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Monitoring of extracellular pH in young dental biofilms grown in vivo in the presence and absence of sucrose
BACKGROUND AND OBJECTIVE: pH in dental biofilms is of central importance for the development of caries. We used the ratiometric pH-sensitive dye C-SNARF-4 in combination with digital image analysis to monitor extracellular pH in dental biofilms grown in situ with and without sucrose supply. DESIGN:...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Co-Action Publishing
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759832/ https://www.ncbi.nlm.nih.gov/pubmed/26894480 http://dx.doi.org/10.3402/jom.v8.30390 |
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author | Dige, Irene Baelum, Vibeke Nyvad, Bente Schlafer, Sebastian |
author_facet | Dige, Irene Baelum, Vibeke Nyvad, Bente Schlafer, Sebastian |
author_sort | Dige, Irene |
collection | PubMed |
description | BACKGROUND AND OBJECTIVE: pH in dental biofilms is of central importance for the development of caries. We used the ratiometric pH-sensitive dye C-SNARF-4 in combination with digital image analysis to monitor extracellular pH in dental biofilms grown in situ with and without sucrose supply. DESIGN: Dental biofilms (48 h) from 10 individuals were collected on glass slabs mounted on intra-oral appliances. During growth, appliances were immersed extra-orally in either physiological saline or 4% sucrose for 2 min, eight times per day. Fluorescence emissions of C-SNARF-4 in deep layers of the biofilms were recorded ex vivo with confocal microscopy for 15 min or for 1 h after exposure to 0.4% glucose. Extracellular pH was determined ratiometrically using digital image analysis. RESULTS: Extracellular pH dropped rapidly in most examined sites after addition of glucose. Distinct pH microenvironments were observed within single biofilms. The variation in pH was similar between sites within the same biofilm and sites from different individuals. pH drop patterns did not differ between biofilms exposed to sucrose-free and sucrose-rich environments. CONCLUSION: The present study is the first of its kind to apply the combination of pH ratiometry and digital image analysis to systematically record extracellular pH in intact dental biofilms from several individuals for up to 1 h. We observed highly heterogeneous pH landscapes and the presence of acidogenic microenvironments – ‘acidogenic hotspots’ – within the biofilms. The data suggest that pH drops in young (48 h) dental biofilms are independent of the sucrose supply during growth. |
format | Online Article Text |
id | pubmed-4759832 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Co-Action Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-47598322016-03-09 Monitoring of extracellular pH in young dental biofilms grown in vivo in the presence and absence of sucrose Dige, Irene Baelum, Vibeke Nyvad, Bente Schlafer, Sebastian J Oral Microbiol Original Article BACKGROUND AND OBJECTIVE: pH in dental biofilms is of central importance for the development of caries. We used the ratiometric pH-sensitive dye C-SNARF-4 in combination with digital image analysis to monitor extracellular pH in dental biofilms grown in situ with and without sucrose supply. DESIGN: Dental biofilms (48 h) from 10 individuals were collected on glass slabs mounted on intra-oral appliances. During growth, appliances were immersed extra-orally in either physiological saline or 4% sucrose for 2 min, eight times per day. Fluorescence emissions of C-SNARF-4 in deep layers of the biofilms were recorded ex vivo with confocal microscopy for 15 min or for 1 h after exposure to 0.4% glucose. Extracellular pH was determined ratiometrically using digital image analysis. RESULTS: Extracellular pH dropped rapidly in most examined sites after addition of glucose. Distinct pH microenvironments were observed within single biofilms. The variation in pH was similar between sites within the same biofilm and sites from different individuals. pH drop patterns did not differ between biofilms exposed to sucrose-free and sucrose-rich environments. CONCLUSION: The present study is the first of its kind to apply the combination of pH ratiometry and digital image analysis to systematically record extracellular pH in intact dental biofilms from several individuals for up to 1 h. We observed highly heterogeneous pH landscapes and the presence of acidogenic microenvironments – ‘acidogenic hotspots’ – within the biofilms. The data suggest that pH drops in young (48 h) dental biofilms are independent of the sucrose supply during growth. Co-Action Publishing 2016-02-15 /pmc/articles/PMC4759832/ /pubmed/26894480 http://dx.doi.org/10.3402/jom.v8.30390 Text en © 2016 Irene Dige et al. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Dige, Irene Baelum, Vibeke Nyvad, Bente Schlafer, Sebastian Monitoring of extracellular pH in young dental biofilms grown in vivo in the presence and absence of sucrose |
title | Monitoring of extracellular pH in young dental biofilms grown in vivo in the presence and absence of sucrose |
title_full | Monitoring of extracellular pH in young dental biofilms grown in vivo in the presence and absence of sucrose |
title_fullStr | Monitoring of extracellular pH in young dental biofilms grown in vivo in the presence and absence of sucrose |
title_full_unstemmed | Monitoring of extracellular pH in young dental biofilms grown in vivo in the presence and absence of sucrose |
title_short | Monitoring of extracellular pH in young dental biofilms grown in vivo in the presence and absence of sucrose |
title_sort | monitoring of extracellular ph in young dental biofilms grown in vivo in the presence and absence of sucrose |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4759832/ https://www.ncbi.nlm.nih.gov/pubmed/26894480 http://dx.doi.org/10.3402/jom.v8.30390 |
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