Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report

Within the EU-SPIDIA project (www.spidia.eu), the quality parameters of blood genomic DNA were defined [SPIDIA-DNA: an External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses – [1]; Influence of pre-analytical procedures on genomic DNA integrity in blood...

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Autores principales: Malentacchi, Francesca, Pizzamiglio, Sara, Ibrahim-Gawel, Hady, Pazzagli, Mario, Verderio, Paolo, Ciniselli, Chiara Maura, Wyrich, Ralf, Gelmini, Stefania
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4760182/
https://www.ncbi.nlm.nih.gov/pubmed/26949730
http://dx.doi.org/10.1016/j.dib.2016.01.062
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author Malentacchi, Francesca
Pizzamiglio, Sara
Ibrahim-Gawel, Hady
Pazzagli, Mario
Verderio, Paolo
Ciniselli, Chiara Maura
Wyrich, Ralf
Gelmini, Stefania
author_facet Malentacchi, Francesca
Pizzamiglio, Sara
Ibrahim-Gawel, Hady
Pazzagli, Mario
Verderio, Paolo
Ciniselli, Chiara Maura
Wyrich, Ralf
Gelmini, Stefania
author_sort Malentacchi, Francesca
collection PubMed
description Within the EU-SPIDIA project (www.spidia.eu), the quality parameters of blood genomic DNA were defined [SPIDIA-DNA: an External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses – [1]; Influence of pre-analytical procedures on genomic DNA integrity in blood samples: the SPIDIA experience – [2]; Combining qualitative and quantitative imaging evaluation for the assessment of genomic DNA integrity: the SPIDIA experience – [3]. DNA quality parameters were used to evaluate the laboratory performance within an External Quality Assessment (EQA) [Second SPIDIA-DNA External Quality Assessment (EQA): Influence of pre-analytical phase of blood samples on genomic DNA quality – [4]. These parameters included DNA purity and yield by UV spectrophotometric measurements, the presence of PCR interferences by Kineret software and genomic DNA integrity analysis by Pulsed Field Gel Electrophoresis. Here we present the specific laboratory report of the 2nd SPIDIA-DNA EQA as an example of data and performances evaluation.
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spelling pubmed-47601822016-03-04 Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report Malentacchi, Francesca Pizzamiglio, Sara Ibrahim-Gawel, Hady Pazzagli, Mario Verderio, Paolo Ciniselli, Chiara Maura Wyrich, Ralf Gelmini, Stefania Data Brief Data Article Within the EU-SPIDIA project (www.spidia.eu), the quality parameters of blood genomic DNA were defined [SPIDIA-DNA: an External Quality Assessment for the pre-analytical phase of blood samples used for DNA-based analyses – [1]; Influence of pre-analytical procedures on genomic DNA integrity in blood samples: the SPIDIA experience – [2]; Combining qualitative and quantitative imaging evaluation for the assessment of genomic DNA integrity: the SPIDIA experience – [3]. DNA quality parameters were used to evaluate the laboratory performance within an External Quality Assessment (EQA) [Second SPIDIA-DNA External Quality Assessment (EQA): Influence of pre-analytical phase of blood samples on genomic DNA quality – [4]. These parameters included DNA purity and yield by UV spectrophotometric measurements, the presence of PCR interferences by Kineret software and genomic DNA integrity analysis by Pulsed Field Gel Electrophoresis. Here we present the specific laboratory report of the 2nd SPIDIA-DNA EQA as an example of data and performances evaluation. Elsevier 2016-02-06 /pmc/articles/PMC4760182/ /pubmed/26949730 http://dx.doi.org/10.1016/j.dib.2016.01.062 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Malentacchi, Francesca
Pizzamiglio, Sara
Ibrahim-Gawel, Hady
Pazzagli, Mario
Verderio, Paolo
Ciniselli, Chiara Maura
Wyrich, Ralf
Gelmini, Stefania
Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report
title Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report
title_full Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report
title_fullStr Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report
title_full_unstemmed Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report
title_short Data and performances evaluation of the SPIDIA-DNA Pan-European External Quality Assessment: 2nd SPIDIA-DNA laboratory report
title_sort data and performances evaluation of the spidia-dna pan-european external quality assessment: 2nd spidia-dna laboratory report
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4760182/
https://www.ncbi.nlm.nih.gov/pubmed/26949730
http://dx.doi.org/10.1016/j.dib.2016.01.062
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