined in 11‐ and 14‐week‐old ZDF (fa/fa) and ZDF lean (fa/+) rats. Oxygen consumption, H(2)O(2) release, calcium retention capacity (CRC), membrane potential, membrane fluidity, and fatty acid composition were analyzed. State 3 oxygen consumption with palmitoyl‐carnitine increases between 11 and 14 weeks of age in lean but not in diabetic animals. This response was not seen with other substrates, suggesting that the use of fatty acids is impaired in diabetic rats. H(2)O(2) release was lower in 14‐week‐old ZDF (fa/fa) rats as compared to ZDF lean (fa/+). These changes were not associated with differences in enzymatic activities of the respiratory complexes, suggesting regulatory mechanisms independent of their expression levels. Membrane fluidity and composition analyses show only slight effects linked to diabetes progression. The most salient feature was a reduction in CRC in the presence of CsA, an effect reflecting PTP dysregulation. Our data suggest few changes of mitochondrial function in ZDF fa/fa rats. At the age of 11 weeks, liver mitochondria have mainly a reduced effect of CsA on CRC. |
format | Online Article Text |
id | pubmed-4758924 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47589242016-02-29 Liver mitochondrial function in ZDF rats during the early stages of diabetes disease Vial, Guillaume Le Guen, Marie Lamarche, Frédéric Detaille, Dominique Cottet‐Rousselle, Cécile Demaison, Luc Hininger‐Favier, Isabelle Theurey, Pierre Crouzier, David Debouzy, Jean‐Claude Dubouchaud, Hervé Fontaine, Éric Physiol Rep Original Research The aim of this study was to characterize the early alterations of the liver mitochondrial function in ZDF (fa/fa) rats that develop diabetes compared to that of their lean counterparts ZDF (fa/+). Liver mitochondrial function was examined in 11‐ and 14‐week‐old ZDF (fa/fa) and ZDF lean (fa/+) rats. Oxygen consumption, H(2)O(2) release, calcium retention capacity (CRC), membrane potential, membrane fluidity, and fatty acid composition were analyzed. State 3 oxygen consumption with palmitoyl‐carnitine increases between 11 and 14 weeks of age in lean but not in diabetic animals. This response was not seen with other substrates, suggesting that the use of fatty acids is impaired in diabetic rats. H(2)O(2) release was lower in 14‐week‐old ZDF (fa/fa) rats as compared to ZDF lean (fa/+). These changes were not associated with differences in enzymatic activities of the respiratory complexes, suggesting regulatory mechanisms independent of their expression levels. Membrane fluidity and composition analyses show only slight effects linked to diabetes progression. The most salient feature was a reduction in CRC in the presence of CsA, an effect reflecting PTP dysregulation. Our data suggest few changes of mitochondrial function in ZDF fa/fa rats. At the age of 11 weeks, liver mitochondria have mainly a reduced effect of CsA on CRC. John Wiley and Sons Inc. 2016-02-04 /pmc/articles/PMC4758924/ /pubmed/26847727 http://dx.doi.org/10.14814/phy2.12686 Text en © 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Vial, Guillaume Le Guen, Marie Lamarche, Frédéric Detaille, Dominique Cottet‐Rousselle, Cécile Demaison, Luc Hininger‐Favier, Isabelle Theurey, Pierre Crouzier, David Debouzy, Jean‐Claude Dubouchaud, Hervé Fontaine, Éric Liver mitochondrial function in ZDF rats during the early stages of diabetes disease |
title | Liver mitochondrial function in ZDF rats during the early stages of diabetes disease |
title_full | Liver mitochondrial function in ZDF rats during the early stages of diabetes disease |
title_fullStr | Liver mitochondrial function in ZDF rats during the early stages of diabetes disease |
title_full_unstemmed | Liver mitochondrial function in ZDF rats during the early stages of diabetes disease |
title_short | Liver mitochondrial function in ZDF rats during the early stages of diabetes disease |
title_sort | liver mitochondrial function in zdf rats during the early stages of diabetes disease |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4758924/ https://www.ncbi.nlm.nih.gov/pubmed/26847727 http://dx.doi.org/10.14814/phy2.12686 |
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