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Computer-aided multiple-head 3D printing system for printing of heterogeneous organ/tissue constructs
Recently, much attention has focused on replacement or/and enhancement of biological tissues via the use of cell-laden hydrogel scaffolds with an architecture that mimics the tissue matrix, and with the desired three-dimensional (3D) external geometry. However, mimicking the heterogeneous tissues th...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761951/ https://www.ncbi.nlm.nih.gov/pubmed/26899876 http://dx.doi.org/10.1038/srep21685 |
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author | Jung, Jin Woo Lee, Jung-Seob Cho, Dong-Woo |
author_facet | Jung, Jin Woo Lee, Jung-Seob Cho, Dong-Woo |
author_sort | Jung, Jin Woo |
collection | PubMed |
description | Recently, much attention has focused on replacement or/and enhancement of biological tissues via the use of cell-laden hydrogel scaffolds with an architecture that mimics the tissue matrix, and with the desired three-dimensional (3D) external geometry. However, mimicking the heterogeneous tissues that most organs and tissues are formed of is challenging. Although multiple-head 3D printing systems have been proposed for fabricating heterogeneous cell-laden hydrogel scaffolds, to date only the simple exterior form has been realized. Here we describe a computer-aided design and manufacturing (CAD/CAM) system for this application. We aim to develop an algorithm to enable easy, intuitive design and fabrication of a heterogeneous cell-laden hydrogel scaffolds with a free-form 3D geometry. The printing paths of the scaffold are automatically generated from the 3D CAD model, and the scaffold is then printed by dispensing four materials; i.e., a frame, two kinds of cell-laden hydrogel and a support. We demonstrated printing of heterogeneous tissue models formed of hydrogel scaffolds using this approach, including the outer ear, kidney and tooth tissue. These results indicate that this approach is particularly promising for tissue engineering and 3D printing applications to regenerate heterogeneous organs and tissues with tailored geometries to treat specific defects or injuries. |
format | Online Article Text |
id | pubmed-4761951 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47619512016-02-29 Computer-aided multiple-head 3D printing system for printing of heterogeneous organ/tissue constructs Jung, Jin Woo Lee, Jung-Seob Cho, Dong-Woo Sci Rep Article Recently, much attention has focused on replacement or/and enhancement of biological tissues via the use of cell-laden hydrogel scaffolds with an architecture that mimics the tissue matrix, and with the desired three-dimensional (3D) external geometry. However, mimicking the heterogeneous tissues that most organs and tissues are formed of is challenging. Although multiple-head 3D printing systems have been proposed for fabricating heterogeneous cell-laden hydrogel scaffolds, to date only the simple exterior form has been realized. Here we describe a computer-aided design and manufacturing (CAD/CAM) system for this application. We aim to develop an algorithm to enable easy, intuitive design and fabrication of a heterogeneous cell-laden hydrogel scaffolds with a free-form 3D geometry. The printing paths of the scaffold are automatically generated from the 3D CAD model, and the scaffold is then printed by dispensing four materials; i.e., a frame, two kinds of cell-laden hydrogel and a support. We demonstrated printing of heterogeneous tissue models formed of hydrogel scaffolds using this approach, including the outer ear, kidney and tooth tissue. These results indicate that this approach is particularly promising for tissue engineering and 3D printing applications to regenerate heterogeneous organs and tissues with tailored geometries to treat specific defects or injuries. Nature Publishing Group 2016-02-22 /pmc/articles/PMC4761951/ /pubmed/26899876 http://dx.doi.org/10.1038/srep21685 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Jung, Jin Woo Lee, Jung-Seob Cho, Dong-Woo Computer-aided multiple-head 3D printing system for printing of heterogeneous organ/tissue constructs |
title | Computer-aided multiple-head 3D printing system for printing of heterogeneous organ/tissue constructs |
title_full | Computer-aided multiple-head 3D printing system for printing of heterogeneous organ/tissue constructs |
title_fullStr | Computer-aided multiple-head 3D printing system for printing of heterogeneous organ/tissue constructs |
title_full_unstemmed | Computer-aided multiple-head 3D printing system for printing of heterogeneous organ/tissue constructs |
title_short | Computer-aided multiple-head 3D printing system for printing of heterogeneous organ/tissue constructs |
title_sort | computer-aided multiple-head 3d printing system for printing of heterogeneous organ/tissue constructs |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761951/ https://www.ncbi.nlm.nih.gov/pubmed/26899876 http://dx.doi.org/10.1038/srep21685 |
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