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Depot-Specific Changes in Fat Metabolism with Aging in a Type 2 Diabetic Animal Model
Visceral fat accretion is a hallmark of aging and is associated with aging-induced metabolic dysfunction. PPARγ agonist was reported to improve insulin sensitivity by redistributing fat from visceral fat to subcutaneous fat. The purpose of this study was to investigate the underlying mechanisms by w...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4760935/ https://www.ncbi.nlm.nih.gov/pubmed/26894429 http://dx.doi.org/10.1371/journal.pone.0148141 |
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author | Park, Se Eun Park, Cheol-Young Choi, Jung Mook Chang, Eugene Rhee, Eun-Jung Lee, Won-Young Oh, Ki Won Park, Sung Woo Kang, Eun Seok Lee, Hyun Chul Cha, Bong Soo |
author_facet | Park, Se Eun Park, Cheol-Young Choi, Jung Mook Chang, Eugene Rhee, Eun-Jung Lee, Won-Young Oh, Ki Won Park, Sung Woo Kang, Eun Seok Lee, Hyun Chul Cha, Bong Soo |
author_sort | Park, Se Eun |
collection | PubMed |
description | Visceral fat accretion is a hallmark of aging and is associated with aging-induced metabolic dysfunction. PPARγ agonist was reported to improve insulin sensitivity by redistributing fat from visceral fat to subcutaneous fat. The purpose of this study was to investigate the underlying mechanisms by which aging affects adipose tissue remodeling in a type 2 diabetic animal model and through which PPARγ activation modulates aging-related fat tissue distribution. At the ages of 21, 31 and 43 weeks, OLETF rats as an animal model of type 2 diabetes were evaluated for aging-related effects on adipose tissue metabolism in subcutaneous and visceral fat depots. During aging, the ratio of visceral fat weight to subcutaneous fat weight (V/S ratio) increased. Aging significantly increased the mRNA expression of genes involved in lipogenesis such as lipoprotein lipase, fatty acid binding protein aP2, lipin 1, and diacylglycerol acyltransferase 1, which were more prominent in visceral fat than subcutaneous fat. The mRNA expression of adipose triglyceride lipase, which is involved in basal lipolysis and fatty acid recycling, was also increased, more in visceral fat compared to subcutaneous fat during aging. The mRNA levels of the genes associated with lipid oxidation were increased, whereas the mRNA levels of genes associated with energy expenditure showed no significant change during aging. PPARγ agonist treatment in OLETF rats resulted in fat redistribution with a decreasing V/S ratio and improved glucose intolerance. The genes involved in lipogenesis decreased in visceral fat of the PPARγ agonist-treated rats. During aging, fat distribution was changed by stimulating lipid uptake and esterification in visceral fat rather than subcutaneous fat, and by altering the lipid oxidation. |
format | Online Article Text |
id | pubmed-4760935 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47609352016-03-07 Depot-Specific Changes in Fat Metabolism with Aging in a Type 2 Diabetic Animal Model Park, Se Eun Park, Cheol-Young Choi, Jung Mook Chang, Eugene Rhee, Eun-Jung Lee, Won-Young Oh, Ki Won Park, Sung Woo Kang, Eun Seok Lee, Hyun Chul Cha, Bong Soo PLoS One Research Article Visceral fat accretion is a hallmark of aging and is associated with aging-induced metabolic dysfunction. PPARγ agonist was reported to improve insulin sensitivity by redistributing fat from visceral fat to subcutaneous fat. The purpose of this study was to investigate the underlying mechanisms by which aging affects adipose tissue remodeling in a type 2 diabetic animal model and through which PPARγ activation modulates aging-related fat tissue distribution. At the ages of 21, 31 and 43 weeks, OLETF rats as an animal model of type 2 diabetes were evaluated for aging-related effects on adipose tissue metabolism in subcutaneous and visceral fat depots. During aging, the ratio of visceral fat weight to subcutaneous fat weight (V/S ratio) increased. Aging significantly increased the mRNA expression of genes involved in lipogenesis such as lipoprotein lipase, fatty acid binding protein aP2, lipin 1, and diacylglycerol acyltransferase 1, which were more prominent in visceral fat than subcutaneous fat. The mRNA expression of adipose triglyceride lipase, which is involved in basal lipolysis and fatty acid recycling, was also increased, more in visceral fat compared to subcutaneous fat during aging. The mRNA levels of the genes associated with lipid oxidation were increased, whereas the mRNA levels of genes associated with energy expenditure showed no significant change during aging. PPARγ agonist treatment in OLETF rats resulted in fat redistribution with a decreasing V/S ratio and improved glucose intolerance. The genes involved in lipogenesis decreased in visceral fat of the PPARγ agonist-treated rats. During aging, fat distribution was changed by stimulating lipid uptake and esterification in visceral fat rather than subcutaneous fat, and by altering the lipid oxidation. Public Library of Science 2016-02-19 /pmc/articles/PMC4760935/ /pubmed/26894429 http://dx.doi.org/10.1371/journal.pone.0148141 Text en © 2016 Park et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Park, Se Eun Park, Cheol-Young Choi, Jung Mook Chang, Eugene Rhee, Eun-Jung Lee, Won-Young Oh, Ki Won Park, Sung Woo Kang, Eun Seok Lee, Hyun Chul Cha, Bong Soo Depot-Specific Changes in Fat Metabolism with Aging in a Type 2 Diabetic Animal Model |
title | Depot-Specific Changes in Fat Metabolism with Aging in a Type 2 Diabetic Animal Model |
title_full | Depot-Specific Changes in Fat Metabolism with Aging in a Type 2 Diabetic Animal Model |
title_fullStr | Depot-Specific Changes in Fat Metabolism with Aging in a Type 2 Diabetic Animal Model |
title_full_unstemmed | Depot-Specific Changes in Fat Metabolism with Aging in a Type 2 Diabetic Animal Model |
title_short | Depot-Specific Changes in Fat Metabolism with Aging in a Type 2 Diabetic Animal Model |
title_sort | depot-specific changes in fat metabolism with aging in a type 2 diabetic animal model |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4760935/ https://www.ncbi.nlm.nih.gov/pubmed/26894429 http://dx.doi.org/10.1371/journal.pone.0148141 |
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