Mammalian interspecies substitution of immune modulatory alleles by genome editing

We describe a fundamentally novel feat of animal genetic engineering: the precise and efficient substitution of an agronomic haplotype into a domesticated species. Zinc finger nuclease in-embryo editing of the RELA locus generated live born domestic pigs with the warthog RELA orthologue, associated...

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Detalles Bibliográficos
Autores principales: Lillico, Simon G., Proudfoot, Chris, King, Tim J., Tan, Wenfang, Zhang, Lei, Mardjuki, Rachel, Paschon, David E., Rebar, Edward J., Urnov, Fyodor D., Mileham, Alan J., McLaren, David G., Whitelaw, C. Bruce A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761920/
https://www.ncbi.nlm.nih.gov/pubmed/26898342
http://dx.doi.org/10.1038/srep21645
Descripción
Sumario:We describe a fundamentally novel feat of animal genetic engineering: the precise and efficient substitution of an agronomic haplotype into a domesticated species. Zinc finger nuclease in-embryo editing of the RELA locus generated live born domestic pigs with the warthog RELA orthologue, associated with resilience to African Swine Fever. The ability to efficiently achieve interspecies allele introgression in one generation opens unprecedented opportunities for agriculture and basic research.

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