(18)F-Based Pretargeted PET Imaging Based on Bioorthogonal Diels–Alder Click Chemistry

[Image: see text] A first-of-its-kind (18)F pretargeted PET imaging approach based on the bioorthogonal inverse electron demand Diels–Alder (IEDDA) reaction between tetrazine (Tz) and trans-cyclooctene (TCO) is presented. As proof-of-principle, a TCO-bearing immunoconjugate of the anti-CA19.9 antibo...

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Autores principales: Meyer, Jan-Philip, Houghton, Jacob L., Kozlowski, Paul, Abdel-Atti, Dalya, Reiner, Thomas, Pillarsetty, Naga Vara Kishore, Scholz, Wolfgang W., Zeglis, Brian M., Lewis, Jason S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2015
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759614/
https://www.ncbi.nlm.nih.gov/pubmed/26479967
http://dx.doi.org/10.1021/acs.bioconjchem.5b00504
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author Meyer, Jan-Philip
Houghton, Jacob L.
Kozlowski, Paul
Abdel-Atti, Dalya
Reiner, Thomas
Pillarsetty, Naga Vara Kishore
Scholz, Wolfgang W.
Zeglis, Brian M.
Lewis, Jason S.
author_facet Meyer, Jan-Philip
Houghton, Jacob L.
Kozlowski, Paul
Abdel-Atti, Dalya
Reiner, Thomas
Pillarsetty, Naga Vara Kishore
Scholz, Wolfgang W.
Zeglis, Brian M.
Lewis, Jason S.
author_sort Meyer, Jan-Philip
collection PubMed
description [Image: see text] A first-of-its-kind (18)F pretargeted PET imaging approach based on the bioorthogonal inverse electron demand Diels–Alder (IEDDA) reaction between tetrazine (Tz) and trans-cyclooctene (TCO) is presented. As proof-of-principle, a TCO-bearing immunoconjugate of the anti-CA19.9 antibody 5B1 and an Al[(18)F]NOTA-labeled tetrazine radioligand were harnessed for the visualization of CA19.9-expressing BxPC3 pancreatic cancer xenografts. Biodistribution and (18)F-PET imaging data clearly demonstrate that this methodology effectively delineates tumor mass with activity concentrations up to 6.4 %ID/g at 4 h after injection of the radioligand.
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spelling pubmed-47596142016-02-29 (18)F-Based Pretargeted PET Imaging Based on Bioorthogonal Diels–Alder Click Chemistry Meyer, Jan-Philip Houghton, Jacob L. Kozlowski, Paul Abdel-Atti, Dalya Reiner, Thomas Pillarsetty, Naga Vara Kishore Scholz, Wolfgang W. Zeglis, Brian M. Lewis, Jason S. Bioconjug Chem [Image: see text] A first-of-its-kind (18)F pretargeted PET imaging approach based on the bioorthogonal inverse electron demand Diels–Alder (IEDDA) reaction between tetrazine (Tz) and trans-cyclooctene (TCO) is presented. As proof-of-principle, a TCO-bearing immunoconjugate of the anti-CA19.9 antibody 5B1 and an Al[(18)F]NOTA-labeled tetrazine radioligand were harnessed for the visualization of CA19.9-expressing BxPC3 pancreatic cancer xenografts. Biodistribution and (18)F-PET imaging data clearly demonstrate that this methodology effectively delineates tumor mass with activity concentrations up to 6.4 %ID/g at 4 h after injection of the radioligand. American Chemical Society 2015-10-19 2016-02-17 /pmc/articles/PMC4759614/ /pubmed/26479967 http://dx.doi.org/10.1021/acs.bioconjchem.5b00504 Text en Copyright © 2015 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Meyer, Jan-Philip
Houghton, Jacob L.
Kozlowski, Paul
Abdel-Atti, Dalya
Reiner, Thomas
Pillarsetty, Naga Vara Kishore
Scholz, Wolfgang W.
Zeglis, Brian M.
Lewis, Jason S.
(18)F-Based Pretargeted PET Imaging Based on Bioorthogonal Diels–Alder Click Chemistry
title (18)F-Based Pretargeted PET Imaging Based on Bioorthogonal Diels–Alder Click Chemistry
title_full (18)F-Based Pretargeted PET Imaging Based on Bioorthogonal Diels–Alder Click Chemistry
title_fullStr (18)F-Based Pretargeted PET Imaging Based on Bioorthogonal Diels–Alder Click Chemistry
title_full_unstemmed (18)F-Based Pretargeted PET Imaging Based on Bioorthogonal Diels–Alder Click Chemistry
title_short (18)F-Based Pretargeted PET Imaging Based on Bioorthogonal Diels–Alder Click Chemistry
title_sort (18)f-based pretargeted pet imaging based on bioorthogonal diels–alder click chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759614/
https://www.ncbi.nlm.nih.gov/pubmed/26479967
http://dx.doi.org/10.1021/acs.bioconjchem.5b00504
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