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Detection of canine vector-borne diseases in eastern Poland by ELISA and PCR
The aim of the study was to establish the prevalence of Ehrlichia canis, Anaplasma phagocytophilum and Borrelia burgdorferi in dogs in eastern Poland and to determine the factors associated with exposure (seroposity) or infection (PCR). Anti-A. phagocytophilum, anti-B. burgdorferi and anti-E. canis...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759218/ https://www.ncbi.nlm.nih.gov/pubmed/26581374 http://dx.doi.org/10.1007/s00436-015-4832-1 |
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author | Dzięgiel, Beata Adaszek, Łukasz Carbonero, Alfonso Łyp, Paweł Winiarczyk, Mateusz Dębiak, Piotr Winiarczyk, Stanisław |
author_facet | Dzięgiel, Beata Adaszek, Łukasz Carbonero, Alfonso Łyp, Paweł Winiarczyk, Mateusz Dębiak, Piotr Winiarczyk, Stanisław |
author_sort | Dzięgiel, Beata |
collection | PubMed |
description | The aim of the study was to establish the prevalence of Ehrlichia canis, Anaplasma phagocytophilum and Borrelia burgdorferi in dogs in eastern Poland and to determine the factors associated with exposure (seroposity) or infection (PCR). Anti-A. phagocytophilum, anti-B. burgdorferi and anti-E. canis antibodies were determined in 400 dogs, using the SNAP 4Dx ® test (IDEXX Laboratories). In addition, PCRs were performed for the detection of E. canis, A. phagocytophilum and B. burgdorferi DNA. In reference to the risk factor analysis, a regression logistic model was determined for each aetiological agent. The overall seroprevalence was highest for B. burgdorferi (11.0 %), followed by A. phagocytophilum (8.0 %) and E. canis (1.5 %). Eleven healthy dogs were found to be infected with A. phagocytophilum, as determined by PCR, while the remainder were seronegative. For B. burgdorferi, the DNA of the spirochetes was detected in the blood of 20 dogs, while the presence of anti-B. burgdorferi IgG was detected in the sera of ten of these. For E. canis, none of the dogs tested positive by PCR. Tick control was included as a protective factor for A. phagocytophilum and B. burgdorferi, while the origin (rural) was included as a risk factor for B. burgdorferi and A. phagocytophilum infection. In addition, breed (pure) was a risk factor for B. burgdorferi infection, and sex (female) was a risk factor for E. canis. |
format | Online Article Text |
id | pubmed-4759218 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-47592182016-02-29 Detection of canine vector-borne diseases in eastern Poland by ELISA and PCR Dzięgiel, Beata Adaszek, Łukasz Carbonero, Alfonso Łyp, Paweł Winiarczyk, Mateusz Dębiak, Piotr Winiarczyk, Stanisław Parasitol Res Original Paper The aim of the study was to establish the prevalence of Ehrlichia canis, Anaplasma phagocytophilum and Borrelia burgdorferi in dogs in eastern Poland and to determine the factors associated with exposure (seroposity) or infection (PCR). Anti-A. phagocytophilum, anti-B. burgdorferi and anti-E. canis antibodies were determined in 400 dogs, using the SNAP 4Dx ® test (IDEXX Laboratories). In addition, PCRs were performed for the detection of E. canis, A. phagocytophilum and B. burgdorferi DNA. In reference to the risk factor analysis, a regression logistic model was determined for each aetiological agent. The overall seroprevalence was highest for B. burgdorferi (11.0 %), followed by A. phagocytophilum (8.0 %) and E. canis (1.5 %). Eleven healthy dogs were found to be infected with A. phagocytophilum, as determined by PCR, while the remainder were seronegative. For B. burgdorferi, the DNA of the spirochetes was detected in the blood of 20 dogs, while the presence of anti-B. burgdorferi IgG was detected in the sera of ten of these. For E. canis, none of the dogs tested positive by PCR. Tick control was included as a protective factor for A. phagocytophilum and B. burgdorferi, while the origin (rural) was included as a risk factor for B. burgdorferi and A. phagocytophilum infection. In addition, breed (pure) was a risk factor for B. burgdorferi infection, and sex (female) was a risk factor for E. canis. Springer Berlin Heidelberg 2015-11-19 2016 /pmc/articles/PMC4759218/ /pubmed/26581374 http://dx.doi.org/10.1007/s00436-015-4832-1 Text en © The Author(s) 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Paper Dzięgiel, Beata Adaszek, Łukasz Carbonero, Alfonso Łyp, Paweł Winiarczyk, Mateusz Dębiak, Piotr Winiarczyk, Stanisław Detection of canine vector-borne diseases in eastern Poland by ELISA and PCR |
title | Detection of canine vector-borne diseases in eastern Poland by ELISA and PCR |
title_full | Detection of canine vector-borne diseases in eastern Poland by ELISA and PCR |
title_fullStr | Detection of canine vector-borne diseases in eastern Poland by ELISA and PCR |
title_full_unstemmed | Detection of canine vector-borne diseases in eastern Poland by ELISA and PCR |
title_short | Detection of canine vector-borne diseases in eastern Poland by ELISA and PCR |
title_sort | detection of canine vector-borne diseases in eastern poland by elisa and pcr |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759218/ https://www.ncbi.nlm.nih.gov/pubmed/26581374 http://dx.doi.org/10.1007/s00436-015-4832-1 |
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