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Expression partitioning of homeologs and tandem duplications contribute to salt tolerance in wheat (Triticum aestivum L.)
Salt stress dramatically reduces crop yield and quality, but the molecular mechanisms underlying salt tolerance remain largely unknown. To explore the wheat transcriptional response to salt stress, we performed high-throughput transcriptome sequencing of 10-day old wheat roots under normal condition...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759826/ https://www.ncbi.nlm.nih.gov/pubmed/26892368 http://dx.doi.org/10.1038/srep21476 |
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author | Zhang, Yumei Liu, Zhenshan Khan, Abul Awlad Lin, Qi Han, Yao Mu, Ping Liu, Yiguo Zhang, Hongsheng Li, Lingyan Meng, Xianghao Ni, Zhongfu Xin, Mingming |
author_facet | Zhang, Yumei Liu, Zhenshan Khan, Abul Awlad Lin, Qi Han, Yao Mu, Ping Liu, Yiguo Zhang, Hongsheng Li, Lingyan Meng, Xianghao Ni, Zhongfu Xin, Mingming |
author_sort | Zhang, Yumei |
collection | PubMed |
description | Salt stress dramatically reduces crop yield and quality, but the molecular mechanisms underlying salt tolerance remain largely unknown. To explore the wheat transcriptional response to salt stress, we performed high-throughput transcriptome sequencing of 10-day old wheat roots under normal condition and 6, 12, 24 and 48 h after salt stress (HASS) in both a salt-tolerant cultivar and salt-sensitive cultivar. The results demonstrated global gene expression reprogramming with 36,804 genes that were up- or down-regulated in wheat roots under at least one stress condition compared with the controls and revealed the specificity and complexity of the functional pathways between the two cultivars. Further analysis showed that substantial expression partitioning of homeologous wheat genes occurs when the plants are subjected to salt stress, accounting for approximately 63.9% (2,537) and 66.1% (2,624) of the homeologous genes in ‘Chinese Spring’ (CS) and ‘Qing Mai 6’ (QM). Interestingly, 143 salt-responsive genes have been duplicated and tandemly arrayed on chromosomes during wheat evolution and polyploidization events, and the expression patterns of 122 (122/143, 85.3%) tandem duplications diverged dynamically over the time-course of salinity exposure. In addition, constitutive expression or silencing of target genes in Arabidopsis and wheat further confirmed our high-confidence salt stress-responsive candidates. |
format | Online Article Text |
id | pubmed-4759826 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47598262016-02-29 Expression partitioning of homeologs and tandem duplications contribute to salt tolerance in wheat (Triticum aestivum L.) Zhang, Yumei Liu, Zhenshan Khan, Abul Awlad Lin, Qi Han, Yao Mu, Ping Liu, Yiguo Zhang, Hongsheng Li, Lingyan Meng, Xianghao Ni, Zhongfu Xin, Mingming Sci Rep Article Salt stress dramatically reduces crop yield and quality, but the molecular mechanisms underlying salt tolerance remain largely unknown. To explore the wheat transcriptional response to salt stress, we performed high-throughput transcriptome sequencing of 10-day old wheat roots under normal condition and 6, 12, 24 and 48 h after salt stress (HASS) in both a salt-tolerant cultivar and salt-sensitive cultivar. The results demonstrated global gene expression reprogramming with 36,804 genes that were up- or down-regulated in wheat roots under at least one stress condition compared with the controls and revealed the specificity and complexity of the functional pathways between the two cultivars. Further analysis showed that substantial expression partitioning of homeologous wheat genes occurs when the plants are subjected to salt stress, accounting for approximately 63.9% (2,537) and 66.1% (2,624) of the homeologous genes in ‘Chinese Spring’ (CS) and ‘Qing Mai 6’ (QM). Interestingly, 143 salt-responsive genes have been duplicated and tandemly arrayed on chromosomes during wheat evolution and polyploidization events, and the expression patterns of 122 (122/143, 85.3%) tandem duplications diverged dynamically over the time-course of salinity exposure. In addition, constitutive expression or silencing of target genes in Arabidopsis and wheat further confirmed our high-confidence salt stress-responsive candidates. Nature Publishing Group 2016-02-19 /pmc/articles/PMC4759826/ /pubmed/26892368 http://dx.doi.org/10.1038/srep21476 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Zhang, Yumei Liu, Zhenshan Khan, Abul Awlad Lin, Qi Han, Yao Mu, Ping Liu, Yiguo Zhang, Hongsheng Li, Lingyan Meng, Xianghao Ni, Zhongfu Xin, Mingming Expression partitioning of homeologs and tandem duplications contribute to salt tolerance in wheat (Triticum aestivum L.) |
title | Expression partitioning of homeologs and tandem duplications contribute to salt tolerance in wheat (Triticum aestivum L.) |
title_full | Expression partitioning of homeologs and tandem duplications contribute to salt tolerance in wheat (Triticum aestivum L.) |
title_fullStr | Expression partitioning of homeologs and tandem duplications contribute to salt tolerance in wheat (Triticum aestivum L.) |
title_full_unstemmed | Expression partitioning of homeologs and tandem duplications contribute to salt tolerance in wheat (Triticum aestivum L.) |
title_short | Expression partitioning of homeologs and tandem duplications contribute to salt tolerance in wheat (Triticum aestivum L.) |
title_sort | expression partitioning of homeologs and tandem duplications contribute to salt tolerance in wheat (triticum aestivum l.) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759826/ https://www.ncbi.nlm.nih.gov/pubmed/26892368 http://dx.doi.org/10.1038/srep21476 |
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