Molecular detection of Mycobacterium bovis in cattle herds of the state of Pernambuco, Brazil

BACKGROUND: The present study aimed to direct detect Mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20 properties located in the state of Pernambuco, Brazil, by real-time quantitative PCR (qPCR) targeting the region of difference 4 (RD4). Risk fact...

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Detalles Bibliográficos
Autores principales: Cezar, Renata Duarte da Silva, Lucena-Silva, Norma, Filho, Antônio Fernando Barbosa Batista, Borges, Jonas de Melo, de Oliveira, Pollyane Raysa Fernandes, Lúcio, Érica Chaves, Arruda-Lima, Maíra, Santana, Vania Lucia de Assis, Pinheiro Junior, José Wilton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761206/
https://www.ncbi.nlm.nih.gov/pubmed/26897524
http://dx.doi.org/10.1186/s12917-016-0656-1
Descripción
Sumario:BACKGROUND: The present study aimed to direct detect Mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20 properties located in the state of Pernambuco, Brazil, by real-time quantitative PCR (qPCR) targeting the region of difference 4 (RD4). Risk factors possibly associated with bovine tuberculosis (BTB) were also evaluated. RESULTS: Of the 802 samples analyzed, one milk (0.25 %) and eight blood (2 %) samples were positive for M. bovis in the qPCR and their identities were confirmed by sequencing. Animals positive for M. bovis were found in six (30 %) of the 20 properties visited. None of the risk factors evaluated were statistically associated with BTB. CONCLUSIONS: M. bovis DNA was detected in one milk sample what may pose a risk to public health because raw milk is commonly consumed in Brazil.

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