Cargando…

Inhibitory effects and molecular mechanisms of tetrahydrocurcumin against human breast cancer MCF-7 cells

BACKGROUND: Tetrahydrocurcumin (THC), an active metabolite of curcumin, has been reported to have similar biological effects to curcumin, but the mechanism of the antitumor activity of THC is still unclear. METHODS: The present study was to investigate the antitumor effects and mechanism of THC in h...

Descripción completa

Detalles Bibliográficos
Autores principales: Han, Xiao, Deng, Shan, Wang, Ning, Liu, Yafei, Yang, Xingbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Co-Action Publishing 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761686/
https://www.ncbi.nlm.nih.gov/pubmed/26899573
http://dx.doi.org/10.3402/fnr.v60.30616
_version_ 1782416998564626432
author Han, Xiao
Deng, Shan
Wang, Ning
Liu, Yafei
Yang, Xingbin
author_facet Han, Xiao
Deng, Shan
Wang, Ning
Liu, Yafei
Yang, Xingbin
author_sort Han, Xiao
collection PubMed
description BACKGROUND: Tetrahydrocurcumin (THC), an active metabolite of curcumin, has been reported to have similar biological effects to curcumin, but the mechanism of the antitumor activity of THC is still unclear. METHODS: The present study was to investigate the antitumor effects and mechanism of THC in human breast cancer MCF-7 cells using the methods of MTT assay, LDH assay, flow cytometry analysis, and western blot assay. RESULTS: THC was found to have markedly cytotoxic effect and antiproliferative activity against MCF-7 cells in a dose-dependent manner with the IC(50) for 24 h of 107.8 μM. Flow cytometry analysis revealed that THC mediated the cell-cycle arrest at G0/G1 phase, and 32.8% of MCF-7 cells entered the early phase of apoptosis at 100 μM for 24 h. THC also dose-dependently led to apoptosis in MCF-7 cells via the mitochondrial pathway, as evidenced by the activation of caspase-3 and caspase-9, the elevation of intracellular ROS, a decrease in Bcl-2 and PARP expression, and an increase in Bax expression. Meanwhile, cytochrome C was released to cytosol and the loss of mitochondria membrane potential (Δψm) was observed after THC treatment. CONCLUSION: THC is an excellent source of chemopreventive agents in the treatment of breast cancer and has excellent potential to be explored as antitumor precursor compound.
format Online
Article
Text
id pubmed-4761686
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Co-Action Publishing
record_format MEDLINE/PubMed
spelling pubmed-47616862016-03-09 Inhibitory effects and molecular mechanisms of tetrahydrocurcumin against human breast cancer MCF-7 cells Han, Xiao Deng, Shan Wang, Ning Liu, Yafei Yang, Xingbin Food Nutr Res Original Article BACKGROUND: Tetrahydrocurcumin (THC), an active metabolite of curcumin, has been reported to have similar biological effects to curcumin, but the mechanism of the antitumor activity of THC is still unclear. METHODS: The present study was to investigate the antitumor effects and mechanism of THC in human breast cancer MCF-7 cells using the methods of MTT assay, LDH assay, flow cytometry analysis, and western blot assay. RESULTS: THC was found to have markedly cytotoxic effect and antiproliferative activity against MCF-7 cells in a dose-dependent manner with the IC(50) for 24 h of 107.8 μM. Flow cytometry analysis revealed that THC mediated the cell-cycle arrest at G0/G1 phase, and 32.8% of MCF-7 cells entered the early phase of apoptosis at 100 μM for 24 h. THC also dose-dependently led to apoptosis in MCF-7 cells via the mitochondrial pathway, as evidenced by the activation of caspase-3 and caspase-9, the elevation of intracellular ROS, a decrease in Bcl-2 and PARP expression, and an increase in Bax expression. Meanwhile, cytochrome C was released to cytosol and the loss of mitochondria membrane potential (Δψm) was observed after THC treatment. CONCLUSION: THC is an excellent source of chemopreventive agents in the treatment of breast cancer and has excellent potential to be explored as antitumor precursor compound. Co-Action Publishing 2016-02-17 /pmc/articles/PMC4761686/ /pubmed/26899573 http://dx.doi.org/10.3402/fnr.v60.30616 Text en © 2016 Xiao Han et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 International License, allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.
spellingShingle Original Article
Han, Xiao
Deng, Shan
Wang, Ning
Liu, Yafei
Yang, Xingbin
Inhibitory effects and molecular mechanisms of tetrahydrocurcumin against human breast cancer MCF-7 cells
title Inhibitory effects and molecular mechanisms of tetrahydrocurcumin against human breast cancer MCF-7 cells
title_full Inhibitory effects and molecular mechanisms of tetrahydrocurcumin against human breast cancer MCF-7 cells
title_fullStr Inhibitory effects and molecular mechanisms of tetrahydrocurcumin against human breast cancer MCF-7 cells
title_full_unstemmed Inhibitory effects and molecular mechanisms of tetrahydrocurcumin against human breast cancer MCF-7 cells
title_short Inhibitory effects and molecular mechanisms of tetrahydrocurcumin against human breast cancer MCF-7 cells
title_sort inhibitory effects and molecular mechanisms of tetrahydrocurcumin against human breast cancer mcf-7 cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761686/
https://www.ncbi.nlm.nih.gov/pubmed/26899573
http://dx.doi.org/10.3402/fnr.v60.30616
work_keys_str_mv AT hanxiao inhibitoryeffectsandmolecularmechanismsoftetrahydrocurcuminagainsthumanbreastcancermcf7cells
AT dengshan inhibitoryeffectsandmolecularmechanismsoftetrahydrocurcuminagainsthumanbreastcancermcf7cells
AT wangning inhibitoryeffectsandmolecularmechanismsoftetrahydrocurcuminagainsthumanbreastcancermcf7cells
AT liuyafei inhibitoryeffectsandmolecularmechanismsoftetrahydrocurcuminagainsthumanbreastcancermcf7cells
AT yangxingbin inhibitoryeffectsandmolecularmechanismsoftetrahydrocurcuminagainsthumanbreastcancermcf7cells