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Smoking Induced Hemolysis: Spectral and microscopic investigations

Smoking is one of the major causes of lifestyle associated mortality and morbidity such as cancer of the oral cavity and lungs, and also cardiovascular diseases. In this study, we have provided evidences for the smoking-induced hemolysis using two methods: spectra of blood components and atomic forc...

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Autores principales: Masilamani, Vadivel, AlZahrani, Khalid, Devanesan, Sandhanasamy, AlQahtani, Hadi, AlSalhi, Mohamad Saleh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759537/
https://www.ncbi.nlm.nih.gov/pubmed/26891995
http://dx.doi.org/10.1038/srep21095
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author Masilamani, Vadivel
AlZahrani, Khalid
Devanesan, Sandhanasamy
AlQahtani, Hadi
AlSalhi, Mohamad Saleh
author_facet Masilamani, Vadivel
AlZahrani, Khalid
Devanesan, Sandhanasamy
AlQahtani, Hadi
AlSalhi, Mohamad Saleh
author_sort Masilamani, Vadivel
collection PubMed
description Smoking is one of the major causes of lifestyle associated mortality and morbidity such as cancer of the oral cavity and lungs, and also cardiovascular diseases. In this study, we have provided evidences for the smoking-induced hemolysis using two methods: spectra of blood components and atomic force microscopic analysis of surface morphology. A total of 62 subjects (control = 31; smoker = 31: 21 male; 10 female in each set) were considered for the study. The findings indicate that smoking leads to potholes on the surface, swelling of shape, rupturing of erythrocytes, removal of hematoporphyrin and flushing into the plasma as metabolites of the erythrocyte. The overall morphology of the erythrocytes of the smoker group appears more like a Mexican hat. The mean surface roughness was 5.5 ± 3 nm for the smoker group, but 1.2 ± 0.2 nm for the control group. Such damages might help the toxins, (CO, peroxidants, aldehydes etc.,) to gain easy access and get strongly absorbed by the hemoglobin, leading to enhanced rates of hemolysis as shown by the spectral features of metabolites. This indicates that the average life span of the smoker’s erythrocytes is significantly less than that of the control group.
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spelling pubmed-47595372016-02-26 Smoking Induced Hemolysis: Spectral and microscopic investigations Masilamani, Vadivel AlZahrani, Khalid Devanesan, Sandhanasamy AlQahtani, Hadi AlSalhi, Mohamad Saleh Sci Rep Article Smoking is one of the major causes of lifestyle associated mortality and morbidity such as cancer of the oral cavity and lungs, and also cardiovascular diseases. In this study, we have provided evidences for the smoking-induced hemolysis using two methods: spectra of blood components and atomic force microscopic analysis of surface morphology. A total of 62 subjects (control = 31; smoker = 31: 21 male; 10 female in each set) were considered for the study. The findings indicate that smoking leads to potholes on the surface, swelling of shape, rupturing of erythrocytes, removal of hematoporphyrin and flushing into the plasma as metabolites of the erythrocyte. The overall morphology of the erythrocytes of the smoker group appears more like a Mexican hat. The mean surface roughness was 5.5 ± 3 nm for the smoker group, but 1.2 ± 0.2 nm for the control group. Such damages might help the toxins, (CO, peroxidants, aldehydes etc.,) to gain easy access and get strongly absorbed by the hemoglobin, leading to enhanced rates of hemolysis as shown by the spectral features of metabolites. This indicates that the average life span of the smoker’s erythrocytes is significantly less than that of the control group. Nature Publishing Group 2016-02-19 /pmc/articles/PMC4759537/ /pubmed/26891995 http://dx.doi.org/10.1038/srep21095 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Masilamani, Vadivel
AlZahrani, Khalid
Devanesan, Sandhanasamy
AlQahtani, Hadi
AlSalhi, Mohamad Saleh
Smoking Induced Hemolysis: Spectral and microscopic investigations
title Smoking Induced Hemolysis: Spectral and microscopic investigations
title_full Smoking Induced Hemolysis: Spectral and microscopic investigations
title_fullStr Smoking Induced Hemolysis: Spectral and microscopic investigations
title_full_unstemmed Smoking Induced Hemolysis: Spectral and microscopic investigations
title_short Smoking Induced Hemolysis: Spectral and microscopic investigations
title_sort smoking induced hemolysis: spectral and microscopic investigations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759537/
https://www.ncbi.nlm.nih.gov/pubmed/26891995
http://dx.doi.org/10.1038/srep21095
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