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PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes
PAX6 is the key transcription factor involved in eye development in humans, but the differential functions of the two PAX6 isoforms, isoform-a and isoform-b, are largely unknown. To reveal their function in the corneal epithelium, PAX6 isoforms, along with reprogramming factors, were transduced into...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761963/ https://www.ncbi.nlm.nih.gov/pubmed/26899008 http://dx.doi.org/10.1038/srep20807 |
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author | Sasamoto, Yuzuru Hayashi, Ryuhei Park, Sung-Joon Saito-Adachi, Mihoko Suzuki, Yutaka Kawasaki, Satoshi Quantock, Andrew J. Nakai, Kenta Tsujikawa, Motokazu Nishida, Kohji |
author_facet | Sasamoto, Yuzuru Hayashi, Ryuhei Park, Sung-Joon Saito-Adachi, Mihoko Suzuki, Yutaka Kawasaki, Satoshi Quantock, Andrew J. Nakai, Kenta Tsujikawa, Motokazu Nishida, Kohji |
author_sort | Sasamoto, Yuzuru |
collection | PubMed |
description | PAX6 is the key transcription factor involved in eye development in humans, but the differential functions of the two PAX6 isoforms, isoform-a and isoform-b, are largely unknown. To reveal their function in the corneal epithelium, PAX6 isoforms, along with reprogramming factors, were transduced into human non-ocular epithelial cells. Herein, we show that the two PAX6 isoforms differentially and cooperatively regulate the expression of genes specific to the structure and functions of the corneal epithelium, particularly keratin 3 (KRT3) and keratin 12 (KRT12). PAX6 isoform-a induced KRT3 expression by targeting its upstream region. KLF4 enhanced this induction. A combination of PAX6 isoform-b, KLF4, and OCT4 induced KRT12 expression. These new findings will contribute to furthering the understanding of the molecular basis of the corneal epithelium specific phenotype. |
format | Online Article Text |
id | pubmed-4761963 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47619632016-02-29 PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes Sasamoto, Yuzuru Hayashi, Ryuhei Park, Sung-Joon Saito-Adachi, Mihoko Suzuki, Yutaka Kawasaki, Satoshi Quantock, Andrew J. Nakai, Kenta Tsujikawa, Motokazu Nishida, Kohji Sci Rep Article PAX6 is the key transcription factor involved in eye development in humans, but the differential functions of the two PAX6 isoforms, isoform-a and isoform-b, are largely unknown. To reveal their function in the corneal epithelium, PAX6 isoforms, along with reprogramming factors, were transduced into human non-ocular epithelial cells. Herein, we show that the two PAX6 isoforms differentially and cooperatively regulate the expression of genes specific to the structure and functions of the corneal epithelium, particularly keratin 3 (KRT3) and keratin 12 (KRT12). PAX6 isoform-a induced KRT3 expression by targeting its upstream region. KLF4 enhanced this induction. A combination of PAX6 isoform-b, KLF4, and OCT4 induced KRT12 expression. These new findings will contribute to furthering the understanding of the molecular basis of the corneal epithelium specific phenotype. Nature Publishing Group 2016-02-22 /pmc/articles/PMC4761963/ /pubmed/26899008 http://dx.doi.org/10.1038/srep20807 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Sasamoto, Yuzuru Hayashi, Ryuhei Park, Sung-Joon Saito-Adachi, Mihoko Suzuki, Yutaka Kawasaki, Satoshi Quantock, Andrew J. Nakai, Kenta Tsujikawa, Motokazu Nishida, Kohji PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes |
title | PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes |
title_full | PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes |
title_fullStr | PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes |
title_full_unstemmed | PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes |
title_short | PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes |
title_sort | pax6 isoforms, along with reprogramming factors, differentially regulate the induction of cornea-specific genes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4761963/ https://www.ncbi.nlm.nih.gov/pubmed/26899008 http://dx.doi.org/10.1038/srep20807 |
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