Mammalian interspecies substitution of immune modulatory alleles by genome editing

We describe a fundamentally novel feat of animal genetic engineering: the precise and efficient substitution of an agronomic haplotype into a domesticated species. Zinc finger nuclease in-embryo editing of the RELA locus generated live born domestic pigs with the warthog RELA orthologue, associated...

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Autores principales: Lillico, Simon G., Proudfoot, Chris, King, Tim J., Tan, Wenfang, Zhang, Lei, Mardjuki, Rachel, Paschon, David E., Rebar, Edward J., Urnov, Fyodor D., Mileham, Alan J., McLaren, David G., Whitelaw, C. Bruce A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761920/
https://www.ncbi.nlm.nih.gov/pubmed/26898342
http://dx.doi.org/10.1038/srep21645
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author Lillico, Simon G.
Proudfoot, Chris
King, Tim J.
Tan, Wenfang
Zhang, Lei
Mardjuki, Rachel
Paschon, David E.
Rebar, Edward J.
Urnov, Fyodor D.
Mileham, Alan J.
McLaren, David G.
Whitelaw, C. Bruce A.
author_facet Lillico, Simon G.
Proudfoot, Chris
King, Tim J.
Tan, Wenfang
Zhang, Lei
Mardjuki, Rachel
Paschon, David E.
Rebar, Edward J.
Urnov, Fyodor D.
Mileham, Alan J.
McLaren, David G.
Whitelaw, C. Bruce A.
author_sort Lillico, Simon G.
collection PubMed
description We describe a fundamentally novel feat of animal genetic engineering: the precise and efficient substitution of an agronomic haplotype into a domesticated species. Zinc finger nuclease in-embryo editing of the RELA locus generated live born domestic pigs with the warthog RELA orthologue, associated with resilience to African Swine Fever. The ability to efficiently achieve interspecies allele introgression in one generation opens unprecedented opportunities for agriculture and basic research.
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spelling pubmed-47619202016-02-29 Mammalian interspecies substitution of immune modulatory alleles by genome editing Lillico, Simon G. Proudfoot, Chris King, Tim J. Tan, Wenfang Zhang, Lei Mardjuki, Rachel Paschon, David E. Rebar, Edward J. Urnov, Fyodor D. Mileham, Alan J. McLaren, David G. Whitelaw, C. Bruce A. Sci Rep Article We describe a fundamentally novel feat of animal genetic engineering: the precise and efficient substitution of an agronomic haplotype into a domesticated species. Zinc finger nuclease in-embryo editing of the RELA locus generated live born domestic pigs with the warthog RELA orthologue, associated with resilience to African Swine Fever. The ability to efficiently achieve interspecies allele introgression in one generation opens unprecedented opportunities for agriculture and basic research. Nature Publishing Group 2016-02-22 /pmc/articles/PMC4761920/ /pubmed/26898342 http://dx.doi.org/10.1038/srep21645 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lillico, Simon G.
Proudfoot, Chris
King, Tim J.
Tan, Wenfang
Zhang, Lei
Mardjuki, Rachel
Paschon, David E.
Rebar, Edward J.
Urnov, Fyodor D.
Mileham, Alan J.
McLaren, David G.
Whitelaw, C. Bruce A.
Mammalian interspecies substitution of immune modulatory alleles by genome editing
title Mammalian interspecies substitution of immune modulatory alleles by genome editing
title_full Mammalian interspecies substitution of immune modulatory alleles by genome editing
title_fullStr Mammalian interspecies substitution of immune modulatory alleles by genome editing
title_full_unstemmed Mammalian interspecies substitution of immune modulatory alleles by genome editing
title_short Mammalian interspecies substitution of immune modulatory alleles by genome editing
title_sort mammalian interspecies substitution of immune modulatory alleles by genome editing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761920/
https://www.ncbi.nlm.nih.gov/pubmed/26898342
http://dx.doi.org/10.1038/srep21645
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