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Kingdom-Wide Analysis of Fungal Small Secreted Proteins (SSPs) Reveals their Potential Role in Host Association

Fungal secretome consists of various functional groups of proteins, many of which participate in nutrient acquisition, self-protection, or manipulation of the environment and neighboring organisms. The least characterized component of the secretome is small secreted proteins (SSPs). Some SSPs have b...

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Autores principales: Kim, Ki-Tae, Jeon, Jongbum, Choi, Jaeyoung, Cheong, Kyeongchae, Song, Hyeunjeong, Choi, Gobong, Kang, Seogchan, Lee, Yong-Hwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759460/
https://www.ncbi.nlm.nih.gov/pubmed/26925088
http://dx.doi.org/10.3389/fpls.2016.00186
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author Kim, Ki-Tae
Jeon, Jongbum
Choi, Jaeyoung
Cheong, Kyeongchae
Song, Hyeunjeong
Choi, Gobong
Kang, Seogchan
Lee, Yong-Hwan
author_facet Kim, Ki-Tae
Jeon, Jongbum
Choi, Jaeyoung
Cheong, Kyeongchae
Song, Hyeunjeong
Choi, Gobong
Kang, Seogchan
Lee, Yong-Hwan
author_sort Kim, Ki-Tae
collection PubMed
description Fungal secretome consists of various functional groups of proteins, many of which participate in nutrient acquisition, self-protection, or manipulation of the environment and neighboring organisms. The least characterized component of the secretome is small secreted proteins (SSPs). Some SSPs have been reported to function as effectors, but most remain to be characterized. The composition of major secretome components, such as carbohydrate-active enzymes, proteases, lipases, and oxidoreductases, appear to reflect the lifestyle and ecological niche of individual species. We hypothesize that many SSPs participate in manipulating plants as effectors. Obligate biotrophs likely encode more and diverse effector-like SSPs to suppress host defense compared to necrotrophs, which generally use cell wall degrading enzymes and phytotoxins to kill hosts. Because different secretome prediction workflows have been used in different studies, available secretome data are difficult to integrate for comprehensive comparative studies to test this hypothesis. In this study, SSPs encoded by 136 fungal species were identified from data archived in Fungal Secretome Database (FSD) via a refined secretome workflow. Subsequently, compositions of SSPs and other secretome components were compared in light of taxa and lifestyles. Those species that are intimately associated with host cells, such as biotrophs and symbionts, usually have higher proportion of species-specific SSPs (SSSPs) than hemibiotrophs and necrotrophs, but the latter groups displayed higher proportions of secreted enzymes. Results from our study established a foundation for functional studies on SSPs and will also help understand genomic changes potentially underpinning different fungal lifestyles.
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spelling pubmed-47594602016-02-26 Kingdom-Wide Analysis of Fungal Small Secreted Proteins (SSPs) Reveals their Potential Role in Host Association Kim, Ki-Tae Jeon, Jongbum Choi, Jaeyoung Cheong, Kyeongchae Song, Hyeunjeong Choi, Gobong Kang, Seogchan Lee, Yong-Hwan Front Plant Sci Plant Science Fungal secretome consists of various functional groups of proteins, many of which participate in nutrient acquisition, self-protection, or manipulation of the environment and neighboring organisms. The least characterized component of the secretome is small secreted proteins (SSPs). Some SSPs have been reported to function as effectors, but most remain to be characterized. The composition of major secretome components, such as carbohydrate-active enzymes, proteases, lipases, and oxidoreductases, appear to reflect the lifestyle and ecological niche of individual species. We hypothesize that many SSPs participate in manipulating plants as effectors. Obligate biotrophs likely encode more and diverse effector-like SSPs to suppress host defense compared to necrotrophs, which generally use cell wall degrading enzymes and phytotoxins to kill hosts. Because different secretome prediction workflows have been used in different studies, available secretome data are difficult to integrate for comprehensive comparative studies to test this hypothesis. In this study, SSPs encoded by 136 fungal species were identified from data archived in Fungal Secretome Database (FSD) via a refined secretome workflow. Subsequently, compositions of SSPs and other secretome components were compared in light of taxa and lifestyles. Those species that are intimately associated with host cells, such as biotrophs and symbionts, usually have higher proportion of species-specific SSPs (SSSPs) than hemibiotrophs and necrotrophs, but the latter groups displayed higher proportions of secreted enzymes. Results from our study established a foundation for functional studies on SSPs and will also help understand genomic changes potentially underpinning different fungal lifestyles. Frontiers Media S.A. 2016-02-19 /pmc/articles/PMC4759460/ /pubmed/26925088 http://dx.doi.org/10.3389/fpls.2016.00186 Text en Copyright © 2016 Kim, Jeon, Choi, Cheong, Song, Choi, Kang and Lee. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Kim, Ki-Tae
Jeon, Jongbum
Choi, Jaeyoung
Cheong, Kyeongchae
Song, Hyeunjeong
Choi, Gobong
Kang, Seogchan
Lee, Yong-Hwan
Kingdom-Wide Analysis of Fungal Small Secreted Proteins (SSPs) Reveals their Potential Role in Host Association
title Kingdom-Wide Analysis of Fungal Small Secreted Proteins (SSPs) Reveals their Potential Role in Host Association
title_full Kingdom-Wide Analysis of Fungal Small Secreted Proteins (SSPs) Reveals their Potential Role in Host Association
title_fullStr Kingdom-Wide Analysis of Fungal Small Secreted Proteins (SSPs) Reveals their Potential Role in Host Association
title_full_unstemmed Kingdom-Wide Analysis of Fungal Small Secreted Proteins (SSPs) Reveals their Potential Role in Host Association
title_short Kingdom-Wide Analysis of Fungal Small Secreted Proteins (SSPs) Reveals their Potential Role in Host Association
title_sort kingdom-wide analysis of fungal small secreted proteins (ssps) reveals their potential role in host association
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759460/
https://www.ncbi.nlm.nih.gov/pubmed/26925088
http://dx.doi.org/10.3389/fpls.2016.00186
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