Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization

Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which es...

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Detalles Bibliográficos
Autores principales: Church, Molly E., Dela Cruz, Florante N., Kim, Kevin, Persiani, Michele, Woods, Leslie W., Pesavento, Patricia A., Woolard, Kevin D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761659/
https://www.ncbi.nlm.nih.gov/pubmed/26955649
http://dx.doi.org/10.1016/j.dib.2016.01.070
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author Church, Molly E.
Dela Cruz, Florante N.
Kim, Kevin
Persiani, Michele
Woods, Leslie W.
Pesavento, Patricia A.
Woolard, Kevin D.
author_facet Church, Molly E.
Dela Cruz, Florante N.
Kim, Kevin
Persiani, Michele
Woods, Leslie W.
Pesavento, Patricia A.
Woolard, Kevin D.
author_sort Church, Molly E.
collection PubMed
description Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which established the extent of exposure of free ranging raccoons to a newly described polyomavirus (RacPyV) [1]. Production of a polyclonal antibody has allowed for further characterization of RacPyV, including immunohistochemistry and immunocytochemistry techniques, in order to answer questions about pathogenesis of this virus.
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spelling pubmed-47616592016-03-07 Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization Church, Molly E. Dela Cruz, Florante N. Kim, Kevin Persiani, Michele Woods, Leslie W. Pesavento, Patricia A. Woolard, Kevin D. Data Brief Data Article Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which established the extent of exposure of free ranging raccoons to a newly described polyomavirus (RacPyV) [1]. Production of a polyclonal antibody has allowed for further characterization of RacPyV, including immunohistochemistry and immunocytochemistry techniques, in order to answer questions about pathogenesis of this virus. Elsevier 2016-02-09 /pmc/articles/PMC4761659/ /pubmed/26955649 http://dx.doi.org/10.1016/j.dib.2016.01.070 Text en © 2016 Published by Elsevier Inc. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Church, Molly E.
Dela Cruz, Florante N.
Kim, Kevin
Persiani, Michele
Woods, Leslie W.
Pesavento, Patricia A.
Woolard, Kevin D.
Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization
title Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization
title_full Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization
title_fullStr Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization
title_full_unstemmed Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization
title_short Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization
title_sort production of a recombinant capsid protein vp1 from a newly described polyomavirus (racpyv) for downstream use in virus characterization
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761659/
https://www.ncbi.nlm.nih.gov/pubmed/26955649
http://dx.doi.org/10.1016/j.dib.2016.01.070
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