Development of microsatellite markers for the semi-natural grassland herb Veronicastrum japonicum (Plantaginaceae)(1)

PREMISE OF THE STUDY: Veronicastrum japonicum (Plantaginaceae) grows in grasslands on Honshu Island, Japan, and is threatened by habitat loss because of rapid land development over recent decades. For the genetic characterization of the remaining populations, microsatellite markers were developed. M...

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Autores principales: Nakahama, Naoyuki, Izuno, Ayako, Arima, Kurumi, Isagi, Yuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2016
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4760747/
https://www.ncbi.nlm.nih.gov/pubmed/26949575
http://dx.doi.org/10.3732/apps.1500096
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author Nakahama, Naoyuki
Izuno, Ayako
Arima, Kurumi
Isagi, Yuji
author_facet Nakahama, Naoyuki
Izuno, Ayako
Arima, Kurumi
Isagi, Yuji
author_sort Nakahama, Naoyuki
collection PubMed
description PREMISE OF THE STUDY: Veronicastrum japonicum (Plantaginaceae) grows in grasslands on Honshu Island, Japan, and is threatened by habitat loss because of rapid land development over recent decades. For the genetic characterization of the remaining populations, microsatellite markers were developed. METHODS AND RESULTS: Twelve polymorphic microsatellite loci were developed using next-generation sequencing. The number of alleles per locus ranged from two to 24 (mean 7.7), and the expected heterozygosity per locus ranged from 0.35 to 0.94 (mean 0.68). CONCLUSIONS: These markers can be used for genetic studies in conservation, such as the evaluation of genetic diversity and genetic structure.
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spelling pubmed-47607472016-03-04 Development of microsatellite markers for the semi-natural grassland herb Veronicastrum japonicum (Plantaginaceae)(1) Nakahama, Naoyuki Izuno, Ayako Arima, Kurumi Isagi, Yuji Appl Plant Sci Primer Note PREMISE OF THE STUDY: Veronicastrum japonicum (Plantaginaceae) grows in grasslands on Honshu Island, Japan, and is threatened by habitat loss because of rapid land development over recent decades. For the genetic characterization of the remaining populations, microsatellite markers were developed. METHODS AND RESULTS: Twelve polymorphic microsatellite loci were developed using next-generation sequencing. The number of alleles per locus ranged from two to 24 (mean 7.7), and the expected heterozygosity per locus ranged from 0.35 to 0.94 (mean 0.68). CONCLUSIONS: These markers can be used for genetic studies in conservation, such as the evaluation of genetic diversity and genetic structure. Botanical Society of America 2016-02-11 /pmc/articles/PMC4760747/ /pubmed/26949575 http://dx.doi.org/10.3732/apps.1500096 Text en © 2016 Nakahama et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Nakahama, Naoyuki
Izuno, Ayako
Arima, Kurumi
Isagi, Yuji
Development of microsatellite markers for the semi-natural grassland herb Veronicastrum japonicum (Plantaginaceae)(1)
title Development of microsatellite markers for the semi-natural grassland herb Veronicastrum japonicum (Plantaginaceae)(1)
title_full Development of microsatellite markers for the semi-natural grassland herb Veronicastrum japonicum (Plantaginaceae)(1)
title_fullStr Development of microsatellite markers for the semi-natural grassland herb Veronicastrum japonicum (Plantaginaceae)(1)
title_full_unstemmed Development of microsatellite markers for the semi-natural grassland herb Veronicastrum japonicum (Plantaginaceae)(1)
title_short Development of microsatellite markers for the semi-natural grassland herb Veronicastrum japonicum (Plantaginaceae)(1)
title_sort development of microsatellite markers for the semi-natural grassland herb veronicastrum japonicum (plantaginaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4760747/
https://www.ncbi.nlm.nih.gov/pubmed/26949575
http://dx.doi.org/10.3732/apps.1500096
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