Cargando…

DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest(1)

PREMISE OF THE STUDY: Development and characterization of a set of DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a naturally rare and threatened tree species, were carried out to investigate its conservation genetics. METHODS AND RESULTS: Through an enriched genomic library procedu...

Descripción completa

Detalles Bibliográficos
Autores principales: Spoladore, Janaína, Mansano, Vidal F., Dias de Freitas, Luan C., Sebbenn, Alexandre M., Lemes, Maristerra R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4760745/
https://www.ncbi.nlm.nih.gov/pubmed/26949573
http://dx.doi.org/10.3732/apps.1500081
_version_ 1782416899862167552
author Spoladore, Janaína
Mansano, Vidal F.
Dias de Freitas, Luan C.
Sebbenn, Alexandre M.
Lemes, Maristerra R.
author_facet Spoladore, Janaína
Mansano, Vidal F.
Dias de Freitas, Luan C.
Sebbenn, Alexandre M.
Lemes, Maristerra R.
author_sort Spoladore, Janaína
collection PubMed
description PREMISE OF THE STUDY: Development and characterization of a set of DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a naturally rare and threatened tree species, were carried out to investigate its conservation genetics. METHODS AND RESULTS: Through an enriched genomic library procedure, 10 DNA microsatellite loci were isolated and characterized for the species. The mean expected heterozygosity was 0.776 (0.424–0.894). Cross-species amplifications of these loci were successfully tested for six congener taxa (S. apetala var. apetala, S. flaemingii, S. langsdorffii, S. macrostachya, S. myrtifolia var. elegans, and S. simplex var. continentalis). CONCLUSIONS: The 10 polymorphic microsatellite markers developed are quite informative and will provide a valuable resource to study the population and conservation genetics of S. glazioviana and other Swartzia species.
format Online
Article
Text
id pubmed-4760745
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Botanical Society of America
record_format MEDLINE/PubMed
spelling pubmed-47607452016-03-04 DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest(1) Spoladore, Janaína Mansano, Vidal F. Dias de Freitas, Luan C. Sebbenn, Alexandre M. Lemes, Maristerra R. Appl Plant Sci Primer Note PREMISE OF THE STUDY: Development and characterization of a set of DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a naturally rare and threatened tree species, were carried out to investigate its conservation genetics. METHODS AND RESULTS: Through an enriched genomic library procedure, 10 DNA microsatellite loci were isolated and characterized for the species. The mean expected heterozygosity was 0.776 (0.424–0.894). Cross-species amplifications of these loci were successfully tested for six congener taxa (S. apetala var. apetala, S. flaemingii, S. langsdorffii, S. macrostachya, S. myrtifolia var. elegans, and S. simplex var. continentalis). CONCLUSIONS: The 10 polymorphic microsatellite markers developed are quite informative and will provide a valuable resource to study the population and conservation genetics of S. glazioviana and other Swartzia species. Botanical Society of America 2016-02-11 /pmc/articles/PMC4760745/ /pubmed/26949573 http://dx.doi.org/10.3732/apps.1500081 Text en © 2016 Spoladore et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Spoladore, Janaína
Mansano, Vidal F.
Dias de Freitas, Luan C.
Sebbenn, Alexandre M.
Lemes, Maristerra R.
DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest(1)
title DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest(1)
title_full DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest(1)
title_fullStr DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest(1)
title_full_unstemmed DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest(1)
title_short DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest(1)
title_sort dna microsatellite markers for swartzia glazioviana (fabaceae), a threatened species from the brazilian atlantic forest(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4760745/
https://www.ncbi.nlm.nih.gov/pubmed/26949573
http://dx.doi.org/10.3732/apps.1500081
work_keys_str_mv AT spoladorejanaina dnamicrosatellitemarkersforswartziaglaziovianafabaceaeathreatenedspeciesfromthebrazilianatlanticforest1
AT mansanovidalf dnamicrosatellitemarkersforswartziaglaziovianafabaceaeathreatenedspeciesfromthebrazilianatlanticforest1
AT diasdefreitasluanc dnamicrosatellitemarkersforswartziaglaziovianafabaceaeathreatenedspeciesfromthebrazilianatlanticforest1
AT sebbennalexandrem dnamicrosatellitemarkersforswartziaglaziovianafabaceaeathreatenedspeciesfromthebrazilianatlanticforest1
AT lemesmaristerrar dnamicrosatellitemarkersforswartziaglaziovianafabaceaeathreatenedspeciesfromthebrazilianatlanticforest1