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A high-throughput method for genotyping S-RNase alleles in apple
We present a new efficient screening tool for detection of S-alleles in apple. The protocol using general and multiplexed primers for PCR reaction and fragment detection on an automatized capillary DNA sequencer exposed a higher number of alleles than any previous studies. Analysis of alleles is mad...
Autores principales: | Larsen, Bjarne, Ørgaard, Marian, Toldam-Andersen, Torben Bo, Pedersen, Carsten |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4760992/ https://www.ncbi.nlm.nih.gov/pubmed/26941563 http://dx.doi.org/10.1007/s11032-016-0448-0 |
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