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Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells

Transmembrane protein 14A (TMEM14A) is a member of TMEMs. Alterations in TMEMs expression have been identified in several types of cancer, but the expression and function of TMEM14A in ovarian cancer is still unclear. Here, analysis on the expression data of the Cancer Genome Atlas (TCGA) ovarian se...

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Autores principales: Zhang, Qingmei, Chen, Xiufeng, Zhang, Xuan, Zhan, Jingfen, Chen, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759611/
https://www.ncbi.nlm.nih.gov/pubmed/26896463
http://dx.doi.org/10.1042/BSR20150258
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author Zhang, Qingmei
Chen, Xiufeng
Zhang, Xuan
Zhan, Jingfen
Chen, Jie
author_facet Zhang, Qingmei
Chen, Xiufeng
Zhang, Xuan
Zhan, Jingfen
Chen, Jie
author_sort Zhang, Qingmei
collection PubMed
description Transmembrane protein 14A (TMEM14A) is a member of TMEMs. Alterations in TMEMs expression have been identified in several types of cancer, but the expression and function of TMEM14A in ovarian cancer is still unclear. Here, analysis on the expression data of the Cancer Genome Atlas (TCGA) ovarian serous cystadenocarcinoma (OV) dataset demonstrated the overexpression of TMEM14A in ovarian cancer tissues compared with normal tissues, which was consistent with our real-time PCR analysis on ovarian cancer and normal tissues collected from 30 patients. In addition, TMEM14A knockdown in two ovarian cancer cell lines, A2780 and HO-8910, reduced cell proliferation, causes cell cycle arrest and suppressed cell invasion. Moreover, silencing of TMEM14A notably repressed G1/S cell cycle transition and cell invasion via down-regulating the expression of cell cycle related proteins (Cyclin D1, Cyclin E and PCNA) and metastasis-related proteins (MMP-2 and MMP-9), respectively. TMEM14A knockdown significantly reduced the phosphorylation status of Smad2 and Smad3, downstream effectors of TGF-β signalling. In summary, these results indicate that TMEM14A has a pro-tumorigenic effect in ovarian cancer cells, suggesting an important role of this protein in ovarian cancer oncogenesis and metastasis.
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spelling pubmed-47596112016-03-03 Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells Zhang, Qingmei Chen, Xiufeng Zhang, Xuan Zhan, Jingfen Chen, Jie Biosci Rep Original Papers Transmembrane protein 14A (TMEM14A) is a member of TMEMs. Alterations in TMEMs expression have been identified in several types of cancer, but the expression and function of TMEM14A in ovarian cancer is still unclear. Here, analysis on the expression data of the Cancer Genome Atlas (TCGA) ovarian serous cystadenocarcinoma (OV) dataset demonstrated the overexpression of TMEM14A in ovarian cancer tissues compared with normal tissues, which was consistent with our real-time PCR analysis on ovarian cancer and normal tissues collected from 30 patients. In addition, TMEM14A knockdown in two ovarian cancer cell lines, A2780 and HO-8910, reduced cell proliferation, causes cell cycle arrest and suppressed cell invasion. Moreover, silencing of TMEM14A notably repressed G1/S cell cycle transition and cell invasion via down-regulating the expression of cell cycle related proteins (Cyclin D1, Cyclin E and PCNA) and metastasis-related proteins (MMP-2 and MMP-9), respectively. TMEM14A knockdown significantly reduced the phosphorylation status of Smad2 and Smad3, downstream effectors of TGF-β signalling. In summary, these results indicate that TMEM14A has a pro-tumorigenic effect in ovarian cancer cells, suggesting an important role of this protein in ovarian cancer oncogenesis and metastasis. Portland Press Ltd. 2016-02-19 /pmc/articles/PMC4759611/ /pubmed/26896463 http://dx.doi.org/10.1042/BSR20150258 Text en © 2016 Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article published by Portland Press Limited and distributed under the Creative Commons Attribution Licence 3.0 (http://creativecommons.org/licenses/by/3.0/) .
spellingShingle Original Papers
Zhang, Qingmei
Chen, Xiufeng
Zhang, Xuan
Zhan, Jingfen
Chen, Jie
Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells
title Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells
title_full Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells
title_fullStr Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells
title_full_unstemmed Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells
title_short Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells
title_sort knockdown of tmem14a expression by rnai inhibits the proliferation and invasion of human ovarian cancer cells
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759611/
https://www.ncbi.nlm.nih.gov/pubmed/26896463
http://dx.doi.org/10.1042/BSR20150258
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