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cGMP Signalling Mediates Water Sensation (Hydrosensation) and Hydrotaxis in Caenorhabditis elegans
Animals have developed the ability to sense the water content in their habitats, including hygrosensation (sensing humidity in the air) and hydrosensation (sensing the water content in other microenvironments), and they display preferences for specific water contents that influence their mating, rep...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759535/ https://www.ncbi.nlm.nih.gov/pubmed/26891989 http://dx.doi.org/10.1038/srep19779 |
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author | Wang, Wei Qin, Li-Wei Wu, Tai-Hong Ge, Chang-Li Wu, Ya-Qian Zhang, Qiang Song, Yan-Xue Chen, Yuan-Hua Ge, Ming-Hai Wu, Jing-Jing Liu, Hui Xu, Yao Su, Chun-Ming Li, Lan-Lan Tang, Jing Li, Zhao-Yu Wu, Zheng-Xing |
author_facet | Wang, Wei Qin, Li-Wei Wu, Tai-Hong Ge, Chang-Li Wu, Ya-Qian Zhang, Qiang Song, Yan-Xue Chen, Yuan-Hua Ge, Ming-Hai Wu, Jing-Jing Liu, Hui Xu, Yao Su, Chun-Ming Li, Lan-Lan Tang, Jing Li, Zhao-Yu Wu, Zheng-Xing |
author_sort | Wang, Wei |
collection | PubMed |
description | Animals have developed the ability to sense the water content in their habitats, including hygrosensation (sensing humidity in the air) and hydrosensation (sensing the water content in other microenvironments), and they display preferences for specific water contents that influence their mating, reproduction and geographic distribution. We developed and employed four quantitative behavioural test paradigms to investigate the molecular and cellular mechanisms underlying sensing the water content in an agar substrate (hydrosensation) and hydrotaxis in Caenorhabditis elegans. By combining a reverse genetic screen with genetic manipulation, optogenetic neuronal manipulation and in vivo Ca(2+) imaging, we demonstrate that adult worms avoid the wetter areas of agar plates and hypo-osmotic water droplets. We found that the cGMP signalling pathway in ciliated sensory neurons is involved in hydrosensation and hydrotaxis in Caenorhabditis elegans. |
format | Online Article Text |
id | pubmed-4759535 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47595352016-02-26 cGMP Signalling Mediates Water Sensation (Hydrosensation) and Hydrotaxis in Caenorhabditis elegans Wang, Wei Qin, Li-Wei Wu, Tai-Hong Ge, Chang-Li Wu, Ya-Qian Zhang, Qiang Song, Yan-Xue Chen, Yuan-Hua Ge, Ming-Hai Wu, Jing-Jing Liu, Hui Xu, Yao Su, Chun-Ming Li, Lan-Lan Tang, Jing Li, Zhao-Yu Wu, Zheng-Xing Sci Rep Article Animals have developed the ability to sense the water content in their habitats, including hygrosensation (sensing humidity in the air) and hydrosensation (sensing the water content in other microenvironments), and they display preferences for specific water contents that influence their mating, reproduction and geographic distribution. We developed and employed four quantitative behavioural test paradigms to investigate the molecular and cellular mechanisms underlying sensing the water content in an agar substrate (hydrosensation) and hydrotaxis in Caenorhabditis elegans. By combining a reverse genetic screen with genetic manipulation, optogenetic neuronal manipulation and in vivo Ca(2+) imaging, we demonstrate that adult worms avoid the wetter areas of agar plates and hypo-osmotic water droplets. We found that the cGMP signalling pathway in ciliated sensory neurons is involved in hydrosensation and hydrotaxis in Caenorhabditis elegans. Nature Publishing Group 2016-02-19 /pmc/articles/PMC4759535/ /pubmed/26891989 http://dx.doi.org/10.1038/srep19779 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Wang, Wei Qin, Li-Wei Wu, Tai-Hong Ge, Chang-Li Wu, Ya-Qian Zhang, Qiang Song, Yan-Xue Chen, Yuan-Hua Ge, Ming-Hai Wu, Jing-Jing Liu, Hui Xu, Yao Su, Chun-Ming Li, Lan-Lan Tang, Jing Li, Zhao-Yu Wu, Zheng-Xing cGMP Signalling Mediates Water Sensation (Hydrosensation) and Hydrotaxis in Caenorhabditis elegans |
title | cGMP Signalling Mediates Water Sensation (Hydrosensation) and Hydrotaxis in Caenorhabditis elegans |
title_full | cGMP Signalling Mediates Water Sensation (Hydrosensation) and Hydrotaxis in Caenorhabditis elegans |
title_fullStr | cGMP Signalling Mediates Water Sensation (Hydrosensation) and Hydrotaxis in Caenorhabditis elegans |
title_full_unstemmed | cGMP Signalling Mediates Water Sensation (Hydrosensation) and Hydrotaxis in Caenorhabditis elegans |
title_short | cGMP Signalling Mediates Water Sensation (Hydrosensation) and Hydrotaxis in Caenorhabditis elegans |
title_sort | cgmp signalling mediates water sensation (hydrosensation) and hydrotaxis in caenorhabditis elegans |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759535/ https://www.ncbi.nlm.nih.gov/pubmed/26891989 http://dx.doi.org/10.1038/srep19779 |
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