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Production of a recombinant capsid protein VP1 from a newly described polyomavirus (RacPyV) for downstream use in virus characterization
Here we describe the methods for production of a recombinant viral capsid protein and subsequent use in an indirect enzyme linked immunosorbent assay (ELISA), and for use in production of a rabbit polyclonal antibody. These reagents were utilized in development and optimization of an ELISA, which es...
Autores principales: | Church, Molly E., Dela Cruz, Florante N., Kim, Kevin, Persiani, Michele, Woods, Leslie W., Pesavento, Patricia A., Woolard, Kevin D. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761659/ https://www.ncbi.nlm.nih.gov/pubmed/26955649 http://dx.doi.org/10.1016/j.dib.2016.01.070 |
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