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Purity matters: A workflow for the valid high-resolution lipid profiling of mitochondria from cell culture samples
Subcellular lipidomics is a novel field of research that requires the careful combination of several pre-analytical and analytical steps. To define a reliable strategy for mitochondrial lipid profiling, we performed a systematic comparison of different mitochondria isolation procedures by western bl...
Autores principales: | Kappler, Lisa, Li, Jia, Häring, Hans-Ulrich, Weigert, Cora, Lehmann, Rainer, Xu, Guowang, Hoene, Miriam |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759577/ https://www.ncbi.nlm.nih.gov/pubmed/26892142 http://dx.doi.org/10.1038/srep21107 |
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