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DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest(1)
PREMISE OF THE STUDY: Development and characterization of a set of DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a naturally rare and threatened tree species, were carried out to investigate its conservation genetics. METHODS AND RESULTS: Through an enriched genomic library procedu...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Botanical Society of America
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4760745/ https://www.ncbi.nlm.nih.gov/pubmed/26949573 http://dx.doi.org/10.3732/apps.1500081 |
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author | Spoladore, Janaína Mansano, Vidal F. Dias de Freitas, Luan C. Sebbenn, Alexandre M. Lemes, Maristerra R. |
author_facet | Spoladore, Janaína Mansano, Vidal F. Dias de Freitas, Luan C. Sebbenn, Alexandre M. Lemes, Maristerra R. |
author_sort | Spoladore, Janaína |
collection | PubMed |
description | PREMISE OF THE STUDY: Development and characterization of a set of DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a naturally rare and threatened tree species, were carried out to investigate its conservation genetics. METHODS AND RESULTS: Through an enriched genomic library procedure, 10 DNA microsatellite loci were isolated and characterized for the species. The mean expected heterozygosity was 0.776 (0.424–0.894). Cross-species amplifications of these loci were successfully tested for six congener taxa (S. apetala var. apetala, S. flaemingii, S. langsdorffii, S. macrostachya, S. myrtifolia var. elegans, and S. simplex var. continentalis). CONCLUSIONS: The 10 polymorphic microsatellite markers developed are quite informative and will provide a valuable resource to study the population and conservation genetics of S. glazioviana and other Swartzia species. |
format | Online Article Text |
id | pubmed-4760745 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Botanical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-47607452016-03-04 DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest(1) Spoladore, Janaína Mansano, Vidal F. Dias de Freitas, Luan C. Sebbenn, Alexandre M. Lemes, Maristerra R. Appl Plant Sci Primer Note PREMISE OF THE STUDY: Development and characterization of a set of DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a naturally rare and threatened tree species, were carried out to investigate its conservation genetics. METHODS AND RESULTS: Through an enriched genomic library procedure, 10 DNA microsatellite loci were isolated and characterized for the species. The mean expected heterozygosity was 0.776 (0.424–0.894). Cross-species amplifications of these loci were successfully tested for six congener taxa (S. apetala var. apetala, S. flaemingii, S. langsdorffii, S. macrostachya, S. myrtifolia var. elegans, and S. simplex var. continentalis). CONCLUSIONS: The 10 polymorphic microsatellite markers developed are quite informative and will provide a valuable resource to study the population and conservation genetics of S. glazioviana and other Swartzia species. Botanical Society of America 2016-02-11 /pmc/articles/PMC4760745/ /pubmed/26949573 http://dx.doi.org/10.3732/apps.1500081 Text en © 2016 Spoladore et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA). |
spellingShingle | Primer Note Spoladore, Janaína Mansano, Vidal F. Dias de Freitas, Luan C. Sebbenn, Alexandre M. Lemes, Maristerra R. DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest(1) |
title | DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest(1) |
title_full | DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest(1) |
title_fullStr | DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest(1) |
title_full_unstemmed | DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest(1) |
title_short | DNA microsatellite markers for Swartzia glazioviana (Fabaceae), a threatened species from the Brazilian Atlantic Forest(1) |
title_sort | dna microsatellite markers for swartzia glazioviana (fabaceae), a threatened species from the brazilian atlantic forest(1) |
topic | Primer Note |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4760745/ https://www.ncbi.nlm.nih.gov/pubmed/26949573 http://dx.doi.org/10.3732/apps.1500081 |
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