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Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells
Transmembrane protein 14A (TMEM14A) is a member of TMEMs. Alterations in TMEMs expression have been identified in several types of cancer, but the expression and function of TMEM14A in ovarian cancer is still unclear. Here, analysis on the expression data of the Cancer Genome Atlas (TCGA) ovarian se...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759611/ https://www.ncbi.nlm.nih.gov/pubmed/26896463 http://dx.doi.org/10.1042/BSR20150258 |
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author | Zhang, Qingmei Chen, Xiufeng Zhang, Xuan Zhan, Jingfen Chen, Jie |
author_facet | Zhang, Qingmei Chen, Xiufeng Zhang, Xuan Zhan, Jingfen Chen, Jie |
author_sort | Zhang, Qingmei |
collection | PubMed |
description | Transmembrane protein 14A (TMEM14A) is a member of TMEMs. Alterations in TMEMs expression have been identified in several types of cancer, but the expression and function of TMEM14A in ovarian cancer is still unclear. Here, analysis on the expression data of the Cancer Genome Atlas (TCGA) ovarian serous cystadenocarcinoma (OV) dataset demonstrated the overexpression of TMEM14A in ovarian cancer tissues compared with normal tissues, which was consistent with our real-time PCR analysis on ovarian cancer and normal tissues collected from 30 patients. In addition, TMEM14A knockdown in two ovarian cancer cell lines, A2780 and HO-8910, reduced cell proliferation, causes cell cycle arrest and suppressed cell invasion. Moreover, silencing of TMEM14A notably repressed G1/S cell cycle transition and cell invasion via down-regulating the expression of cell cycle related proteins (Cyclin D1, Cyclin E and PCNA) and metastasis-related proteins (MMP-2 and MMP-9), respectively. TMEM14A knockdown significantly reduced the phosphorylation status of Smad2 and Smad3, downstream effectors of TGF-β signalling. In summary, these results indicate that TMEM14A has a pro-tumorigenic effect in ovarian cancer cells, suggesting an important role of this protein in ovarian cancer oncogenesis and metastasis. |
format | Online Article Text |
id | pubmed-4759611 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47596112016-03-03 Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells Zhang, Qingmei Chen, Xiufeng Zhang, Xuan Zhan, Jingfen Chen, Jie Biosci Rep Original Papers Transmembrane protein 14A (TMEM14A) is a member of TMEMs. Alterations in TMEMs expression have been identified in several types of cancer, but the expression and function of TMEM14A in ovarian cancer is still unclear. Here, analysis on the expression data of the Cancer Genome Atlas (TCGA) ovarian serous cystadenocarcinoma (OV) dataset demonstrated the overexpression of TMEM14A in ovarian cancer tissues compared with normal tissues, which was consistent with our real-time PCR analysis on ovarian cancer and normal tissues collected from 30 patients. In addition, TMEM14A knockdown in two ovarian cancer cell lines, A2780 and HO-8910, reduced cell proliferation, causes cell cycle arrest and suppressed cell invasion. Moreover, silencing of TMEM14A notably repressed G1/S cell cycle transition and cell invasion via down-regulating the expression of cell cycle related proteins (Cyclin D1, Cyclin E and PCNA) and metastasis-related proteins (MMP-2 and MMP-9), respectively. TMEM14A knockdown significantly reduced the phosphorylation status of Smad2 and Smad3, downstream effectors of TGF-β signalling. In summary, these results indicate that TMEM14A has a pro-tumorigenic effect in ovarian cancer cells, suggesting an important role of this protein in ovarian cancer oncogenesis and metastasis. Portland Press Ltd. 2016-02-19 /pmc/articles/PMC4759611/ /pubmed/26896463 http://dx.doi.org/10.1042/BSR20150258 Text en © 2016 Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article published by Portland Press Limited and distributed under the Creative Commons Attribution Licence 3.0 (http://creativecommons.org/licenses/by/3.0/) . |
spellingShingle | Original Papers Zhang, Qingmei Chen, Xiufeng Zhang, Xuan Zhan, Jingfen Chen, Jie Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells |
title | Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells |
title_full | Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells |
title_fullStr | Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells |
title_full_unstemmed | Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells |
title_short | Knockdown of TMEM14A expression by RNAi inhibits the proliferation and invasion of human ovarian cancer cells |
title_sort | knockdown of tmem14a expression by rnai inhibits the proliferation and invasion of human ovarian cancer cells |
topic | Original Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759611/ https://www.ncbi.nlm.nih.gov/pubmed/26896463 http://dx.doi.org/10.1042/BSR20150258 |
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